{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,17]],"date-time":"2025-10-17T13:26:57Z","timestamp":1760707617987},"reference-count":43,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2006,1,18]],"date-time":"2006-01-18T00:00:00Z","timestamp":1137542400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Risk Analysis"],"published-print":{"date-parts":[[2006,2]]},"abstract":"<jats:p>Recently, the lag phase research in predictive microbiology is focusing more on the individual cell variability, especially for pathogenic microorganisms that typically occur in very low contamination levels, like <jats:italic>Listeria monocytogenes<\/jats:italic>. In this study, the effect of this individual cell lag phase variability was introduced in an exposure assessment study for <jats:italic>L. monocytogenes<\/jats:italic> in a liver p\u00e2t\u00e9. A basic framework was designed to estimate the contamination level of p\u00e2t\u00e9 at the time of consumption, taking into account the frequency of contamination and the initial contamination levels of p\u00e2t\u00e9 at retail. Growth was calculated on p\u00e2t\u00e9 units of 150 g, comparing an individual\u2010based approach with a classical population\u2010based approach. The two different protocols were compared using simulations. If only the individual cell lag variability was taken into account, important differences were observed in cell density at the time of consumption between the individual\u2010based approach and the classical approach, especially at low inoculum levels, resulting in high variability when using the individual\u2010based approach. Although, when all variable factors were taken into account, no significant differences were observed between the different approaches, allowing the conclusion that the individual cell lag phase variability was overruled by the global variability of the exposure assessment framework. Even in more extreme conditions like a low inoculum level or a low water activity, no differences were created in cell density at the time of consumption between the individual\u2010based approach and the classical approach. This means that the individual cell lag phase variability of <jats:italic>L. monocytogenes<\/jats:italic> has important consequences when studying specific growth cases, especially when the applied inoculum levels are low, but when performing more general exposure assessment studies, the variability between the individual cell lag phases is too limited to have a major impact on the total exposure assessment.<\/jats:p>","DOI":"10.1111\/j.1539-6924.2006.00716.x","type":"journal-article","created":{"date-parts":[[2006,1,18]],"date-time":"2006-01-18T15:54:04Z","timestamp":1137599644000},"page":"105-114","source":"Crossref","is-referenced-by-count":21,"title":["Risk Assessment of <i>Listeria monocytogenes<\/i>: Impact of Individual Cell Variability on the Exposure Assessment Step"],"prefix":"10.1111","volume":"26","author":[{"given":"K.","family":"Francois","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"F.","family":"Devlieghere","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"Uyttendaele","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J.","family":"Debevere","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2006,1,18]]},"reference":[{"key":"e_1_2_5_2_1","volume-title":"Quantitative Assessment of the Relative Risk to Public Health from Foodborne Listeria Monocytogenes Among Selected Categories of Ready\u2010to\u2010Eat Foods","author":"Anonymous","year":"2003"},{"key":"e_1_2_5_3_1","unstructured":"Anonymous. (2005).European Commission. Draft Commission Regulation on Microbiological Criteria for Foodstuffs. SANCO\/1498\/2001 Rev. 15. Brussels March 15 2005."},{"key":"e_1_2_5_4_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.66.4.1706-1710.2000"},{"key":"e_1_2_5_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0168-1605(00)00260-9"},{"key":"e_1_2_5_6_1","doi-asserted-by":"publisher","DOI":"10.1006\/jtbi.1998.0673"},{"key":"e_1_2_5_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0168-1605(01)00650-X"},{"key":"e_1_2_5_8_1","doi-asserted-by":"publisher","DOI":"10.4315\/0362-028X-67.9.1967"},{"key":"e_1_2_5_9_1","first-page":"337","volume-title":"Foodborne Pathogens: Hazards, Risk Analysis and Control","author":"Bell C.","year":"2002"},{"issue":"8","key":"e_1_2_5_10_1","doi-asserted-by":"crossref","first-page":"918","DOI":"10.4315\/0362-028X-60.8.918","article-title":"Use of epidemiologic and food survey data to estimate a purposefully conservative dose\u2010response relationship for Listeria monocytogenes levels and incidence of listeriosis","volume":"60","author":"Buchanan R. L.","year":"1997","journal-title":"Journal of Food Protection"},{"key":"e_1_2_5_11_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.70.2.675-678.2004"},{"key":"e_1_2_5_12_1","doi-asserted-by":"crossref","first-page":"476","DOI":"10.1128\/mr.55.3.476-511.1991","article-title":"Listeria monocytogenes: A food\u2010borne pathogen","volume":"55","author":"Farber J. M.","year":"1991","journal-title":"Microbioliogical Reviews"},{"key":"e_1_2_5_13_1","first-page":"21","volume-title":"Listeria, Listeriosis and Food Safety","author":"Fenlon R. D.","year":"1999"},{"key":"e_1_2_5_14_1","doi-asserted-by":"publisher","DOI":"10.17660\/ActaHortic.2005.674.3"},{"key":"e_1_2_5_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijfoodmicro.2004.10.032"},{"key":"e_1_2_5_16_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1472-765X.2003.01340.x"},{"key":"e_1_2_5_17_1","article-title":"Effect of environmental parameters (temperature, pH & a\n                  \n                     