{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T17:38:29Z","timestamp":1790271509266,"version":"4.1.0"},"reference-count":32,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2012,9,26]],"date-time":"2012-09-26T00:00:00Z","timestamp":1348617600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Cellular Molecular Medi"],"published-print":{"date-parts":[[2012,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>After a myocardial infarction, thinning and expansion of the fibrotic scar contribute to progressive heart failure. The loss of elastin is a major contributor to adverse extracellular matrix remodelling of the infarcted heart, and restoration of the elastic properties of the infarct region can prevent ventricular dysfunction. We implanted cells genetically modified to overexpress elastin to re\u2010establish the elastic properties of the infarcted myocardium and prevent cardiac failure. A full\u2010length human elastin <jats:styled-content style=\"fixed-case\">cDNA<\/jats:styled-content> was cloned, subcloned into an adenoviral vector and then transduced into rat bone marrow stromal cells (<jats:styled-content style=\"fixed-case\">BMSCs<\/jats:styled-content>). <jats:italic>In vitro<\/jats:italic> studies showed that BMSCs expressed the elastin protein, which was deposited into the extracellular matrix. Transduced BMSCs were injected into the infarcted myocardium of adult rats. Control groups received either BMSCs transduced with the green fluorescent protein gene or medium alone. Elastin deposition in the infarcted myocardium was associated with preservation of myocardial tissue structural integrity (by birefringence of polarized light; <jats:italic>P<\/jats:italic>\u00a0&lt;\u00a00.05 <jats:italic>versus<\/jats:italic> controls). As a result, infarct scar thickness and diastolic compliance were maintained and infarct expansion was prevented (<jats:italic>P<\/jats:italic>\u00a0&lt;\u00a00.05 <jats:italic>versus<\/jats:italic> controls). Over a 9\u2010week period, rats implanted with BMSCs demonstrated better cardiac function than medium controls; however, rats receiving BMSCs overexpressing elastin showed the greatest functional improvement (<jats:italic>P<\/jats:italic>\u00a0&lt;\u00a00.01). Overexpression of elastin in the infarcted heart preserved the elastic structure of the extracellular matrix, which, in turn, preserved diastolic function, prevented ventricular dilation and preserved cardiac function. This cell\u2010based gene therapy provides a new approach to cardiac regeneration.<\/jats:p>","DOI":"10.1111\/j.1582-4934.2012.01560.x","type":"journal-article","created":{"date-parts":[[2012,3,22]],"date-time":"2012-03-22T22:59:20Z","timestamp":1332457160000},"page":"2429-2439","source":"Crossref","is-referenced-by-count":36,"title":["Elastin overexpression by cell\u2010based gene therapy preserves matrix and prevents cardiac dilation"],"prefix":"10.1111","volume":"16","author":[{"given":"Shu\u2010Hong","family":"Li","sequence":"first","affiliation":[{"name":"Division of Cardiovascular Surgery and Toronto General Research Institute University Health Network and Department of Surgery Division of Cardiac Surgery University of Toronto Toronto ON Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhuo","family":"Sun","sequence":"additional","affiliation":[{"name":"Division of Cardiovascular Surgery and Toronto General Research Institute University Health Network and Department of Surgery Division of Cardiac Surgery University of Toronto Toronto ON Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lily","family":"Guo","sequence":"additional","affiliation":[{"name":"Division of Cardiovascular Surgery and Toronto General Research Institute University Health Network and Department of Surgery Division of Cardiac Surgery University of Toronto Toronto ON Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mihan","family":"Han","sequence":"additional","affiliation":[{"name":"Division of Cardiovascular Surgery and Toronto General Research Institute University Health Network and Department of Surgery Division of Cardiac Surgery University of Toronto Toronto ON Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael F. G.","family":"Wood","sequence":"additional","affiliation":[{"name":"Division of Biophysics and Bioimaging Ontario Cancer Institute\/University Health Network and Departments of Medical Biophysics and Radiation Oncology University of Toronto Toronto ON Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nirmalya","family":"Ghosh","sequence":"additional","affiliation":[{"name":"Division of Biophysics and Bioimaging Ontario Cancer Institute\/University Health Network and Departments of Medical Biophysics and Radiation Oncology University of Toronto Toronto ON Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"I.","family":"Alex Vitkin","sequence":"additional","affiliation":[{"name":"Division of Biophysics and Bioimaging Ontario Cancer Institute\/University Health Network and Departments of Medical Biophysics and Radiation Oncology University of Toronto Toronto ON Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Richard D.","family":"Weisel","sequence":"additional","affiliation":[{"name":"Division of Cardiovascular Surgery and Toronto General Research Institute University Health Network and Department of Surgery Division of Cardiac Surgery University of Toronto Toronto ON Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ren\u2010Ke","family":"Li","sequence":"additional","affiliation":[{"name":"Division of Cardiovascular Surgery and Toronto General Research Institute University Health Network and Department of Surgery Division of Cardiac Surgery University of Toronto Toronto ON Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2012,9,26]]},"reference":[{"key":"e_1_2_7_2_1","first-page":"H1406","article-title":"Progressive ventricular remodeling in rat with myocardial infarction","volume":"260","author":"Pfeffer JM","year":"1991","journal-title":"Am J 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