{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T21:00:49Z","timestamp":1791320449877,"version":"4.3.1"},"reference-count":50,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2011,7,25]],"date-time":"2011-07-25T00:00:00Z","timestamp":1311552000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Transfusion"],"published-print":{"date-parts":[[2012,2]]},"abstract":"<jats:p><jats:bold>BACKGROUND:<\/jats:bold> Increased rates of red blood cell (RBC) alloimmunization in patients with sickle cell disease may be due to transfusion frequency, genetic predisposition, or immune dysregulation. To test the hypothesis that sickle cell pathophysiology influences RBC alloimmunization, we utilized two transgenic mouse models of sickle cell disease.<\/jats:p><jats:p><jats:bold>STUDY DESIGN AND METHODS:<\/jats:bold> Transgenic sickle mice, which express human \u03b1 and \u03b2<jats:sup>S<\/jats:sup> globin, were transfused with fresh or 14\u2010day\u2010stored RBCs containing the HOD (hen egg lysozyme, ovalbumin, and human Duffy<jats:sup>b<\/jats:sup>) antigen; some recipients were inflamed with poly(I\u2003:\u2003C) before transfusion. Anti\u2010HOD alloantibody responses were subsequently measured by enzyme\u2010linked immunosorbent assay and flow crossmatch; a cohort of recipients had posttransfusion serum cytokines measured by bead array.<\/jats:p><jats:p><jats:bold>RESULTS:<\/jats:bold> Both Berkeley and Townes homozygous (SS) and heterozygous (AS) mice had similar rates and magnitude of anti\u2010HOD RBC alloimmunization after fresh HOD RBC transfusion compared with control animals; under no tested condition did homozygous SS recipients make higher levels of alloantibodies than control animals. Unexpectedly, homozygous SS recipients had blunted cytokine responses and lower levels of anti\u2010HOD alloantibodies after transfusion of 14\u2010day stored RBCs, compared with control animals.<\/jats:p><jats:p><jats:bold>CONCLUSIONS:<\/jats:bold> In sum, homozygous \u03b2<jats:sup>S<\/jats:sup> expression and the ensuing disease state are not alone sufficient to enhance RBC alloimmunization to transfused HOD RBCs in two distinct humanized murine models of sickle cell disease under the conditions examined. These data suggest that other factors may contribute to the high rates of RBC alloimmunization observed in humans with sickle cell disease.<\/jats:p>","DOI":"10.1111\/j.1537-2995.2011.03255.x","type":"journal-article","created":{"date-parts":[[2011,7,26]],"date-time":"2011-07-26T18:03:50Z","timestamp":1311703430000},"page":"231-240","source":"Crossref","is-referenced-by-count":26,"title":["Alloimmunization to transfused HOD red blood cells is not increased in mice with sickle cell disease"],"prefix":"10.1111","volume":"52","author":[{"given":"Jeanne E.","family":"Hendrickson","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eldad A.","family":"Hod","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jennifer R.","family":"Perry","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Samit","family":"Ghosh","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Prasanthi","family":"Chappa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Olufolake","family":"Adisa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Leslie S.","family":"Kean","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Solomon F.","family":"Ofori\u2010Acquah","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David R.","family":"Archer","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Steven L.","family":"Spitalnik","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"James C.","family":"Zimring","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2011,7,25]]},"reference":[{"key":"e_1_2_8_2_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1537-2995.2002.00007.x"},{"key":"e_1_2_8_3_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V76.7.1431.1431"},{"key":"e_1_2_8_4_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM199006073222301"},{"key":"e_1_2_8_5_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2141.1995.tb05425.x"},{"key":"e_1_2_8_6_2","first-page":"42","article-title":"Multiple red cell transfusions and alloimmunization. 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