{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,3]],"date-time":"2026-09-03T17:58:07Z","timestamp":1788458287265,"version":"build-2803163510"},"reference-count":33,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2012,6,28]],"date-time":"2012-06-28T00:00:00Z","timestamp":1340841600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Cellular Molecular Medi"],"published-print":{"date-parts":[[2012,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Mucin glycoproteins are major secreted or membrane\u2010bound molecules that, in cancer, show modifications in both the mucin proteins expression and in the O\u2010glycosylation profile, generating some of the most relevant tumour markers in clinical use for decades. Thus far, the identification of these biomarkers has been based on the detection of either the protein or the O\u2010glycan modifications. We therefore aimed to identify the combined mucin and O\u2010glycan features, that is, specific glycoforms, in an attempt to increase specificity of these cancer biomarkers. Using in situ proximity ligation assays (PLA) based on existing monoclonal antibodies directed to MUC1, MUC2, MUC5AC and MUC6 mucins and to cancer\u2010associated carbohydrate antigens Tn, Sialyl\u2010Tn (STn), T, Sialyl\u2010Le<jats:sup>a<\/jats:sup> (SLe<jats:sup>a<\/jats:sup>) and Sialyl\u2010Le<jats:sup>x<\/jats:sup> (SLe<jats:sup>x<\/jats:sup>) we screened a series of 28 mucinous adenocarcinomas from different locations (stomach, ampulla of Vater, colon, lung, breast and ovary) to detect specific mucin glycoforms. We detected Tn\/STn\/SLe<jats:sup>a<\/jats:sup>\/SLe<jats:sup>x<\/jats:sup>\u2010MUC1 and STn\/SLe<jats:sup>a<\/jats:sup>\/SLe<jats:sup>x<\/jats:sup>\u2010MUC2 glycoforms in $50% of the cases, with a variable distribution among organs. Some new glycoforms\u2010T\/SLe<jats:sup>a<\/jats:sup>\u2010MUC2, STn\/T\/SLe<jats:sup>a<\/jats:sup>\/SLe<jats:sup>x<\/jats:sup>\u2010MUC5AC and STn\/T\/SLe<jats:sup>a<\/jats:sup>\/SLe<jats:sup>x<\/jats:sup>\u2010MUC6\u2010were identified for the first time in the present study in a variable percentage of cases from different organs. In conclusion, application of the PLA technique allowed sensitive detection of specific aberrant mucin glycoforms in cancer, increasing specificity to the use of antibodies either to the mucin protein backbone or to the O\u2010glycan haptens alone.<\/jats:p>","DOI":"10.1111\/j.1582-4934.2011.01436.x","type":"journal-article","created":{"date-parts":[[2011,9,1]],"date-time":"2011-09-01T04:55:52Z","timestamp":1314852952000},"page":"1474-1484","source":"Crossref","is-referenced-by-count":70,"title":["Identification of new cancer biomarkers based on aberrant mucin glycoforms by <i>in situ<\/i> proximity ligation"],"prefix":"10.1111","volume":"16","author":[{"given":"Rita","family":"Pinto","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ana S.","family":"Carvalho","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tim","family":"Conze","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ana","family":"Magalh\u00e3es","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gianfranco","family":"Picco","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joy M.","family":"Burchell","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joyce","family":"Taylor\u2010Papadimitriou","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Celso A.","family":"Reis","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Raquel","family":"Almeida","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ulla","family":"Mandel","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Henrik","family":"Clausen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ola","family":"S\u00f6derberg","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Leonor","family":"David","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2012,6,28]]},"reference":[{"key":"e_1_2_7_2_2","doi-asserted-by":"publisher","DOI":"10.1136\/jcp.2009.071035"},{"key":"e_1_2_7_3_2","doi-asserted-by":"publisher","DOI":"10.1093\/glycob\/cwj044"},{"key":"e_1_2_7_4_2","first-page":"5476","article-title":"Development and characterization of breast cancer reactive monoclonal antibodies directed to the core protein of the human milk mucin","volume":"47","author":"Burchell J","year":"1987","journal-title":"Cancer Res"},{"key":"e_1_2_7_5_2","doi-asserted-by":"publisher","DOI":"10.1023\/A:1006939432665"},{"key":"e_1_2_7_6_2","doi-asserted-by":"publisher","DOI":"10.1021\/pr1005229"},{"key":"e_1_2_7_7_2","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-09-2893"},{"key":"e_1_2_7_8_2","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth947"},{"key":"e_1_2_7_9_2","doi-asserted-by":"publisher","DOI":"10.1586\/epr.10.10"},{"key":"e_1_2_7_10_2","doi-asserted-by":"publisher","DOI":"10.1093\/glycob\/cwp161"},{"key":"e_1_2_7_11_2","unstructured":"EdgeSB ByrdDR ComptonCC et al. editors.AJCC Cancer staging manual.7th ed. 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