{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,5,18]],"date-time":"2024-05-18T01:38:19Z","timestamp":1715996299723},"reference-count":28,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2007,7,25]],"date-time":"2007-07-25T00:00:00Z","timestamp":1185321600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Transfusion"],"published-print":{"date-parts":[[2007,8]]},"abstract":"<jats:p><jats:bold>BACKGROUND:<\/jats:bold> The long\u2010standing goal of a preclinical diagnostic test for transmissible spongiform encephalopathy (TSE) has recently become urgent because of the discovery that humans with variant Creutzfeldt\u2010Jakob disease can transmit disease via blood transfusions.<\/jats:p><jats:p><jats:bold>STUDY DESIGN AND METHODS:<\/jats:bold> The misfolded protein diagnostic (MPD) assay employs a pyrene\u2010labeled palindromic sequence of prion peptides that undergoes a cascade of coil to \u03b2\u2010sheet conversion in the presence of the misfolded prion protein (PrP<jats:sup>TSE<\/jats:sup>). The ability of the assay to detect PrP<jats:sup>TSE<\/jats:sup> in brain, serum, and plasma was tested. The basic protocol involved a several\u2010hour incubation of 200\u2010\u00b5L sample volumes with the peptide reagent in 96\u2010well plates, after which fluorescence was monitored by a fluorescence plate reader with an excitation wavelength of 350\u2003nm and emission scanning wavelength range of 365 to 600\u2003nm.<\/jats:p><jats:p><jats:bold>RESULTS:<\/jats:bold> Target specificity for PrP<jats:sup>TSE<\/jats:sup> was documented by correlation of assay signal with Western blot signals in brain tissue from TSE\u2010infected, normal, and knockout mice and negative assay signals by use of reagents with different peptide sequences. When applied to plasma or serum, the assay discriminated between samples from a variety of experimental and natural TSE infections compared to uninfected controls, with a sensitivity threshold of approximately 1 infectious dose per mL in pooled plasma from TSE\u2010infected mice.<\/jats:p><jats:p><jats:bold>CONCLUSIONS:<\/jats:bold> The MPD assay is a sensitive and specific test for the detection of PrP<jats:sup>TSE<\/jats:sup> that may be useful in both preclinical and clinical diagnosis of TSE diseases of animals and humans.<\/jats:p>","DOI":"10.1111\/j.1537-2995.2007.01284.x","type":"journal-article","created":{"date-parts":[[2007,7,26]],"date-time":"2007-07-26T00:02:53Z","timestamp":1185408173000},"page":"1418-1425","source":"Crossref","is-referenced-by-count":17,"title":["Detection of misfolded prion protein in blood with conformationally sensitive peptides"],"prefix":"10.1111","volume":"47","author":[{"given":"Tao","family":"Pan","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jasmeet","family":"Sethi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Craig","family":"Nelsen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alan","family":"Rudolph","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Larisa","family":"Cervenakova","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Paul","family":"Brown","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cindy S.","family":"Orser","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2007,7,25]]},"reference":[{"key":"e_1_2_7_2_2","doi-asserted-by":"publisher","DOI":"10.1038\/scientificamerican1084-50"},{"key":"e_1_2_7_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.41.1.154-161.1983"},{"key":"e_1_2_7_4_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1537-2995.1998.38998408999.x"},{"key":"e_1_2_7_5_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1537-2995.1999.39111169.x"},{"key":"e_1_2_7_6_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.0041-1132.2003.00586.x"},{"key":"e_1_2_7_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.76.9.4649-4650.2002"},{"key":"e_1_2_7_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(00)02719-7"},{"key":"e_1_2_7_9_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2005-12-5156"},{"key":"e_1_2_7_10_2","doi-asserted-by":"publisher","DOI":"10.1099\/0022-1317-83-11-2897"},{"key":"e_1_2_7_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(04)15486-X"},{"key":"e_1_2_7_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(04)16811-6"},{"key":"e_1_2_7_13_2","volume-title":"HPA 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