{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T04:25:22Z","timestamp":1790915122931,"version":"4.1.0"},"reference-count":12,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2006,5,23]],"date-time":"2006-05-23T00:00:00Z","timestamp":1148342400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Eukaryotic Microbiology"],"published-print":{"date-parts":[[2006,7]]},"abstract":"<jats:p><jats:bold>ABSTRACT. <\/jats:bold> Several studies from developed countries have documented the association between trimethoprim\u2010sulfamethoxazole prophylaxis failure and mutations in the <jats:italic>Pneumocystis jirovecii<\/jats:italic> gene coding for dihydropteroate synthase (DHPS). DNA was extracted from Giemsa\u2010stained smears of 70 patients with <jats:italic>P. jirovecii<\/jats:italic> pneumonia seen in Porto Alegre, Brazil, from 1997 to 2004. Successful PCR amplification of the DHPS locus was obtained in 57 of 70 cases (81.4%), including five cases (8.7%) that had used sulfa prophylaxis. No DHPS gene mutations were seen. These results suggest that DHPS mutations are currently as rare in Brazil as in other developing countries.<\/jats:p>","DOI":"10.1111\/j.1550-7408.2006.00104.x","type":"journal-article","created":{"date-parts":[[2006,5,23]],"date-time":"2006-05-23T14:08:31Z","timestamp":1148393311000},"page":"305-307","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":22,"title":["Absence of Dihydropteroate Synthase Mutations in <i>Pneumocystis jirovecii<\/i> from Brazilian AIDS Patients"],"prefix":"10.1111","volume":"53","author":[{"given":"GUSTAVO","family":"WISSMANN","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M\u00cdRIAM J.","family":"ALVAREZ\u2010MARTINEZ","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"STEVEN R.","family":"MESHNICK","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"ADA R. 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