{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,2]],"date-time":"2025-11-02T19:50:50Z","timestamp":1762113050993},"reference-count":39,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2007,4,20]],"date-time":"2007-04-20T00:00:00Z","timestamp":1177027200000},"content-version":"vor","delay-in-days":2514,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cardiovasc electrophysiol"],"published-print":{"date-parts":[[2000,6]]},"abstract":"<jats:p>Reentry in Cell Monolayers Imaged by CFI. <jats:italic>Introduction:<\/jats:italic> We present a novel contact fluorescence imaging (CFI) approach to monitor transmembrane potentials in monolayers of cultured neonatal rat ventricular cells. We apply CFI to demonstrate, for the first time, long\u2010term recordings as well as electrical induction and termination of reentrant activity in this in vitro model.<\/jats:p><jats:p> <jats:italic>Methods and Results:<\/jats:italic> CFI was performed in confluent cell monolayers stained with di\u20108\u2010ANEPPS. An anatomic obstacle (6 \u00d7 0.5 mm) was created in the center of the monolayers. Reentry was induced with a premature stimulus after pacing at 2 Hz (both via field stimulation). Seven sustained (&gt;3 min) reentrant episodes, anchored to the anatomic obstacle, were observed in three monolayers. Field stimulation (30 V\/cm) was applied to successfully terminate 6 of the 7 reentries. Analysis of reentrant activity showed similarities with anatomic reentry in tissue preparations, such as reduced conduction velocity around the core, variable conduction velocity along the reentrant pathway due to wavefront curvature effects, and field\u2010induced activation at the obstacle borders leading to reentry termination (cardioversion).<\/jats:p><jats:p> <jats:italic>Conclusion:<\/jats:italic> This study demonstrates the feasibility of CFI for macroscopic optical mapping of transmembrane potentials in a single layer of cultured cells. Our results suggest that the monolayer cell culture model is an attractive complement to tissue models of reentry and cardioversion.<\/jats:p>","DOI":"10.1111\/j.1540-8167.2000.tb00029.x","type":"journal-article","created":{"date-parts":[[2007,4,20]],"date-time":"2007-04-20T13:13:08Z","timestamp":1177074788000},"page":"665-676","source":"Crossref","is-referenced-by-count":69,"title":["Contact Fluorescence Imaging of Reentry in Monolayers of Cultured Neonatal Rat Ventricular Myocytes"],"prefix":"10.1111","volume":"11","author":[{"given":"EMILIA","family":"ENTCHEVA","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"STEVEN N.","family":"LU","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"RACHEL H.","family":"TROPPMAN","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"VINOD","family":"SHARMA","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"LESLIE","family":"TUNG","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2007,4,20]]},"reference":[{"key":"e_1_2_1_2_2","first-page":"481","article-title":"The cause of pulsation","volume":"73","author":"Mayer A","year":"1908","journal-title":"Pop Sci Monthly"},{"key":"e_1_2_1_3_2","first-page":"43","article-title":"On circulating excitations in heart muscles and their possible relation to tachycardia and fibrillation","volume":"4","author":"Mines G","year":"1914","journal-title":"Trans R Soc Can"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1161\/01.RES.58.4.495"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.1161\/01.RES.81.5.753"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1161\/01.CIR.93.3.603"},{"key":"e_1_2_1_7_2","doi-asserted-by":"publisher","DOI":"10.1038\/355349a0"},{"key":"e_1_2_1_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-3495(96)79691-1"},{"key":"e_1_2_1_9_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.275.5301.841"},{"key":"e_1_2_1_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-3495(98)77596-4"},{"key":"e_1_2_1_11_2","doi-asserted-by":"publisher","DOI":"10.1161\/01.RES.75.3.591"},{"key":"e_1_2_1_12_2","doi-asserted-by":"publisher","DOI":"10.1161\/01.RES.79.4.676"},{"key":"e_1_2_1_13_2","doi-asserted-by":"publisher","DOI":"10.1161\/01.RES.82.3.375"},{"key":"e_1_2_1_14_2","doi-asserted-by":"publisher","DOI":"10.1161\/01.RES.68.1.114"},{"key":"e_1_2_1_15_2","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.1041610112"},{"key":"e_1_2_1_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-3495(98)77490-9"},{"key":"e_1_2_1_17_2","first-page":"66","article-title":"Unidirectional conduction block and extrasystoles in synthetic strands of cultured heart cells","volume":"23","author":"Shigeto N","year":"1982","journal-title":"Jpn Heart J"},{"key":"e_1_2_1_18_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.95.17.10283"},{"key":"e_1_2_1_19_2","doi-asserted-by":"publisher","DOI":"10.1161\/01.RES.41.1.9"},{"key":"e_1_2_1_20_2","doi-asserted-by":"crossref","first-page":"842","DOI":"10.1161\/01.RES.69.3.842","article-title":"Optical recordings in the rabbit heart show that defibrillation strength shocks prolong the duration of depolarization and the refractory period","volume":"69","author":"Dillon SM","year":"1991","journal-title":"Circ Res"},{"key":"e_1_2_1_21_2","doi-asserted-by":"publisher","DOI":"10.1038\/32170"},{"key":"e_1_2_1_22_2","unstructured":"LuSN EntchevaE SharmaV TungL:Optical mapping of anatomical reentry in monolayers of cultured neonatal rat cardiac myocytes.IEEE Engineering in Medicine and Biology International Annual Conference and BMES Annual Conference. 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