{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,5,25]],"date-time":"2024-05-25T02:11:00Z","timestamp":1716603060456},"reference-count":17,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2007,8,21]],"date-time":"2007-08-21T00:00:00Z","timestamp":1187654400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Transfusion"],"published-print":{"date-parts":[[2007,12]]},"abstract":"<jats:p><jats:bold>BACKGROUND:<\/jats:bold> Polymerization of hemoglobin (Hb) S is exacerbated by acidic and hyperosmotic citrate anticoagulant solutions and often results in occlusion of leukoreduction filters by red blood cells (RBCs) from sickle cell trait (Hb\u2003AS) donors. This study evaluates a blood collection instrument that adds citrate anticoagulant in a metered fashion, thus mitigating adverse citrate effects.<\/jats:p><jats:p><jats:bold>STUDY DESIGN AND METHODS:<\/jats:bold> Collection of whole blood by a metered anticoagulant system was compared to traditional phlebotomy in 12 Hb\u2003AS and 12 non\u2013sickle trait (Hb\u2003AA) donors. Each donated twice; on one occasion, units were filtered after 4\u2010hour storage at 20 to 24\u00b0C, and on the other, units were stored at 1 to 6\u00b0C for 24\u2003hours before filtration.<\/jats:p><jats:p><jats:bold>RESULTS:<\/jats:bold> Filtration times, RBC recoveries, and residual white blood cell (WBC) counts met defined criteria more often in Hb\u2003AS units collected by a metered anticoagulant system (9 of 12, 8 of 12, and 4 of 12, respectively) compared to traditional phlebotomy (1 of 12, 2 of 12, and 0 of 12, respectively). Overall, Hb\u2003AS units filtered better after storage at 1 to 6\u00b0C for 24\u2003hours, with units collected by a metered anticoagulant system undergoing filtration most effectively (5 of 6 had &gt;85% RBC recovery, 3 of 6 had &lt;5\u2003\u00d7\u200310<jats:sup>6<\/jats:sup> residual WBC). Units exhibited similar changes in RBC storage parameters.<\/jats:p><jats:p><jats:bold>CONCLUSION:<\/jats:bold> Use of a metered anticoagulation instrument demonstrates potential for successful leukoreduction and acceptable storage of Hb\u2003AS units; however, the system needs further modifications and improvements before it can be utilized to collect and leukoreduce Hb\u2003AS blood.<\/jats:p>","DOI":"10.1111\/j.1537-2995.2007.01452.x","type":"journal-article","created":{"date-parts":[[2007,8,21]],"date-time":"2007-08-21T16:16:19Z","timestamp":1187712979000},"page":"2233-2241","source":"Crossref","is-referenced-by-count":8,"title":["Leukoreduction filtration of whole\u2010blood units from sickle trait donors: effects of a metered citrate anticoagulant system"],"prefix":"10.1111","volume":"47","author":[{"given":"Barbara\u2003J.","family":"Bryant","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maria","family":"Bianchi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Robert\u2003A.","family":"Wesley","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"David\u2003F.","family":"Stroncek","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Susan\u2003F.","family":"Leitman","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2007,8,21]]},"reference":[{"key":"e_1_2_7_2_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1537-2995.2002.00206.x"},{"key":"e_1_2_7_3_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1537-2995.2003.00669.x"},{"key":"e_1_2_7_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.tmrv.2004.03.002"},{"key":"e_1_2_7_5_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.0041-1132.2004.04086.x"},{"key":"e_1_2_7_6_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1423-0410.2003.00344.x"},{"key":"e_1_2_7_7_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1423-0410.1997.73100543.x"},{"key":"e_1_2_7_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0955-3886(98)00056-3"},{"key":"e_1_2_7_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0955-3886(00)00017-5"},{"key":"e_1_2_7_10_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1537-2995.2001.41101192.x"},{"key":"e_1_2_7_11_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2141.2003.04464.x"},{"key":"e_1_2_7_12_2","first-page":"H2003","article-title":"Contributions of sickle hemoglobin polymer and sickle cell membranes to impaired filterability","volume":"268","author":"Hiruma H","year":"1995","journal-title":"Am J Physiol"},{"key":"e_1_2_7_13_2","first-page":"64","article-title":"Leukoreduction of red cells from donors with sickle cell trait","volume":"41","author":"Kline L","year":"2001","journal-title":"Transfusion"},{"key":"e_1_2_7_14_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI115521"},{"key":"e_1_2_7_15_2","doi-asserted-by":"publisher","DOI":"10.1093\/ajcp\/26.8.855"},{"key":"e_1_2_7_16_2","volume-title":"Automate for blood collection, ABC, equipment instruction manual","year":"2002"},{"key":"e_1_2_7_17_2","volume-title":"Leukoreduction system for blood components: CPD blood bag system with AS\u20101 additive solution, including in\u2010line Leucoflex MTL1 filter, Bactivam, Vacuvam, and Secuvam.","year":"2004"},{"key":"e_1_2_7_18_2","first-page":"767","volume-title":"StatXact.","year":"2003"}],"container-title":["Transfusion"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fj.1537-2995.2007.01452.x","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/j.1537-2995.2007.01452.x","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,14]],"date-time":"2023-10-14T11:09:30Z","timestamp":1697281770000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/j.1537-2995.2007.01452.x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2007,8,21]]},"references-count":17,"journal-issue":{"issue":"12","published-print":{"date-parts":[[2007,12]]}},"alternative-id":["10.1111\/j.1537-2995.2007.01452.x"],"URL":"https:\/\/doi.org\/10.1111\/j.1537-2995.2007.01452.x","archive":["Portico"],"relation":{},"ISSN":["0041-1132","1537-2995"],"issn-type":[{"value":"0041-1132","type":"print"},{"value":"1537-2995","type":"electronic"}],"subject":[],"published":{"date-parts":[[2007,8,21]]}}}