{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,11]],"date-time":"2026-06-11T15:29:46Z","timestamp":1781191786629,"version":"3.54.1"},"reference-count":23,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2011,5,26]],"date-time":"2011-05-26T00:00:00Z","timestamp":1306368000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Transfusion"],"published-print":{"date-parts":[[2011,11]]},"abstract":"<jats:p><jats:bold>BACKGROUND:<\/jats:bold> Hemolysis may occur in 9% to 40% of patients after solid organ transplantation and be caused by the passenger lymphocyte syndrome (PLS).<\/jats:p><jats:p><jats:bold>STUDY DESIGN AND METHODS:<\/jats:bold> We have prospectively examined 217 kidney transplant recipients before (Day \u22121) and after (up to Days +10, +20, and +30) surgery. ABO\u2010identical transplant was performed in 180 (82.9%) patients, while 37 (17.1%) individuals received ABO\u2010compatible nonidentical grafts. Direct antiglobulin tests (DATs) were performed by tube technique (polyspecific anti\u2010human globulin [IgG\u2003+\u2003C3d]), positive DAT samples were further tested by gel agglutination (monospecific anti\u2010IgG, \u2010IgM, \u2010IgA, or \u2010C3), and eluates were prepared from DAT\u2010positive red blood cells (RBCs) by the dichloromethane elution test.<\/jats:p><jats:p><jats:bold>RESULTS:<\/jats:bold> We observed that 34 of 217 (15.7%) patients developed a positive DAT up to Day +30. The percentage of patients with positive DATs was significantly higher in those having ABO\u2010compatible nonidentical transplants compared to those that received ABO\u2010identical grafts (10\/37\u2003=\u200327.0% vs. 24\/180\u2003=\u200313.3%; p\u2003=\u20030.037). Specific RBC antibodies (anti\u2010A or anti\u2010B) were found in only 5 of 37 (13.5%) patients having ABO\u2010compatible nonidentical transplants who presented with clinical hemolysis. We found only three reactive eluates from 24 patients with positive DATs who received ABO\u2010identical transplants but had no hemolysis.<\/jats:p><jats:p><jats:bold>CONCLUSIONS:<\/jats:bold> Our data collected prospectively demonstrated that: 1) positive DATs occurred in 15.7% of all patients up to Day +30 after a kidney transplant; 2) the DAT positivity occurred up to Day +10 in 9.7% of all transplanted patients; 3) the majority of the transplant recipients with a positive DAT had a nonreactive RBC eluate; and 4) PLS was the cause of a positive DAT in 13.5% of patients submitted to ABO\u2010compatible nonidentical kidney transplants.<\/jats:p>","DOI":"10.1111\/j.1537-2995.2011.03192.x","type":"journal-article","created":{"date-parts":[[2011,5,26]],"date-time":"2011-05-26T13:25:15Z","timestamp":1306416315000},"page":"2495-2499","source":"Crossref","is-referenced-by-count":14,"title":["Hemolytic anemia after kidney transplantation: a prospective analysis"],"prefix":"10.1111","volume":"51","author":[{"given":"Ruth","family":"Achkar","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Akemi K.","family":"Chiba","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jos\u00e9 P.","family":"Zampieri\u2010Filho","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jos\u00e9 O.M.","family":"Pestana","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jos\u00e9 O.","family":"Bordin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2011,5,26]]},"reference":[{"key":"e_1_2_7_2_2","doi-asserted-by":"publisher","DOI":"10.1093\/ndt\/gfh1057"},{"key":"e_1_2_7_3_2","doi-asserted-by":"publisher","DOI":"10.1053\/j.seminhematol.2005.05.017"},{"key":"e_1_2_7_4_2","first-page":"163","article-title":"Hemolytic anemia resulting from autoantibodies produced by the donor's lymphocytes after renal transplantation","volume":"1","author":"Ahmed 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