{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T22:18:25Z","timestamp":1790806705143,"version":"4.1.0"},"reference-count":16,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2012,11,12]],"date-time":"2012-11-12T00:00:00Z","timestamp":1352678400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Transfusion"],"published-print":{"date-parts":[[2013,8]]},"abstract":"<jats:sec><jats:title>Background<\/jats:title><jats:p>The increasing frequency of transfusion\u2010transmitted babesiosis represents a concern for the safety of the <jats:styled-content style=\"fixed-case\">US<\/jats:styled-content> blood supply. The agent responsible for the disease, the intraerythrocytic parasite <jats:styled-content style=\"fixed-case\"><jats:italic>Babesia microti<\/jats:italic><\/jats:styled-content><jats:italic>,<\/jats:italic> is naturally transmitted to humans by a tick bite and is endemic in areas of the <jats:styled-content style=\"fixed-case\">N<\/jats:styled-content>ortheast and <jats:styled-content style=\"fixed-case\">U<\/jats:styled-content>pper <jats:styled-content style=\"fixed-case\">M<\/jats:styled-content>idwest <jats:styled-content style=\"fixed-case\">U<\/jats:styled-content>nited <jats:styled-content style=\"fixed-case\">S<\/jats:styled-content>tates. In this study, we explored <jats:styled-content style=\"fixed-case\"><jats:italic>B.\u2009microti<\/jats:italic><\/jats:styled-content> seroprevalence in blood donors from different areas of <jats:styled-content style=\"fixed-case\">M<\/jats:styled-content>innesota (<jats:styled-content style=\"fixed-case\">MN<\/jats:styled-content>).<\/jats:p><\/jats:sec><jats:sec><jats:title>Study Design and Methods<\/jats:title><jats:p>We tested 2150 blood donors in <jats:styled-content style=\"fixed-case\">MN<\/jats:styled-content> for the presence of antibodies against <jats:styled-content style=\"fixed-case\"><jats:italic>B.\u2009microti<\/jats:italic><\/jats:styled-content> using an immunofluorescent assay (<jats:styled-content style=\"fixed-case\">IFA<\/jats:styled-content>). Donors identified as positive (\u226564) were also tested by real\u2010time polymerase chain reaction (<jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content>) for the presence of parasite <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content>. Seropositive donors were contacted by phone and asked questions regarding tick exposure. Donors positive by <jats:styled-content style=\"fixed-case\">IFA<\/jats:styled-content> were indefinitely deferred from donating blood.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>A total of 2150 donations were tested between <jats:styled-content style=\"fixed-case\">O<\/jats:styled-content>ctober 2010 and <jats:styled-content style=\"fixed-case\">N<\/jats:styled-content>ovember 2011. Forty\u2010two donors (2.0%) were positive by <jats:styled-content style=\"fixed-case\">IFA<\/jats:styled-content> and one was also <jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content> positive. All positive donors reported extended outdoor activities, 12 recalled finding ticks on their body, and six had flu\u2010like symptoms since their last blood draw.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>This study provides new data about <jats:styled-content style=\"fixed-case\"><jats:italic>B.\u2009microti<\/jats:italic><\/jats:styled-content> seroprevalence in <jats:styled-content style=\"fixed-case\">MN<\/jats:styled-content> blood donors. Possibly because the targeted collection areas were mostly expected to be endemic for the parasite, the observed seroprevalence levels were higher than expected, although the geographic distribution of positive donors did not completely overlap with the distribution of reported clinical cases in <jats:styled-content style=\"fixed-case\">MN<\/jats:styled-content>.<\/jats:p><\/jats:sec>","DOI":"10.1111\/j.1537-2995.2012.03948.x","type":"journal-article","created":{"date-parts":[[2012,11,12]],"date-time":"2012-11-12T21:40:35Z","timestamp":1352756435000},"page":"1698-1705","source":"Crossref","is-referenced-by-count":28,"title":["<scp><i>Babesia microti<\/i><\/scp> seroprevalence in <scp>M<\/scp>innesota blood donors"],"prefix":"10.1111","volume":"53","author":[{"given":"Laura","family":"Tonnetti","sequence":"first","affiliation":[{"name":"Transmissible Diseases Department American Red Cross Holland Laboratory  Rockville Maryland"},{"name":"Mid\u2010American Blood Service Division American Red Cross North\u2010Central Region  St Paul Minnesota"},{"name":"Northeast Division American Red Cross Blood Services  Farmington Connecticut"},{"name":"Biostatistical Design &amp; Analysis Center Division of Biostatistics University of Minnesota  Minneapolis Minnesota"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aaron M.","family":"Thorp","sequence":"additional","affiliation":[{"name":"Transmissible Diseases Department American Red Cross Holland Laboratory  Rockville Maryland"},{"name":"Mid\u2010American Blood Service Division American Red Cross North\u2010Central Region  St Paul Minnesota"},{"name":"Northeast Division American Red Cross Blood Services  Farmington Connecticut"},{"name":"Biostatistical Design &amp; Analysis Center Division of Biostatistics University of Minnesota  Minneapolis Minnesota"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Barbara","family":"Deisting","sequence":"additional","affiliation":[{"name":"Transmissible Diseases Department American Red Cross Holland Laboratory  Rockville Maryland"},{"name":"Mid\u2010American Blood Service Division American Red Cross North\u2010Central Region  St Paul Minnesota"},{"name":"Northeast Division American Red Cross Blood Services  Farmington Connecticut"},{"name":"Biostatistical Design &amp; Analysis