{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T13:14:56Z","timestamp":1790255696657,"version":"4.1.0"},"reference-count":19,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2011,10,27]],"date-time":"2011-10-27T00:00:00Z","timestamp":1319673600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Transfusion"],"published-print":{"date-parts":[[2012,5]]},"abstract":"<jats:p><jats:bold>BACKGROUND:<\/jats:bold> Recent reports have shown that the HNA\u20103a leukocyte antigen, a target for antibodies that cause severe transfusion\u2010related acute lung injury, correlates with an arginine 154 (rather than glutamine) polymorphism in choline transporter\u2013like protein 2 (CTL2) but did not show directly that R154 determines HNA\u20103a. CTL2 peptides containing R154 are recognized by only half of HNA\u20103a antibodies studied to date. Constructs that react with all HNA\u20103a antibodies are needed to fully define the HNA\u20103a epitope.<\/jats:p><jats:p><jats:bold>STUDY DESIGN AND METHODS:<\/jats:bold> HEK293 cells were transfected with cDNA encoding full\u2010length CTL2 linked to green fluorescent protein (GFP). Transfectants were selected for GFP expression and tested with antibodies specific for HNA\u20103a and \u20103b.<\/jats:p><jats:p><jats:bold>RESULTS:<\/jats:bold> Each of 20 HNA\u20103a antibodies reacted preferentially with HEK293 cells expressing the R154 CTL2 construct. An HNA\u20103b antibody reacted only with CTL2 (Q154).<\/jats:p><jats:p><jats:bold>CONCLUSIONS:<\/jats:bold> These findings provide direct evidence that R154 in the context of full\u2010length CTL2 is both necessary and sufficient to create the HNA\u20103a epitope but suggest that posttranslational modifications of the protein, for example, S\u2010S bonds or addition of glycans, are necessary for recognition of HNA\u20103a by many antibodies. This could complicate development of an assay for large\u2010scale screening of blood donors to detect anti\u2010HNA\u20103a.<\/jats:p>","DOI":"10.1111\/j.1537-2995.2011.03411.x","type":"journal-article","created":{"date-parts":[[2011,10,28]],"date-time":"2011-10-28T02:23:02Z","timestamp":1319768582000},"page":"1112-1116","source":"Crossref","is-referenced-by-count":10,"title":["Full\u2010length recombinant choline transporter\u2013like protein 2 containing arginine 154 reconstitutes the epitope recognized by HNA\u20103a antibodies"],"prefix":"10.1111","volume":"52","author":[{"given":"Adam J.","family":"Kanack","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Julie A.","family":"Peterson","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mia J.","family":"Sullivan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniel W.","family":"Bougie","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Brian R.","family":"Curtis","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Richard H.","family":"Aster","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2011,10,27]]},"reference":[{"key":"e_1_2_8_2_2","doi-asserted-by":"publisher","DOI":"10.1097\/01.CCM.0000159849.94750.51"},{"key":"e_1_2_8_3_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1399-0039.2011.01713.x"},{"key":"e_1_2_8_4_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1537-2995.2003.00374.x"},{"key":"e_1_2_8_5_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1423-0410.2008.01092.x"},{"key":"e_1_2_8_6_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2006-05-025106"},{"key":"e_1_2_8_7_2","doi-asserted-by":"publisher","DOI":"10.1038\/nm.2070"},{"key":"e_1_2_8_8_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2009-11-248336"},{"key":"e_1_2_8_9_2","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.5063-03.2004"},{"key":"e_1_2_8_10_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1537-2995.2011.03145.x"},{"key":"e_1_2_8_11_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1537-2995.2011.03115.x"},{"key":"e_1_2_8_12_2","doi-asserted-by":"publisher","DOI":"10.1007\/s10930-010-9268-y"},{"key":"e_1_2_8_13_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1537-2995.2010.02751.x"},{"key":"e_1_2_8_14_2","unstructured":"National Center for Biotechnology Information.Database of single nucleotide polymorphisms (dbSNP). Bethesda (MD):National Center for Biotechnology Information National Library of Medicine (dbSNP Build 134). Available from: URL:http:\/\/www.ncbi.nlm.nih.gov\/snp?term=Human%20CTL2"},{"key":"e_1_2_8_15_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1537-2995.2011.03155.x"},{"key":"e_1_2_8_16_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2141.1990.tb06374.x"},{"key":"e_1_2_8_17_2","doi-asserted-by":"crossref","first-page":"485","DOI":"10.1055\/s-0038-1651638","article-title":"Human platelet antigen 3 (HPA\u20103): localization of the determinant of the alloantibody Lek(a) (HPA\u20103a) to the C\u2010terminus of platelet glycoprotein IIb heavy chain and contribution of O\u2010linked carbohydrates","volume":"69","author":"Djaffar I","year":"1993","journal-title":"Thromb Haemost"},{"key":"e_1_2_8_18_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1537-2995.2007.01550.x"},{"key":"e_1_2_8_19_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1537-2995.1983.23283172846.x"},{"key":"e_1_2_8_20_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0008-6215(00)82680-4"}],"container-title":["Transfusion"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fj.1537-2995.2011.03411.x","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/j.1537-2995.2011.03411.x","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,14]],"date-time":"2023-10-14T07:11:00Z","timestamp":1697267460000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/j.1537-2995.2011.03411.x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2011,10,27]]},"references-count":19,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2012,5]]}},"alternative-id":["10.1111\/j.1537-2995.2011.03411.x"],"URL":"https:\/\/doi.org\/10.1111\/j.1537-2995.2011.03411.x","archive":["Portico"],"relation":{},"ISSN":["0041-1132","1537-2995"],"issn-type":[{"value":"0041-1132","type":"print"},{"value":"1537-2995","type":"electronic"}],"subject":[],"published":{"date-parts":[[2011,10,27]]}}}