{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,9]],"date-time":"2026-07-09T15:40:08Z","timestamp":1783611608938,"version":"3.55.0"},"reference-count":38,"publisher":"Oxford University Press (OUP)","issue":"2","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2007,3]]},"DOI":"10.1111\/j.1574-695x.2006.00191.x","type":"journal-article","created":{"date-parts":[[2007,1,24]],"date-time":"2007-01-24T12:01:43Z","timestamp":1169640103000},"page":"184-191","source":"Crossref","is-referenced-by-count":46,"title":["Molecular methods used in clinical laboratory: prospects and pitfalls"],"prefix":"10.1093","volume":"49","author":[{"given":"Muhammad G.","family":"Morshed","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Min-Kuang","family":"Lee","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Danielle","family":"Jorgensen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Judith L.","family":"Isaac-Renton","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","reference":[{"key":"10.1111\/j.1574-695X.2006.00191.x-BIB1","doi-asserted-by":"crossref","unstructured":"Eisler DL , McNabb A , Jorgensen DR & Isaac-Renton JL (2004) Use of an internal positive control in a multiplex reverse transcription-PCR to detect West Nile virus RNA in mosquito pools. J Clin Microbiol 42: 841\u00e2\u0080\u0093843.","DOI":"10.1128\/JCM.42.2.841-843.2004"},{"key":"10.1111\/j.1574-695X.2006.00191.x-BIB2","unstructured":"Elbeik T , Surtihadi J , Destree M et al. (2004) Multicenter evaluation of the performance characteristic of the Bayer VERSANT HCV RNA 3.0 assay (bDNA). J Clin Microbiol 42: 563\u00e2\u0080\u0093569."},{"key":"10.1111\/j.1574-695X.2006.00191.x-BIB3","doi-asserted-by":"crossref","unstructured":"Esteban JI , Gomez J , Martell M , Cabot B , Quer J , Gonzalez A , Otero T , Moya A , Esteban R & Guardia J (1996) Transmission of Hepatitis C virus by a cardiac surgeon. N Engl J Med 334: 555\u00e2\u0080\u0093560.","DOI":"10.1056\/NEJM199602293340902"},{"key":"10.1111\/j.1574-695X.2006.00191.x-BIB4","doi-asserted-by":"crossref","unstructured":"Ginocchio CC , Wany X-P , Kaplan MH , Mulligan G , Witt D , Romano JW , Cronin M & Carroll R (1997) Effects of specimen collection, processing and storage conditions on stability of human immunodeficiency virus type 1 RNA levels in plasma. J Clin Microbiol 35: 2886\u00e2\u0080\u00932893.","DOI":"10.1128\/JCM.35.11.2886-2893.1997"},{"key":"10.1111\/j.1574-695X.2006.00191.x-BIB5","doi-asserted-by":"crossref","unstructured":"Grant PR , Kitchen A , Barbara JA , Hewitt P , Sims CM , Garson JA & Tedder RS (2000) Effects of handling and storage of blood on the stability of Hepatitis C virus RNA: implications for NAT testing in transfusion practice. Vox Sang 78: 137\u00e2\u0080\u0093142.","DOI":"10.1046\/j.1423-0410.2000.7830137.x"},{"key":"10.1111\/j.1574-695X.2006.00191.x-BIB6","doi-asserted-by":"crossref","unstructured":"Halfon P , Khiri H , Gerolami V , Bourliere M , Feryn JM , Reynier P , Gauthier A & Cartouzou G (1996) Impact of various handling and storage conditions on quantitative detection of Hepatitis C virus RNA. J Hepatol 25: 307\u00e2\u0080\u0093311.","DOI":"10.1016\/S0168-8278(96)80116-4"},{"key":"10.1111\/j.1574-695X.2006.00191.x-BIB7","doi-asserted-by":"crossref","unstructured":"Harpaz R , VonSeidlein L , Averhoff FM et al. (1996) Transmission of Hepatitis B virus to multiple patients from a surgeon without evidence of inadequate infection control. N Engl J Med 334: 549\u00e2\u0080\u0093554.","DOI":"10.1056\/NEJM199602293340901"},{"key":"10.1111\/j.1574-695X.2006.00191.x-BIB8","volume-title":"Infectious