Center Division of Biostatistics University of Minnesota  Minneapolis Minnesota"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gary","family":"Bachowski","sequence":"additional","affiliation":[{"name":"Transmissible Diseases Department American Red Cross Holland Laboratory  Rockville Maryland"},{"name":"Mid\u2010American Blood Service Division American Red Cross North\u2010Central Region  St Paul Minnesota"},{"name":"Northeast Division American Red Cross Blood Services  Farmington Connecticut"},{"name":"Biostatistical Design &amp; Analysis Center Division of Biostatistics University of Minnesota  Minneapolis Minnesota"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stephanie T.","family":"Johnson","sequence":"additional","affiliation":[{"name":"Transmissible Diseases Department American Red Cross Holland Laboratory  Rockville Maryland"},{"name":"Mid\u2010American Blood Service Division American Red Cross North\u2010Central Region  St Paul Minnesota"},{"name":"Northeast Division American Red Cross Blood Services  Farmington Connecticut"},{"name":"Biostatistical Design &amp; Analysis Center Division of Biostatistics University of Minnesota  Minneapolis Minnesota"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrew R.","family":"Wey","sequence":"additional","affiliation":[{"name":"Transmissible Diseases Department American Red Cross Holland Laboratory  Rockville Maryland"},{"name":"Mid\u2010American Blood Service Division American Red Cross North\u2010Central Region  St Paul Minnesota"},{"name":"Northeast Division American Red Cross Blood Services  Farmington Connecticut"},{"name":"Biostatistical Design &amp; Analysis Center Division of Biostatistics University of Minnesota  Minneapolis Minnesota"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"James S.","family":"Hodges","sequence":"additional","affiliation":[{"name":"Transmissible Diseases Department American Red Cross Holland Laboratory  Rockville Maryland"},{"name":"Mid\u2010American Blood Service Division American Red Cross North\u2010Central Region  St Paul Minnesota"},{"name":"Northeast Division American Red Cross Blood Services  Farmington Connecticut"},{"name":"Biostatistical Design &amp; Analysis Center Division of Biostatistics University of Minnesota  Minneapolis Minnesota"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David A.","family":"Leiby","sequence":"additional","affiliation":[{"name":"Transmissible Diseases Department American Red Cross Holland Laboratory  Rockville Maryland"},{"name":"Mid\u2010American Blood Service Division American Red Cross North\u2010Central Region  St Paul Minnesota"},{"name":"Northeast Division American Red Cross Blood Services  Farmington Connecticut"},{"name":"Biostatistical Design &amp; Analysis Center Division of Biostatistics University of Minnesota  Minneapolis Minnesota"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David","family":"Mair","sequence":"additional","affiliation":[{"name":"Transmissible Diseases Department American Red Cross Holland Laboratory  Rockville Maryland"},{"name":"Mid\u2010American Blood Service Division American Red Cross North\u2010Central Region  St Paul Minnesota"},{"name":"Northeast Division American Red Cross Blood Services  Farmington Connecticut"},{"name":"Biostatistical Design &amp; Analysis Center Division of Biostatistics University of Minnesota  Minneapolis Minnesota"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2012,11,12]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.2003.68.431"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.3201\/eid1705.101334"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.00022-10"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.7326\/0003-4819-155-8-201110180-00362"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1537-2995.2009.02430.x"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1537-2995.2005.00609.x"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1537-2995.2009.02317.x"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1537-2995.2002.00251.x"},{"key":"e_1_2_9_10_1","unstructured":"Minnesota Department of Health.Minnesota Department of Health diseases control newsletter. January\/July2010. Volume 38 Number 1."},{"key":"e_1_2_9_11_1","unstructured":"Wisconsin Department of Health.Babesia reported in Wisconsin 2000\u20102011.2012."},{"key":"e_1_2_9_12_1","unstructured":"Minnesota Department of Health.Minnesota Department of Health diseases control newsletter January\/August2011. Volume 39 Number 1."},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1537-2995.2012.03612.x"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1537-2995.2002.00189.x"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.amjmed.2011.03.009"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM198011063031906"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00116466"}],"container-title":["Transfusion"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fj.1537-2995.2012.03948.x","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/j.1537-2995.2012.03948.x","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,1]],"date-time":"2023-10-01T10:16:44Z","timestamp":1696155404000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/j.1537-2995.2012.03948.x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2012,11,12]]},"references-count":16,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2013,8]]}},"alternative-id":["10.1111\/j.1537-2995.2012.03948.x"],"URL":"https:\/\/doi.org\/10.1111\/j.1537-2995.2012.03948.x","archive":["Portico"],"relation":{},"ISSN":["0041-1132","1537-2995"],"issn-type":[{"value":"0041-1132","type":"print"},{"value":"1537-2995","type":"electronic"}],"subject":[],"published":{"date-parts":[[2012,11,12]]}}}