{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T11:16:26Z","timestamp":1791112586889,"version":"4.1.0"},"reference-count":51,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2012,4,26]],"date-time":"2012-04-26T00:00:00Z","timestamp":1335398400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Cellular Molecular Medi"],"published-print":{"date-parts":[[2012,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>It remains elusive as to what bone marrow (BM) cell types infiltrate into injured and\/or diseased tissues and subsequently differentiate to assume the phenotype of residential cells, for example, neurons, cardiac myocytes, keratocytes, <jats:italic>etc<\/jats:italic>., to repair damaged tissue. Here, we examined the possibility of whether BM cell invasion <jats:italic>via<\/jats:italic> circulation into uninjured and injured corneas could assume a keratocyte phenotype, using chimeric mice generated by transplantation of enhanced green fluorescent protein (EGFP)<jats:sup>+<\/jats:sup> BM cells into keratocan null (<jats:italic>Kera<\/jats:italic><jats:sup>\u2212\/\u2212<\/jats:sup>) and lumican null (<jats:italic>Lum<\/jats:italic><jats:sup>\u2212\/<jats:italic>\u2212<\/jats:italic><\/jats:sup>) mice. EGFP<jats:sup>+<\/jats:sup> BM cells assumed dendritic cell morphology, but failed to synthesize corneal\u2010specific keratan sulfate proteoglycans, that is KS\u2010lumican and KS\u2010keratocan. In contrast, some EGFP<jats:sup>+<\/jats:sup> BM cells introduced by intrastromal transplantation assumed keratocyte phenotypes. Furthermore, BM cells were isolated from <jats:italic>Kera\u2010Cre\/ZEG<\/jats:italic> mice, a double transgenic mouse line in which cells expressing keratocan become EGFP<jats:sup>+<\/jats:sup> due to the synthesis of Cre driven by keratocan promoter. Three days after corneal and conjunctival transplantations of such BM cells into <jats:italic>Kera<\/jats:italic><jats:sup>\u2212\/\u2212<\/jats:sup> mice, green keratocan positive cells were found in the cornea, but not in conjunctiva. It is worthy to note that transplanted BM cells were rejected in 4 weeks. MSC isolated from BM were used to examine if BM mesenchymal stem cells (BM\u2010MSC) could assume keratocyte phenotype. When BM\u2010MSC were intrastromal\u2010transplanted into <jats:italic>Kera<\/jats:italic><jats:sup>\u2212\/\u2212<\/jats:sup> mice, they survived in the cornea without any immune and inflammatory responses and expressed keratocan in <jats:italic>Kera<\/jats:italic><jats:sup>\u2212\/\u2212<\/jats:sup> mice. These observations suggest that corneal intrastromal transplantation of BM\u2010MSC may be an effective treatment regimen for corneal diseases involving dysfunction of keratocytes.<\/jats:p>","DOI":"10.1111\/j.1582-4934.2011.01418.x","type":"journal-article","created":{"date-parts":[[2011,9,1]],"date-time":"2011-09-01T02:54:05Z","timestamp":1314845645000},"page":"1114-1124","source":"Crossref","is-referenced-by-count":84,"title":["Bone marrow mesenchymal stem cells can differentiate and assume corneal keratocyte phenotype"],"prefix":"10.1111","volume":"16","author":[{"given":"Hongshan","family":"Liu","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jianhua","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chia\u2010Yang","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yasuhito","family":"Hayashi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Winston W.\u2010Y.","family":"Kao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2012,4,26]]},"reference":[{"key":"e_1_2_7_2_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00689554"},{"key":"e_1_2_7_3_2","doi-asserted-by":"publisher","DOI":"10.1002\/jlb.55.4.423"},{"key":"e_1_2_7_4_2","doi-asserted-by":"crossref","first-page":"1638","DOI":"10.4049\/jimmunol.156.4.1638","article-title":"HIV\u20101\u2010Tat modulates the function of monocytes and alters their interactions with microvessel endothelial cells. 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