{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,28]],"date-time":"2026-04-28T12:47:17Z","timestamp":1777380437982,"version":"3.51.4"},"reference-count":40,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2008,5,22]],"date-time":"2008-05-22T00:00:00Z","timestamp":1211414400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Transfusion"],"published-print":{"date-parts":[[2008,8]]},"abstract":"<jats:p><jats:bold>BACKGROUND:<\/jats:bold> Transfusion\u2010transmitted cases of malaria and babesiosis have been well documented. Current efforts to screen out contaminated blood products result in component wastage due to the lack of specific detection methods while donor deferral does not always guarantee safe blood products. This study evaluated the efficacy of a photochemical treatment (PCT) method with amotosalen and long\u2010wavelength ultraviolet light (UVA) to inactivate these agents in red blood cells (RBCs) contaminating platelet (PLT) and plasma components.<\/jats:p><jats:p><jats:bold>STUDY DESIGN AND METHODS:<\/jats:bold>  <jats:italic>Plasmodium falciparum\u2013<\/jats:italic> and <jats:italic>Babesia microti<\/jats:italic>\u2013contaminated RBCs seeded into PLT and plasma components were treated with 150\u2003\u00b5mol per L amotosalen and 3\u2003J per cm<jats:sup>2<\/jats:sup> UVA. The viability of both pathogens before and after treatment was measured with infectivity assays. Treatment with 150\u2003\u00b5mol per L amotosalen and 1\u2003J per cm<jats:sup>2<\/jats:sup> UVA was used to assess the robustness of the PCT system.<\/jats:p><jats:p><jats:bold>RESULTS:<\/jats:bold> No viable <jats:italic>B. microti<\/jats:italic> was detected in PLTs or plasma after treatment with 150\u2003mol per L amotosalen and 3\u2003J per cm<jats:sup>2<\/jats:sup> UVA, demonstrating a mean inactivation of greater than 5.3 log in PLTs and greater than 5.3 log in plasma. After the same treatment, viable <jats:italic>P. falciparum<\/jats:italic> was either absent or below the limit of quantification in three of four replicate experiments both in PLTs and in plasma demonstrating a mean inactivation of at least 6.0 log in PLTs and at least 6.9 log in plasma. Reducing UVA dose to 1\u2003J per cm<jats:sup>2<\/jats:sup> did not significantly affect the level of inactivation.<\/jats:p><jats:p><jats:bold>CONCLUSION:<\/jats:bold>  <jats:italic>P. falciparum<\/jats:italic> and <jats:italic>B. microti<\/jats:italic> were highly sensitive to inactivation by PCT. Pathogen inactivation approaches could reduce the risk of transfusion\u2010transmitted parasitic infections and avoid unnecessary donor exclusions.<\/jats:p>","DOI":"10.1111\/j.1537-2995.2007.01762.x","type":"journal-article","created":{"date-parts":[[2008,5,22]],"date-time":"2008-05-22T14:29:01Z","timestamp":1211466541000},"page":"1676-1684","source":"Crossref","is-referenced-by-count":51,"title":["Photochemical inactivation with amotosalen and long\u2010wavelength ultraviolet light of <i>Plasmodium<\/i> and <i>Babesia<\/i> in platelet and plasma 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