{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,23]],"date-time":"2026-01-23T16:02:47Z","timestamp":1769184167538,"version":"3.49.0"},"reference-count":21,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2007,8,2]],"date-time":"2007-08-02T00:00:00Z","timestamp":1186012800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Transfusion"],"published-print":{"date-parts":[[2007,11]]},"abstract":"<jats:p><jats:bold>BACKGROUND:<\/jats:bold> A multi\u2013blood center study was conducted to evaluate a human immunodeficiency virus type 1 (HIV\u20101) and hepatitis\u2003C virus (HCV) multiplex nucleic acid testing (NAT) donor screening test and to determine the residual risk for HIV\u20101 and HCV infection.<\/jats:p><jats:p><jats:bold>STUDY DESIGN AND METHODS:<\/jats:bold> A commercially available HIV\u20101 and HCV assay (Procleix, Chiron Corp.) was used for simultaneous detection of HIV\u20101 RNA and HCV RNA on 89,647 unlinked donor samples. NAT was performed with pools of 16 samples that had passed all routine screening tests. Single\u2010donor NAT was performed for samples that had been disqualified by any reactive screening test result(s). Anti\u2010HCV (Ortho third\u2010generation HCV enzyme immunoassay [EIA]), alanine aminotransferase, and HCV NAT (Roche COBAS Amplicor HCV test) confirmatory tests were used for HCV EIA\u2013nonreactive, HCV NAT\u2013reactive samples.<\/jats:p><jats:p><jats:bold>RESULTS:<\/jats:bold> Three HCV NAT yield cases and no HIV\u20101 yield cases were detected. The yield rate for HCV NAT was 3.4 per 10<jats:sup>5<\/jats:sup> (95\u2003percent confidence interval [CI], 0.7\u20109.8). The estimated incidence rate for HCV is 24.2 per 100,000 person\u2010years (95% CI, 3.4\u201088.0). If minipool NAT is added to routine donor screening, the residual risk for HCV is estimated to be reduced to 1 in 20.4\u2003\u00d7\u200310<jats:sup>4<\/jats:sup> (95% CI, 1 in 5.2\u2003\u00d7\u200310<jats:sup>4<\/jats:sup>\u20101 in 165.5\u2003\u00d7\u200310<jats:sup>4<\/jats:sup>).<\/jats:p><jats:p><jats:bold>CONCLUSION:<\/jats:bold> The residual risk for transfusion\u2010transmitted HCV infection is still relatively high in China. Incorporating NAT technology into blood donor screening would be estimated to reduce the residual risk of HCV infections eightfold over current EIA screening.<\/jats:p>","DOI":"10.1111\/j.1537-2995.2007.01424.x","type":"journal-article","created":{"date-parts":[[2007,8,7]],"date-time":"2007-08-07T01:15:53Z","timestamp":1186449353000},"page":"2011-2016","source":"Crossref","is-referenced-by-count":25,"title":["A multi\u2013Chinese blood center study testing serologic\u2010negative donor samples for hepatitis\u2003C virus and human immunodeficiency virus with nucleic acid 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