{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,2]],"date-time":"2026-04-02T18:02:18Z","timestamp":1775152938604,"version":"3.50.1"},"reference-count":32,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2009,11,9]],"date-time":"2009-11-09T00:00:00Z","timestamp":1257724800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Transfusion"],"published-print":{"date-parts":[[2010,3]]},"abstract":"<jats:p><jats:bold>BACKGROUND:<\/jats:bold> Human immunodeficiency virus Type 1 (HIV\u20101) assays applying nucleic acid testing (NAT) rely on HIV\u20101 sequence\u2010specific primers and probes. Their hybridization can be limited or abolished by genetic polymorphisms occurring in the target sequence.<\/jats:p><jats:p><jats:bold>STUDY DESIGN AND METHODS:<\/jats:bold> Blood donations are routinely tested for HIV\u20101\/2 antibodies and for HIV\u20101 RNA in our blood transfusion unit. Recently, HIV\u20101 RNA was undetectable with an established in\u2010house real\u2010time long terminal repeat (LTR) reverse transcriptase\u2013polymerase chain reaction (RT\u2010PCR) in two cases, whereas serologic assays were positive. The reason for this discrepancy was elucidated by sequencing of the NAT target region in the respective single donations. An improved primer was designed and tested on HIV\u20101 reference panels and blood donations to ensure reliable detection of HIV\u20101 RNA.<\/jats:p><jats:p><jats:bold>RESULTS:<\/jats:bold> Direct sequencing of the target region, isolated from samples of two unrelated HIV\u2010positive blood donors, revealed one and four mismatches in the hybridization domain of the forward primer, respectively. Both viruses belong to HIV\u20101 Subtype B. LTR RT\u2010PCR with an additional forward primer was suitable for all strains of HIV\u20101 tested with high sensitivity.<\/jats:p><jats:p><jats:bold>CONCLUSIONS:<\/jats:bold> Surveillance of HIV\u20101 genetic diversity is essentially required to continually evaluate its impact on performance of diagnostic and patient monitoring assays.<\/jats:p>","DOI":"10.1111\/j.1537-2995.2009.02477.x","type":"journal-article","created":{"date-parts":[[2009,11,9]],"date-time":"2009-11-09T15:24:18Z","timestamp":1257780258000},"page":"685-692","source":"Crossref","is-referenced-by-count":18,"title":["TRANSFUSION COMPLICATIONS: Improvement of an ultrasensitive human immunodeficiency virus Type 1 real\u2010time reverse transcriptase\u2013polymerase chain reaction targeting the long terminal repeat 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