{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,13]],"date-time":"2026-06-13T16:35:08Z","timestamp":1781368508143,"version":"3.54.1"},"reference-count":14,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2011,11,11]],"date-time":"2011-11-11T00:00:00Z","timestamp":1320969600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Eukaryotic Microbiology"],"published-print":{"date-parts":[[2012,1]]},"abstract":"Abstract<\/jats:title>The microsporidium P<\/jats:italic><\/jats:styled-content>seudoloma neurophilia<\/jats:italic> was initially reported to infect the central nervous system of zebrafish causing lordosis and eventually death. Subsequently, muscle tissue infections were also identified. To understand the infection process, development, and ultrastructural pathology of this microsporidium, larval and adult zebrafish were fed P<\/jats:italic><\/jats:styled-content>. neurophilia<\/jats:italic> spores. Spores were detected in the larval fish digestive tract 3\u2010h postexposure (PE<\/jats:styled-content>). By 4.5\u2010d PE<\/jats:styled-content>, developing parasite stages were identified in muscle tissue. Wet preparations of larvae collected at 8\u2010d PE<\/jats:styled-content> showed aggregates of spores in the spinal cord adjacent to the notochord. All parasite stages, including spores, were present in the musculature of larval fish 8\u2010d PE<\/jats:styled-content>. Adult zebrafish sacrificed 45\u2010d PE<\/jats:styled-content> had fully developed infections in nerves. Ultrastructural study of the developmental cycle of P<\/jats:italic><\/jats:styled-content>. neurophilia<\/jats:italic> revealed that proliferative stages undergo karyokinesis, producing tetranucleate stages that then divide into uninucleate cells. The plasmalemma of proliferative cells has a previously unreported glycocalyx\u2010like coat that interfaces with the host cell cytoplasm. Sporogonic stages form sporophorous vacuoles (SPOV<\/jats:styled-content>) derived from the plasmalemmal dense surface coat, which \u201cblisters\u201d off sporonts. Uninucleate sporoblasts and spores develop in the SPOV<\/jats:styled-content>. The developmental cycle is identical in both nerve and muscle. The SPOV<\/jats:styled-content> surface is relatively thick and is the outermost parasite surface entity; thus, xenomas are not formed. Based on the new information provided by this study, the taxonomic description of the genus P<\/jats:italic><\/jats:styled-content>seudoloma<\/jats:italic> and its type species,<\/jats:italic> P<\/jats:italic><\/jats:styled-content>. neurophilia,<\/jats:italic> is modified and its life cycle described.<\/jats:p>","DOI":"10.1111\/j.1550-7408.2011.00591.x","type":"journal-article","created":{"date-parts":[[2011,11,12]],"date-time":"2011-11-12T00:44:05Z","timestamp":1321058645000},"page":"40-48","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":20,"title":["Development, Ultrastructural Pathology, and Taxonomic Revision of the Microsporidial Genus, P<\/i><\/scp>seudoloma<\/i> and Its Type Species P<\/i><\/scp>seudoloma neurophilia,<\/i> in Skeletal Muscle and Nervous Tissue of Experimentally Infected Zebrafish D<\/i><\/scp>anio rerio<\/i>"],"prefix":"10.1111","volume":"59","author":[{"given":"Ann","family":"Cali","sequence":"first","affiliation":[{"name":"Department of Biological Sciences Rutgers University Newark New Jersey 07102"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael","family":"Kent","sequence":"additional","affiliation":[{"name":"Departments of Microbiology & Biomedical Sciences Oregon State University Corvallis Oregon 97331 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Justin","family":"Sanders","sequence":"additional","affiliation":[{"name":"Departments of Microbiology & Biomedical Sciences Oregon State University Corvallis Oregon 97331 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cyrilla","family":"Pau","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences Rutgers University Newark New Jersey 07102"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter M.","family":"Takvorian","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences Rutgers University Newark New Jersey 07102"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2011,11,11]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1128\/9781555818227.ch3"},{"key":"e_1_2_7_3_1","volume-title":"Handbook of Protoctista","author":"Cali A.","year":"2011"},{"key":"e_1_2_7_4_1","first-page":"289","volume-title":"The Microsporidia of Vertebrates","author":"Canning E. 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