Disease Epidemiology: Theory and Practice","author":"Harrington","year":"2004"},{"key":"10.1111\/j.1574-695X.2006.00191.x-BIB9","doi-asserted-by":"crossref","unstructured":"Holodniy M , Kim S , Katzenstein D , Konrad M , Groves E & Merigan TC (1991) Inhibition of human immunodeficiency virus gene amplification by heparin. J Clin Microbiol 29: 676\u00e2\u0080\u0093679.","DOI":"10.1128\/JCM.29.4.676-679.1991"},{"key":"10.1111\/j.1574-695X.2006.00191.x-BIB10","doi-asserted-by":"crossref","unstructured":"Holodniy M , Mole L , Yen-Lieberman B , Margolis D , Starkey C , Carroll R , Spahlinger T , Todd J & Jackson JB (1995) Comparative stabilities of quantitative human immunodeficiency virus RNA in plasma from samples collected in VACUTAINER CPT, VACUTAINER PPT, and standard VACUTAINER tubes. J Clin Microbiol 33: 1562\u00e2\u0080\u00931566.","DOI":"10.1128\/JCM.33.6.1562-1566.1995"},{"key":"10.1111\/j.1574-695X.2006.00191.x-BIB14","volume-title":"Nucleic Acid Testing for Human Disease","author":"Krajden","year":"2005"},{"key":"10.1111\/j.1574-695X.2006.00191.x-BIB12","doi-asserted-by":"crossref","unstructured":"Krajden M , Comanor L , Rifkin O , Grigoriew A , Minor JM & Kapke GF (1998) Assessment of Hepatitis B virus DNA stability in serum by the Chiron quantiplex branched-DNA assay. J Clin Microbiol 36: 382\u00e2\u0080\u0093386.","DOI":"10.1128\/JCM.36.2.382-386.1998"},{"key":"10.1111\/j.1574-695X.2006.00191.x-BIB13","unstructured":"Krajden M , Minor JM , Rifkin O & Comanor L (1999) Effect of multiple freeze\u00e2\u0080\u0093thaw cycles on Hepatitis B virus DNA and Hepatitis C virus RNA quantification as measured with branched-DNA technology. J Clin Microbiol 37: 1683\u00e2\u0080\u00931686."},{"key":"10.1111\/j.1574-695X.2006.00191.x-BIB15","unstructured":"Lanciotti RS , Jerst AJ , Nasci RS et al. (2000) Rapid detection of West Nile virus from human clinical specimens, filed-collected mosquitoes, and avian samples by a Taqman reverse-transcriptase PCR assay. J Clin Microbiol 38: 4066\u00e2\u0080\u00934071."},{"key":"10.1111\/j.1574-695X.2006.00191.x-BIB16","doi-asserted-by":"crossref","unstructured":"Lee DH , Li L , Andrus L & Prince AM (2002a) Stabilized viral nucleic acids in plasma as an alternative shipping method for NAT. Transfusion 42: 409\u00e2\u0080\u0093413.","DOI":"10.1046\/j.1525-1438.2002.00068.x"},{"key":"10.1111\/j.1574-695X.2006.00191.x-BIB17","doi-asserted-by":"crossref","unstructured":"Lee SS , Heathcote EJ , Reddy KR et al. (2002b) Prognostic factors and early predictability of sustained viral response with peginterferon alfa-2a (40KD). J Hepatol 37: 500\u00e2\u0080\u0093506.","DOI":"10.1016\/S0168-8278(02)00211-8"},{"key":"10.1111\/j.1574-695X.2006.00191.x-BIB18","doi-asserted-by":"crossref","unstructured":"Lefrere JJ , Roudot-Thoraval F , Lunel F et al. (2004) Expertise of French laboratories in detection, genotyping and quantification of Hepatitis C virus RNA is serum. J Clin Microbiol 42: 2027\u00e2\u0080\u00932030.","DOI":"10.1128\/JCM.42.5.2027-2030.2004"},{"key":"10.1111\/j.1574-695X.2006.00191.x-BIB20","doi-asserted-by":"crossref","unstructured":"Liu H , Rodes B , Chen CY & Steiner B (2001) New tests for syphilis: rational design of a PCR method for detection of Treponema pallidum in clinical specimens using unique regions of the DNA polymerase I gene. J Clin Microbiol 39: 1941\u00e2\u0080\u00931946.","DOI":"10.1128\/JCM.39.5.1941-1946.2001"},{"key":"10.1111\/j.1574-695X.2006.00191.x-BIB21","unstructured":"Lukehart SA , Godornes C , Molini BJ et al. (2004) Macrolide resistance in Treponema pallidum in the United States and Ireland. N Engl J Med 351: 154\u00e2\u0080\u0093158."