{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T06:09:11Z","timestamp":1792130951451,"version":"4.4.0"},"reference-count":43,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2012,8,23]],"date-time":"2012-08-23T00:00:00Z","timestamp":1345680000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Cellular Molecular Medi"],"published-print":{"date-parts":[[2012,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Cardiac fibrosis after myocardial infarction (<jats:styled-content style=\"fixed-case\">MI<\/jats:styled-content>) has been identified as a key factor in the development of heart failure. Although dysregulation of <jats:styled-content style=\"fixed-case\">microRNA (miRNA)<\/jats:styled-content> is involved in various pathophysiological processes in the heart, the role of <jats:styled-content style=\"fixed-case\">miRNA<\/jats:styled-content> in fibrosis regulation after <jats:styled-content style=\"fixed-case\">MI<\/jats:styled-content> is not clear. Previously we observed the correlation between fibrosis and the miR\u201024 expression in hypertrophic hearts, herein we assessed how miR\u201024 regulates fibrosis after <jats:styled-content style=\"fixed-case\">MI<\/jats:styled-content>. Using <jats:styled-content style=\"fixed-case\">qRT<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content>, we showed that miR\u201024 was down\u2010regulated in the <jats:styled-content style=\"fixed-case\">MI<\/jats:styled-content> heart; the change in miR\u201024 expression was closely related to extracellular matrix (<jats:styled-content style=\"fixed-case\">ECM<\/jats:styled-content>) remodelling. <jats:italic>In vivo<\/jats:italic>, miR\u201024 could improve heart function and attenuate fibrosis in the infarct border zone of the heart two\u00a0weeks after <jats:styled-content style=\"fixed-case\">MI<\/jats:styled-content> through intramyocardial injection of Lentiviruses. Moreover, <jats:italic>in vitro<\/jats:italic> experiments suggested that up\u2010regulation of miR\u201024 by synthetic miR\u201024 precursors could reduce fibrosis and also decrease the differentiation and migration of cardiac fibroblasts (<jats:styled-content style=\"fixed-case\">CFs<\/jats:styled-content>). <jats:styled-content style=\"fixed-case\">TGF<\/jats:styled-content>\u2010\u03b2 (a pathological mediator of fibrotic disease) increased miR\u201024 expression, overexpression of miR\u201024 reduced <jats:styled-content style=\"fixed-case\">TGF<\/jats:styled-content>\u2010\u03b2 secretion and Smad2\/3 phosphorylation in <jats:styled-content style=\"fixed-case\">CFs<\/jats:styled-content>. By performing microarray analyses and bioinformatics analyses, we found furin to be a potential target for miR\u201024 in fibrosis (furin is a protease which controls latent <jats:styled-content style=\"fixed-case\">TGF<\/jats:styled-content>\u2010\u03b2 activation processing). Finally, we demonstrated that protein and <jats:styled-content style=\"fixed-case\">mRNA<\/jats:styled-content> levels of furin were regulated by miR\u201024 in <jats:styled-content style=\"fixed-case\">CFs<\/jats:styled-content>. These findings suggest that miR\u201024 has a critical role in <jats:styled-content style=\"fixed-case\">CF<\/jats:styled-content> function and cardiac fibrosis after <jats:styled-content style=\"fixed-case\">MI<\/jats:styled-content> through a furin\u2013<jats:styled-content style=\"fixed-case\">TGF<\/jats:styled-content>\u2010\u03b2 pathway. Thus, miR\u201024 may be used as a target for treatment of <jats:styled-content style=\"fixed-case\">MI<\/jats:styled-content> and other fibrotic heart diseases.<\/jats:p>","DOI":"10.1111\/j.1582-4934.2012.01523.x","type":"journal-article","created":{"date-parts":[[2012,1,20]],"date-time":"2012-01-20T09:24:40Z","timestamp":1327051480000},"page":"2150-2160","source":"Crossref","is-referenced-by-count":249,"title":["Micro<scp>RNA<\/scp>\u201024 regulates cardiac fibrosis after myocardial infarction"],"prefix":"10.1111","volume":"16","author":[{"given":"Jue","family":"Wang","sequence":"first","affiliation":[{"name":"State Key Laboratory of Translational Cardiovascular Medicine Fuwai Hospital &amp; Cardiovascular Institute Chinese Academy of Medical Sciences Peking Union Medical College Beijing China"},{"name":"Department of Surgery Fuwai Hospital &amp; Cardiovascular Institute Chinese Academy of Medical Sciences Peking Union Medical College Beijing China"},{"name":"Research Center for Cardiac Regenerative Medicine Ministry of Health Beijing China"},{"name":"Department of Thoracic and Cardiovascular Surgery The First Affiliated Hospital of Wenzhou Medical College Wenzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Weicong","family":"Huang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Translational Cardiovascular Medicine Fuwai Hospital &amp; Cardiovascular Institute Chinese Academy of Medical Sciences Peking Union Medical College Beijing China"},{"name":"Department of Thoracic and Cardiovascular Surgery The First Affiliated Hospital of Wenzhou Medical College Wenzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ruixia","family":"Xu","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Translational Cardiovascular Medicine Fuwai Hospital &amp; Cardiovascular Institute Chinese Academy of Medical Sciences Peking Union Medical College Beijing China"},{"name":"Department of Surgery Fuwai Hospital &amp; Cardiovascular Institute Chinese Academy of Medical Sciences Peking Union Medical College Beijing China"},{"name":"Research Center for Cardiac Regenerative Medicine Ministry of Health Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yu","family":"Nie","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Translational Cardiovascular Medicine Fuwai Hospital &amp; Cardiovascular Institute Chinese Academy of Medical Sciences Peking Union Medical College Beijing China"},{"name":"Department of Surgery Fuwai Hospital &amp; Cardiovascular Institute Chinese Academy of Medical Sciences Peking Union Medical College Beijing China"},{"name":"Research Center for Cardiac Regenerative Medicine Ministry of Health Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoqing","family":"Cao","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Translational Cardiovascular Medicine 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