w\n                  ) on the individual cell lag phase and generation time of Listeria monocytogenes","author":"Francois K.","year":"2005","journal-title":"International Journal of Food Microbiology"},{"key":"e_1_2_5_18_1","article-title":"Single cell variability of L. monocytogenes grown on p\u00e2t\u00e9 and ham at 7\u00b0C: Comparing experimental data to predictive simulations","author":"Francois K.","year":"2005","journal-title":"Journal of Applied Microbiology"},{"key":"e_1_2_5_19_1","doi-asserted-by":"publisher","DOI":"10.4315\/0362-028X-52.10.696"},{"key":"e_1_2_5_20_1","doi-asserted-by":"publisher","DOI":"10.4315\/0362-028X-66.4.559"},{"key":"e_1_2_5_21_1","doi-asserted-by":"publisher","DOI":"10.1080\/02652030110050104"},{"key":"e_1_2_5_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jtbi.2004.08.013"},{"key":"e_1_2_5_23_1","unstructured":"Leporq B. Membr\u00e9 J.\u2010M. Zwietering M. Dervin C. Buche P. &Guyonnet J. P.(2003).The \u201cSym'Previus\u201d software a tool to support decisions to the foodstuff safety. InJ. F.Van Impe A. H.Geeraerd I.Legu\u00e9rinel &P.Mafart(Eds.) 4th International Conference on Predictive Modelling in Foods Conference Proceedings(pp.55\u201357). Belgium : Katholieke Universiteit Leuven ."},{"key":"e_1_2_5_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0168-1605(97)01266-X"},{"key":"e_1_2_5_25_1","unstructured":"M\u00e9tris A. George S. Peck M. &Baranyi J.(2002).Effect of sodium chloride and pH on the distribution of the lag times of individual cells ofListeria innocua. InConference proceedings: 7\u00e8me journ\u00e9es Europ\u00e9ennes Agro\u2010industrie et methodes statistiques Lille 16 \u2013 18 janvier 2002(pp.61\u201366)."},{"key":"e_1_2_5_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mimet.2003.08.006"},{"key":"e_1_2_5_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0168-1605(99)00157-9"},{"issue":"2","key":"e_1_2_5_28_1","doi-asserted-by":"crossref","first-page":"244","DOI":"10.4315\/0362-028X-61.2.244","article-title":"Studies on the risk assessment of Listeria monocytogenes","volume":"61","author":"Notermans S.","year":"1998","journal-title":"Journal of Food Protection"},{"key":"e_1_2_5_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijfoodmicro.2003.12.002"},{"key":"e_1_2_5_30_1","doi-asserted-by":"crossref","first-page":"990","DOI":"10.1128\/jcm.29.5.990-996.1991","article-title":"Cytopathogenic effects in enterocyte caco\u20102 cells differentiate virulent from avirulent Listeria strains","volume":"29","author":"Pine L.","year":"1991","journal-title":"Journal of Clinical Microbiology"},{"key":"e_1_2_5_31_1","doi-asserted-by":"crossref","first-page":"2940","DOI":"10.1128\/iai.58.9.2940-2945.1990","article-title":"Listeria monocytogenes intragastric and intraperitoneal approximate 50% lethal doses for mice are comparable, but death occurs earlier by intragastric feeding","volume":"58","author":"Pine L.","year":"1990","journal-title":"Infection and Immunity"},{"key":"e_1_2_5_32_1","first-page":"141","volume-title":"ICMFS, Micro\u2010organisms in Food 5, Microbiological Specifications of Food Pathogens","author":"Roberts T. A.","year":"1996"},{"key":"e_1_2_5_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0168-1605(01)00541-4"},{"key":"e_1_2_5_34_1","first-page":"1","volume-title":"Listeria, Listeriosis and Food Safety","author":"Rocourt J.","year":"1999"},{"key":"e_1_2_5_35_1","doi-asserted-by":"publisher","DOI":"10.1111\/1539-6924.00299"},{"key":"e_1_2_5_36_1","first-page":"299","volume-title":"Listeria, Listeriosis and Food Safety","author":"Ryser E. T.","year":"1999"},{"key":"e_1_2_5_37_1","first-page":"75","volume-title":"Listeria, Listeriosis and Food Safety","author":"Slutsker L.","year":"1999"},{"key":"e_1_2_5_38_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0168-1605(01)00651-1"},{"key":"e_1_2_5_39_1","article-title":"Modelling the lag time distributions for Listeria monocytogenes","author":"Standaert A. R.","year":"2005","journal-title":"Journal of the Royal Statistical Society Series C Applied Statistics to Risk Analysis"},{"key":"e_1_2_5_40_1","doi-asserted-by":"crossref","first-page":"2085","DOI":"10.1128\/jcm.25.11.2085-2089.1987","article-title":"Pathogenicity test for Listeria monocytogenes using immunocompromised mice","volume":"25","author":"Stelma G. N.","year":"1987","journal-title":"Journal of Clinical Microbiology"},{"key":"e_1_2_5_41_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0168-1605(99)00155-5"},{"key":"e_1_2_5_42_1","doi-asserted-by":"publisher","DOI":"10.1006\/fmic.1997.0100"},{"key":"e_1_2_5_43_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.0272-4332.2005.00568.x"},{"key":"e_1_2_5_44_1","first-page":"272","volume-title":"Risk Analysis. A Quantitative Guide","author":"Vose D.","year":"2000"}],"container-title":["Risk Analysis"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fj.1539-6924.2006.00716.x","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/j.1539-6924.2006.00716.x","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,12]],"date-time":"2023-10-12T07:14:30Z","timestamp":1697094870000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/j.1539-6924.2006.00716.x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2006,1,18]]},"references-count":43,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2006,2]]}},"alternative-id":["10.1111\/j.1539-6924.2006.00716.x"],"URL":"https:\/\/doi.org\/10.1111\/j.1539-6924.2006.00716.x","archive":["Portico"],"relation":{},"ISSN":["0272-4332","1539-6924"],"issn-type":[{"value":"0272-4332","type":"print"},{"value":"1539-6924","type":"electronic"}],"subject":[],"published":{"date-parts":[[2006,1,18]]}}}