{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,12]],"date-time":"2026-03-12T03:30:48Z","timestamp":1773286248398,"version":"3.50.1"},"reference-count":23,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2012,5,3]],"date-time":"2012-05-03T00:00:00Z","timestamp":1336003200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Transfusion"],"published-print":{"date-parts":[[2013,1]]},"abstract":"<jats:p><jats:bold>BACKGROUND:<\/jats:bold> Double\u2010cord\u2010blood transplantation (DCBT) in patients is typically accompanied by predominance of a single unit. The causative mechanism, however, is unknown. Identifying the dynamics of mixed donor chimerism in general and in specific subpopulations may help to resolve this question. We conducted studies in a mouse model to develop a new analytic method using anti\u2010human HLA Class I allele\u2013specific monoclonal antibodies (HLA\u2010MoAbs) in flow cytometry.<\/jats:p><jats:p><jats:bold>STUDY DESIGN AND METHODS:<\/jats:bold> Single\u2010cord\u2010blood transplantation or DCBT from HLA\u2010mismatched donors was performed in NOD\/SCID mice. Bone marrow (BM) and peripheral blood were collected from 3 to 20 weeks after transplantation. Donor chimerism was determined quantitatively within human platelets (hPLTs), human CD45+ (hCD45+) cells, and human myeloid and lymphocyte subsets by flow cytometry.<\/jats:p><jats:p><jats:bold>RESULTS:<\/jats:bold> Both cord donors stably engrafted in NOD\/SCID. The sensitivity to detect chimerism measured with all HLA\u2010MoAbs was 1% (&gt;10 cells\/\u00b5L). In mouse BM, the percentage of human cells measured with hCD45+ versus HLA\u2010MoAbs correlated excellently (r\u2003=\u20030.999). Donor origin could be defined with HLA\u2010MoAbs for nearly all (&gt;93.6%) human cells in mouse peripheral blood and BM in all lineages. Chimerism of hPLTs in peripheral blood correlated well with hCD45+ cells in BM enabling frequent measurement of chimerism from early after transplantation onward.<\/jats:p><jats:p><jats:bold>CONCLUSION:<\/jats:bold> This approach using HLA\u2010MoAbs enables longitudinal analysis of double\u2010mixed human chimeric populations despite low absolute concentrations of human hematopoietic cell subsets in peripheral blood and BM in mice. Lacking reactivity with mouse cells, the HLA\u2010MoAbs are suitable for use in other mouse models and in humans to identify the mechanisms involved in DCBT.<\/jats:p>","DOI":"10.1111\/j.1537-2995.2012.03678.x","type":"journal-article","created":{"date-parts":[[2012,5,4]],"date-time":"2012-05-04T03:16:06Z","timestamp":1336101366000},"page":"104-114","source":"Crossref","is-referenced-by-count":4,"title":["Validation of human monoclonal HLA Class I antibodies to evaluate the kinetics of donor chimerism in different cell subsets after double\u2010cord\u2010blood transplantation in the NOD\/SCID model"],"prefix":"10.1111","volume":"53","author":[{"given":"Yvette","family":"van Hensbergen","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Arend","family":"Mulder","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jan J.","family":"Cornelissen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anneke","family":"Brand","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2012,5,3]]},"reference":[{"key":"e_1_2_8_2_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2004-07-2717"},{"key":"e_1_2_8_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.coi.2006.07.015"},{"key":"e_1_2_8_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbmt.2010.03.010"},{"key":"e_1_2_8_5_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2003-05-1601"},{"key":"e_1_2_8_6_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.bmt.1705423"},{"key":"e_1_2_8_7_2","doi-asserted-by":"publisher","DOI":"10.1007\/s12185-009-0398-y"},{"key":"e_1_2_8_8_2","doi-asserted-by":"publisher","DOI":"10.1038\/nm.2080"},{"key":"e_1_2_8_9_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.bmt.1705933"},{"key":"e_1_2_8_10_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.bmt.1705629"},{"key":"e_1_2_8_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbmt.2009.11.013"},{"key":"e_1_2_8_12_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2009-07-228999"},{"key":"e_1_2_8_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ymthe.2004.07.029"},{"key":"e_1_2_8_14_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1537-2995.2008.02077.x"},{"key":"e_1_2_8_15_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2010-03-276212"},{"key":"e_1_2_8_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbmt.2006.08.041"},{"key":"e_1_2_8_17_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.molimm.2009.10.004"},{"key":"e_1_2_8_18_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.exphem.2005.06.019"},{"key":"e_1_2_8_19_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1537-2995.2007.01472.x"},{"key":"e_1_2_8_20_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.exphem.2006.04.009"},{"key":"e_1_2_8_21_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0008489"},{"key":"e_1_2_8_22_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.0902019"},{"key":"e_1_2_8_23_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1000583"},{"key":"e_1_2_8_24_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.174.10.6477"}],"container-title":["Transfusion"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fj.1537-2995.2012.03678.x","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/j.1537-2995.2012.03678.x","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,14]],"date-time":"2023-10-14T06:50:29Z","timestamp":1697266229000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/j.1537-2995.2012.03678.x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2012,5,3]]},"references-count":23,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2013,1]]}},"alternative-id":["10.1111\/j.1537-2995.2012.03678.x"],"URL":"https:\/\/doi.org\/10.1111\/j.1537-2995.2012.03678.x","archive":["Portico"],"relation":{},"ISSN":["0041-1132","1537-2995"],"issn-type":[{"value":"0041-1132","type":"print"},{"value":"1537-2995","type":"electronic"}],"subject":[],"published":{"date-parts":[[2012,5,3]]}}}