{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,15]],"date-time":"2026-05-15T04:49:29Z","timestamp":1778820569969,"version":"3.51.4"},"reference-count":30,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2011,12,29]],"date-time":"2011-12-29T00:00:00Z","timestamp":1325116800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Cellular Molecular Medi"],"published-print":{"date-parts":[[2012,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The syntaxin 11 (STX11) gene is mutated in a proportion of patients with familial haemophagocytic lymphohistiocytosis (FHL) and exocytosis of cytotoxic granules is impaired in STX11\u2010deficient NK cells. However, the subcellular localization, regulation of expression and molecular function of STX11 in NK cells and other cytotoxic lymphocytes remain unknown. Here we demonstrate that STX11 expression is strictly controlled by several mechanisms in a cell\u2010type\u2010specific manner and that the enzymatic activity of the proteasome is required for STX11 expression in NK cells. In resting NKL cells, STX11 was localized in the cation\u2010dependent mannose\u20106\u2010phosphate receptor (CD\u2010M6PR)\u2010containing compartment, which was clearly distinct from cytotoxic granules or Rab27a\u2010expressing vesicles. These subcellular structures appeared to fuse at the contact area with NK\u2010sensitive target cells as demonstrated by partial colocalization of STX11 with perforin and Rab27a. Although STX11\u2010deficent allo\u2010specific cytotoxic T\u2010lymphocytes efficiently lysed target cells and released cytotoxic granules, they exhibited a significantly lower extent of spontaneous association of perforin with Rab27a as compared with STX11\u2010expressing T cells. Thus, our results suggest that STX11 promotes the fusion of Rab27a\u2010expressing vesicles with cytotoxic granules and reveal an additional level of complexity in the spatial\/temporal segregation of subcellular structures participating in the process of granule\u2010mediated cytotoxicity.<\/jats:p>","DOI":"10.1111\/j.1582-4934.2011.01280.x","type":"journal-article","created":{"date-parts":[[2011,2,22]],"date-time":"2011-02-22T18:28:58Z","timestamp":1298399338000},"page":"129-141","source":"Crossref","is-referenced-by-count":27,"title":["Syntaxin 11 marks a distinct intracellular compartment recruited to the immunological synapse of NK cells to colocalize with cytotoxic granules"],"prefix":"10.1111","volume":"16","author":[{"given":"Alena","family":"Dabrazhynetskaya","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jinxia","family":"Ma","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andre 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