},{"key":"10.1111\/j.1574-695X.2006.00191.x-BIB22","unstructured":"Mayo DR & Beckwith WH (2002) Inactivation of West Nile virus during serological testing and transport. J Clin Microbiol 40: 3044\u00e2\u0080\u00933046."},{"key":"10.1111\/j.1574-695X.2006.00191.x-BIB23","unstructured":"McNabb A , Chen R , Lee M , McIntyre L , Fung J & Isaac-Renton JL (2002) A two year surveillance of Norwalk-like virus gastroenteritis outbreaks in British Columbia. CACMID, Halifax, Nova Scotia, November, 2002."},{"key":"10.1111\/j.1574-695X.2006.00191.x-BIB25","unstructured":"Morshed MG , Fernando K , Simpson Y , Jones H , Byrne S , Rekart M , Champagne S , Noguchi M , Brunham R & Isaac-Renton J (2001) Polymerase Chain Reaction based (polA Gene) testing is more sensitive than Dark Field Microscopy (DFM) for detecting Treponema pallidum Genital Ulcer Disease (GUD). International Congress of Sexually Transmitted Infection. 2001 June 24\u00e2\u0080\u009327. Berlin, Germany."},{"key":"10.1111\/j.1574-695X.2006.00191.x-BIB24","doi-asserted-by":"crossref","unstructured":"Morshed MG , Scott JD , Fernando K , Geddes G , McNabb A , Mak S & Durden LA (2006) Distribution and characterization of Borrelia burgdorferi isolates from Ixodes scapularis and presence in mammalian hosts in Ontario, Canada. J Med Entomol 43: 762\u00e2\u0080\u0093773.","DOI":"10.1603\/0022-2585(2006)43[762:DACOBB]2.0.CO;2"},{"key":"10.1111\/j.1574-695X.2006.00191.x-BIB26","doi-asserted-by":"crossref","unstructured":"Neumaier M , Braun A & Wagener C (1998) Fundamentals of quality assessment of molecular amplification methods in clinical diagnostics. International Federation of Clinical Chemistry Scientific Division Committee on Molecular Biology Techniques. Clin Chem 44: 12\u00e2\u0080\u009326.","DOI":"10.1093\/clinchem\/44.1.12"},{"key":"10.1111\/j.1574-695X.2006.00191.x-BIB27","unstructured":"Neumann AU , Lam NP , Dahari H , Gretch DR , Wiley TE , Layden TJ & Perelson AS (1998) Hepatitis C viral dynamics in vivo and the antiviral efficacy of interferon-alpha therapy. Science 282: 103\u00e2\u0080\u0093107."},{"key":"10.1111\/j.1574-695X.2006.00191.x-BIB28","unstructured":"Niesters HG , Krajden M , Cork L , de Medina M , Hill M , Fries E & Osterhaus AD (2000) A multicenter study evaluation of the digene hybird capture II signal amplification technique of detection of Hepatitis B virus DNA in serum samples and testing of EUROHEP standards. J Clin Microbiol 38: 2150\u00e2\u0080\u00932155."},{"key":"10.1111\/j.1574-695X.2006.00191.x-BIB29","unstructured":"Nkoghe D , Nnegue S , Leonard P , Moutschen M & Demonty J (2004) Identification of factors influencing the response of antiretroviral treatment. Med Mal Infect 34: 37\u00e2\u0080\u009341."},{"key":"10.1111\/j.1574-695X.2006.00191.x-BIB30","doi-asserted-by":"crossref","unstructured":"Nowak MA , Bonhoeffer S , Hill AM , Boehme R , Thomas HC & McDade H (1996) Viral dynamics in Hepatitis B virus infection. Proc Natl Acad Sci USA 93: 4398\u00e2\u0080\u00934402.","DOI":"10.1073\/pnas.93.9.4398"},{"key":"10.1111\/j.1574-695X.2006.00191.x-BIB31","doi-asserted-by":"crossref","unstructured":"Oiu CY , Ciesielski CA , Myers G et al. (1992) Molecular epidemiology of HIV transmission in a dental practice. Science 256: 1165\u00e2\u0080\u00931171.","DOI":"10.1126\/science.256.5060.1165"},{"key":"10.1111\/j.1574-695X.2006.00191.x-BIB32","doi-asserted-by":"crossref","unstructured":"Papin J F , Vahrson