{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,23]],"date-time":"2026-01-23T08:18:09Z","timestamp":1769156289434,"version":"3.49.0"},"reference-count":26,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2007,6,26]],"date-time":"2007-06-26T00:00:00Z","timestamp":1182816000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Transfusion"],"published-print":{"date-parts":[[2007,10]]},"abstract":"<jats:p><jats:bold>BACKGROUND:<\/jats:bold> Blood donor parvovirus B19 DNA prevalence with sensitive nucleic acid test assays has recently been demonstrated to be higher than that found with assays designed to detect high viral titers in the plasma manufacturing sector.<\/jats:p><jats:p><jats:bold>STUDY DESIGN AND METHODS:<\/jats:bold> Stored plasma aliquots from 5020 donations collected between 2000 and 2003 at seven US blood centers were tested. Testing was performed with a real\u2010time B19 DNA polymerase chain reaction (PCR; TaqMan, Applied Biosystems) assay with a 50\u2003percent limit of detection (LOD) of 1.6\u2003IU per mL (95% confidence interval [CI], 1.2\u20102.1\u2003IU\/mL) and a 95\u2003percent LOD of 16.5\u2003IU per mL (95% CI, 10.6\u201033.9\u2003IU\/mL). Confirmation and quantitation of B19 DNA was accomplished by retesting of two additional subaliquots. Confirmed\u2010positive specimens were tested for the presence of anti\u2010B19 immunoglobulin\u2003M (IgM) and IgG with FDA\u2010licensed assays.<\/jats:p><jats:p><jats:bold>RESULTS:<\/jats:bold> B19 DNA prevalence was 0.88\u2003percent (95% CI, 0.64%\u20101.2%). Among the 23 donations with B19 DNA titers of at least 20\u2003IU per mL, the median DNA concentration was 105\u2003IU per mL with an interquartile range of 42 to 481\u2003IU per mL; the highest value was 1869\u2003IU per mL. All B19 DNA\u2013positive donations were positive for the presence of IgG and 10 (23%) were also positive for the presence of IgM; IgM seropositivity was associated with increasing DNA levels (p\u2003=\u20030.0013).<\/jats:p><jats:p><jats:bold>CONCLUSION:<\/jats:bold> Low\u2010level B19 DNA was detected in nearly 1\u2003percent of donations. The 23\u2003percent of DNA\u2010positive donations with both IgM and IgG B19 antibody most likely represent acute resolving infection, whereas those with IgG but no IgM are most consistent with a more chronic and possibly persistent phase of B19 infection.<\/jats:p>","DOI":"10.1111\/j.1537-2995.2007.01341.x","type":"journal-article","created":{"date-parts":[[2007,6,26]],"date-time":"2007-06-26T23:35:20Z","timestamp":1182900920000},"page":"1756-1764","source":"Crossref","is-referenced-by-count":73,"title":["Prevalence and quantitation of parvovirus B19 DNA levels in blood donors with a sensitive polymerase chain reaction screening assay"],"prefix":"10.1111","volume":"47","author":[{"given":"Steven H.","family":"Kleinman","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Simone A.","family":"Glynn","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tzong\u2010Hae","family":"Lee","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Leslie","family":"Tobler","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Leilani","family":"Montalvo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Deborah","family":"Todd","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Joseph E.","family":"Kiss","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Venkatakrishna","family":"Shyamala","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michael P.","family":"Busch","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"name":"National Heart, Lung, Blood Institute Retrovirus Epidemiology Donor Study (REDS\u2010II)","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2007,6,26]]},"reference":[{"key":"e_1_2_7_2_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1537-2995.2005.04387.x"},{"key":"e_1_2_7_3_2","doi-asserted-by":"crossref","unstructured":"MortimerPP.Transmission of serum parvovirus\u2010like virus by clotting\u2010factor concentrates.Lancet1983:482\u20104.","DOI":"10.1016\/S0140-6736(83)90512-3"},{"key":"e_1_2_7_4_2","doi-asserted-by":"publisher","DOI":"10.1002\/ajh.2830390315"},{"key":"e_1_2_7_5_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2141.1995.tb05427.x"},{"key":"e_1_2_7_6_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1537-2995.1995.35996029163.x"},{"key":"e_1_2_7_7_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1537-2995.1997.37997454023.x"},{"key":"e_1_2_7_8_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1423-0410.1998.7520097.x"},{"key":"e_1_2_7_9_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1537-2995.2005.00610.x"},{"key":"e_1_2_7_10_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1537-2995.2006.00952.x"},{"key":"e_1_2_7_11_2","doi-asserted-by":"publisher","DOI":"10.1086\/505226"},{"key":"e_1_2_7_12_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1423-0410.2003.00299.x"},{"key":"e_1_2_7_13_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.78.22.12169-12178.2004"},{"key":"e_1_2_7_14_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.0042-9007.2001.00124.x"},{"key":"e_1_2_7_15_2","doi-asserted-by":"publisher","DOI":"10.1086\/430442"},{"key":"e_1_2_7_16_2","doi-asserted-by":"publisher","DOI":"10.1007\/s100960000384"},{"key":"e_1_2_7_17_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2005-03-1053"},{"key":"e_1_2_7_18_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1537-2995.2005.00171.x"},{"key":"e_1_2_7_19_2","first-page":"90S","article-title":"Development of a parvovirus B19 nucleic acid quantitative confirmatory assay for use in blood donor screening [abstract]","volume":"42","author":"Shyamala V","year":"2002","journal-title":"Transfusion"},{"key":"e_1_2_7_20_2","unstructured":"Application of nucleic acid testing to blood borne pathogens and emerging technologies. 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