{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,6]],"date-time":"2026-01-06T02:11:38Z","timestamp":1767665498140},"reference-count":54,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2012,8,5]],"date-time":"2012-08-05T00:00:00Z","timestamp":1344124800000},"content-version":"vor","delay-in-days":4,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microcirculation"],"published-print":{"date-parts":[[2012,8]]},"abstract":"<jats:sec><jats:label \/><jats:p><jats:italic>Please cite this paper as<\/jats:italic>: Gaynes B, Teng P\u2010Y, Wanek J, Shahidi M. Feasibility of conjunctival hemodynamic measurements in rabbits: reproducibility, validity, and response to acute hypotension. <jats:italic>Microcirculation<\/jats:italic>\u00a0<jats:bold>19<\/jats:bold>: 521\u2013529, 2012.<\/jats:p><\/jats:sec><jats:sec><jats:title>Abstract<\/jats:title><jats:p><jats:bold>Objective:\u2002<\/jats:bold> To evaluate the feasibility of conjunctival hemodynamic measurements based on assessment of reproducibility, validity, and response to acute hypotension.<\/jats:p><jats:p><jats:bold>Methods:\u2002<\/jats:bold> Image sequences of the conjunctival microvasculature of rabbits were captured using a slit lamp biomicroscope under a steady\u2010state condition, after topical administration of phenylephrine, and after intravenous administration of esmolol. Venous hemodynamic parameters (diameter, blood velocity, blood flow, and wall shear stress) were derived.<\/jats:p><jats:p><jats:bold>Results:\u2002<\/jats:bold> Conjunctival venous diameters ranged from 9 to 34\u2003\u03bcm and blood velocities ranged from 0.08 to 0.95\u2003mm\/s. Coefficients of variation of venous diameter and blood velocity measurements were, on average, 6% and 14%, respectively. Automated and manual measurements of venous diameter and velocity were highly correlated (<jats:italic>R<\/jats:italic>\u2003=\u20030.97; <jats:italic>p<\/jats:italic>\u2003&lt;\u20030.001; <jats:italic>n<\/jats:italic>\u2003=\u200316). With phenylephrine administration, diameter and velocity were reduced by 21% and 69%, respectively. Following esmolol administration, blood pressure was reduced with a concomitant decrease in velocity, followed by recovery to baseline. Venous blood velocity, flow, and WSS were correlated with blood pressure (<jats:italic>R<\/jats:italic>\u2003\u2265\u20030.52; <jats:italic>p<\/jats:italic>\u2003\u2264\u20030.01).<\/jats:p><jats:p><jats:bold>Conclusions:\u2002<\/jats:bold> The feasibility of quantifying alterations in microvascular hemodynamics in the bulbar conjunctiva was established. The method is of potential value in evaluating microcirculatory hemodynamics related to cardiovascular function.<\/jats:p><\/jats:sec>","DOI":"10.1111\/j.1549-8719.2012.00182.x","type":"journal-article","created":{"date-parts":[[2012,4,5]],"date-time":"2012-04-05T18:52:57Z","timestamp":1333651977000},"page":"521-529","source":"Crossref","is-referenced-by-count":10,"title":["Feasibility of Conjunctival Hemodynamic Measurements in Rabbits: Reproducibility, Validity, and Response to Acute 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