{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,8]],"date-time":"2026-01-08T03:52:07Z","timestamp":1767844327961,"version":"3.49.0"},"reference-count":24,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2012,4,23]],"date-time":"2012-04-23T00:00:00Z","timestamp":1335139200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Transfusion"],"published-print":{"date-parts":[[2012,12]]},"abstract":"<jats:p><jats:bold>BACKGROUND:<\/jats:bold> Granulocyte mobilization and harvesting, the two major phases of granulocyte collection, have not been standardized.<\/jats:p><jats:p><jats:bold>STUDY DESIGN AND METHODS:<\/jats:bold> The data on 123 granulocyte collections were retrospectively investigated for the effect of the mobilization regimen and the harvesting technique. After a single subcutaneous dose (600\u2003\u00b5g) of granulocyte\u2013colony\u2010stimulating factor (G\u2010CSF) with (n\u2003=\u200368) or without (n\u2003=\u200340) 8\u2003mg of orally administered dexamethasone, 108 granulocyte donors underwent granulocyte collections. Moreover, 15 peripheral blood stem cell (PBSC) donors who had received 400\u2003\u00b5g\/m<jats:sup>2<\/jats:sup> or 10\u2003\u00b5g\/kg G\u2010CSF for 5 days underwent granulocyte collections on the day after the last PBSC collections (PBSC\u2010GTX donors). Granulocyte harvesting was performed by leukapheresis with (n\u2003=\u2003108) or without (n\u2003=\u200315) using high\u2010molecular\u2010weight hydroxyethyl starch (HES).<\/jats:p><jats:p><jats:bold>RESULTS:<\/jats:bold> Granulocyte donors who received mobilization with G\u2010CSF plus dexamethasone produced significantly higher granulocyte yields than those who received G\u2010CSF alone (7.2\u2003\u00d7\u200310<jats:sup>10<\/jats:sup>\u2003\u00b1\u20032.0\u2003\u00d7\u200310<jats:sup>10<\/jats:sup> vs. 5.7\u2003\u00d7\u200310<jats:sup>10<\/jats:sup>\u2003\u00b1\u20031.7\u2003\u00d7\u200310<jats:sup>10<\/jats:sup>, p\u2003=\u20030.006). PBSC\u2010GTX donors produced a remarkably high granulocyte yield (9.7\u2003\u00d7\u200310<jats:sup>10<\/jats:sup>\u2003\u00b1\u20032.3\u2003\u00d7\u200310<jats:sup>10<\/jats:sup>). The use of HES was associated with better granulocyte collection efficiency (42\u2003\u00b1\u20037.8% vs. 10\u2003\u00b1\u20039.1%, p\u2003&lt;\u20030.0001).<\/jats:p><jats:p><jats:bold>CONCLUSION:<\/jats:bold> G\u2010CSF plus dexamethasone produces higher granulocyte yields than G\u2010CSF alone. Granulocyte collection from PBSC donors appears to be a rational strategy, since it produces high granulocyte yields when the related patients are at a high risk for infection and reduces difficulties in finding granulocyte donors. HES should be used in apheresis procedures.<\/jats:p>","DOI":"10.1111\/j.1537-2995.2012.03661.x","type":"journal-article","created":{"date-parts":[[2012,4,23]],"date-time":"2012-04-23T04:46:39Z","timestamp":1335156399000},"page":"2646-2652","source":"Crossref","is-referenced-by-count":38,"title":["Impact of the mobilization regimen and the harvesting technique on the granulocyte yield in healthy donors for granulocyte transfusion therapy"],"prefix":"10.1111","volume":"52","author":[{"given":"Junko","family":"Ikemoto","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Satoshi","family":"Yoshihara","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tatsuya","family":"Fujioka","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yoshitoshi","family":"Ohtsuka","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Noriko","family":"Fujita","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Akira","family":"Kokubunji","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Noriaki","family":"Okamoto","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Junichiro","family":"Ono","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kyoko","family":"Taniguchi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Katsuji","family":"Kaida","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kazuhiro","family":"Ikegame","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Masaya","family":"Okada","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Takakuni","family":"Tanizawa","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hiroyasu","family":"Ogawa","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shunro","family":"Kai","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2012,4,23]]},"reference":[{"key":"e_1_2_8_2_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.bmt.1704630"},{"key":"e_1_2_8_3_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1537-2995.2006.00693.x"},{"key":"e_1_2_8_4_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1423-0410.2008.01159.x"},{"key":"e_1_2_8_5_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2141.2003.04583.x"},{"key":"e_1_2_8_6_2","doi-asserted-by":"publisher","DOI":"10.1002\/cncr.21929"},{"key":"e_1_2_8_7_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1537-2995.2002.00249.x"},{"key":"e_1_2_8_8_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.leu.2402007"},{"key":"e_1_2_8_9_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1537-2995.1997.37297203521.x"},{"key":"e_1_2_8_10_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1537-2995.2000.40060642.x"},{"key":"e_1_2_8_11_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1537-2995.2001.41081037.x"},{"key":"e_1_2_8_12_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1537-2995.2001.41121464.x"},{"key":"e_1_2_8_13_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1537-2995.2010.02948.x"},{"key":"e_1_2_8_14_2","doi-asserted-by":"publisher","DOI":"10.1532\/IJH97.05103"},{"key":"e_1_2_8_15_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1423-0410.2007.00971.x"},{"key":"e_1_2_8_16_2","doi-asserted-by":"publisher","DOI":"10.1007\/s12185-010-0506-z"},{"key":"e_1_2_8_17_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V86.12.4662.bloodjournal86124662"},{"key":"e_1_2_8_18_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1537-2995.1998.38698326335.x"},{"key":"e_1_2_8_19_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1537-2995.2008.01997.x"},{"key":"e_1_2_8_20_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2141.1999.01619.x"},{"key":"e_1_2_8_21_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V92.11.4366"},{"key":"e_1_2_8_22_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1537-2995.2000.40040414.x"},{"key":"e_1_2_8_23_2","doi-asserted-by":"publisher","DOI":"10.1038\/bmt.2008.237"},{"key":"e_1_2_8_24_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2009-04-218651"},{"key":"e_1_2_8_25_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.0042-9007.2003.00373.x"}],"container-title":["Transfusion"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fj.1537-2995.2012.03661.x","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/j.1537-2995.2012.03661.x","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,14]],"date-time":"2023-10-14T17:18:09Z","timestamp":1697303889000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/j.1537-2995.2012.03661.x"}},"subt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