{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T16:58:06Z","timestamp":1791305886255,"version":"4.3.1"},"reference-count":27,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2010,5,4]],"date-time":"2010-05-04T00:00:00Z","timestamp":1272931200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Transfusion"],"published-print":{"date-parts":[[2010,10]]},"abstract":"<jats:p><jats:bold>BACKGROUND:<\/jats:bold> Hypoxia is a major cause of painful vaso\u2010occlusive crisis in sickle cell disease (SCD). Simple transfusion and red blood cell (RBC) exchange are commonly used as preventive therapies whose aim is to dilute hemoglobin (Hb)S\u2010containing RBCs (SS\u2010RBCs) with normal RBCs (AA\u2010RBCs) to prevent sickling. We hypothesized that the effectiveness of transfusion could be improved by the encapsulation of inositol hexaphosphate (IHP), an allosteric Hb effector, in transfused AA\u2010RBCs. Indeed, apart from their diluting effect on SS\u2010RBCs, IHP\u2010loaded RBCs (IHP\u2010RBCs) with increased oxygen release capacity could palliate in vivo oxygen deprivation and reduce sickling.<\/jats:p><jats:p><jats:bold>STUDY DESIGN AND METHODS:<\/jats:bold> The study was designed to investigate the therapeutic effect of IHP\u2010RBCs transfusion on in vitro sickling of SS\u2010RBCs collected from 20 SCD patients. Patients' RBCs were diluted with various proportions of IHP\u2010RBCs or AA\u2010RBCs (processed or stored RBCs as controls). Resulting suspensions were subjected to deoxygenation followed by partial reoxygenation at 5% oxygen. Sickling was evaluated by microscopy.<\/jats:p><jats:p><jats:bold>RESULTS:<\/jats:bold> Stored RBCs (50% dose) used to mimic simple transfusion exhibited a poor antisickling effect (5.6%) and a low response rate (65%). In contrast, IHP\u2010RBCs treatment was seven times more effective resulting in 35% of sickling reduction and a 94% response rate. Sickling was inhibited in a dose\u2010dependent manner: 9.9, 25.1, and 35.0% for IHP\u2010RBCs in percentages of 10, 30, and 50%, respectively.<\/jats:p><jats:p><jats:bold>CONCLUSION:<\/jats:bold> Our results indicate that IHP\u2010RBCs prevent in vitro sickling and suggest that it could improve conventional transfusion therapy in terms of transfused volume, frequency, and efficacy.<\/jats:p>","DOI":"10.1111\/j.1537-2995.2010.02663.x","type":"journal-article","created":{"date-parts":[[2010,5,5]],"date-time":"2010-05-05T03:49:01Z","timestamp":1273031341000},"page":"2176-2184","source":"Crossref","is-referenced-by-count":18,"title":["Inositol hexaphosphate\u2013loaded red blood cells prevent in vitro 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