W & Dittmer DP (2004) SYBR Green-based real-time quantitative PCR assay for detection of West Nile virus circumvents false-negative results due to strain variability. J Clin Microbiol 42: 1511\u00e2\u0080\u00931518.","DOI":"10.1128\/JCM.42.4.1511-1518.2004"},{"key":"10.1111\/j.1574-695X.2006.00191.x-BIB19","unstructured":"Peruski LF Jr & Peruski AH (2003) Rapid diagnostic assay in the genomic biology era: detection and identification of infectious disease and biological weapon agents. BioTechniques 35: 840\u00e2\u0080\u0093846."},{"key":"10.1111\/j.1574-695X.2006.00191.x-BIB33","doi-asserted-by":"crossref","unstructured":"Pillay A , Liu H , Chen CY , Holloway B , Sturm AW , Steiner B & Morse SA (1998) Molecular subtyping of Treponema pallidum subspecies pallidum. Sex Transm Dis 25: 408\u00e2\u0080\u0093414.","DOI":"10.1097\/00007435-199809000-00004"},{"key":"10.1111\/j.1574-695X.2006.00191.x-BIB34","unstructured":"Rene P , Desouza E , Desai K & Robson HG (1998) Turn-around time (TAT) for detection of Mycobacterium tuberculosis: impact of Bactec 460 and polymerase chain reaction methods. Abstr Intersci Conf Antimicrob Agents Chemother Intersci Conf Antimicrob Agents Chemother. 1998 Sep 24\u00e2\u0080\u009327; 38:163 (abstract no. D-120)."},{"key":"10.1111\/j.1574-695X.2006.00191.x-BIB36","doi-asserted-by":"crossref","unstructured":"Richter SS (2002) Laboratory assays for diagnosis and management of Hepatitis C virus infection. J Clin Microbiol 40: 4407\u00e2\u0080\u00934412.","DOI":"10.1128\/JCM.40.12.4407-4412.2002"},{"key":"10.1111\/j.1574-695X.2006.00191.x-BIB35","doi-asserted-by":"crossref","unstructured":"Rolph HJ , Lennon A , Riggio MP , Saunders WP , MacKenzie D , Coldero L & Bagg J (2001) Molecular identification of microorganisms from endodontic infections. J Clin Microbiol 39: 3282\u00e2\u0080\u00933289.","DOI":"10.1128\/JCM.39.9.3282-3289.2001"},{"key":"10.1111\/j.1574-695X.2006.00191.x-BIB37","unstructured":"Shi P-Y , Kauffman EB , Ren P et al. (2001) High-throughput detection of West Nile virus RNA. J Clin Microbiol 39: 1264\u00e2\u0080\u00931271."},{"key":"10.1111\/j.1574-695X.2006.00191.x-BIB38","doi-asserted-by":"crossref","unstructured":"Zaidi N , Konstantinou K & Zervos M (2003) The role of molecular biology and nucleic acid technology in the study of human infection and epidemiology. Arch Pathol Lab Med 127: 1098\u00e2\u0080\u00931105.","DOI":"10.5858\/2003-127-1098-TROMBA"},{"key":"10.1111\/j.1574-695X.2006.00191.x-BIB39","doi-asserted-by":"crossref","unstructured":"Zanghellini F , Boppana SB , Emery VC , Griffiths PD & Pass RF (1999) Asymptomatic primary Cytomegalovirus infection: virologic and immunologic features. J Infect Dis 180: 702\u00e2\u0080\u0093707.","DOI":"10.1086\/314939"}],"container-title":["FEMS Immunology & Medical Microbiology"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/academic.oup.com\/femspd\/article-pdf\/49\/2\/184\/19169475\/49-2-184.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,5,10]],"date-time":"2023-05-10T14:12:53Z","timestamp":1683727973000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/femspd\/article-lookup\/doi\/10.1111\/j.1574-695X.2006.00191.x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2007,3]]},"references-count":38,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2007,3]]}},"URL":"https:\/\/doi.org\/10.1111\/j.1574-695x.2006.00191.x","relation":{},"ISSN":["0928-8244","1574-695X"],"issn-type":[{"value":"0928-8244","type":"print"},{"value":"1574-695X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2007,3]]}}}