{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T20:36:29Z","timestamp":1792182989374,"version":"4.4.0"},"reference-count":40,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2012,12,13]],"date-time":"2012-12-13T00:00:00Z","timestamp":1355356800000},"content-version":"vor","delay-in-days":12,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Cellular Molecular Medi"],"published-print":{"date-parts":[[2012,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Human immunodeficiency virus type 1 (<jats:styled-content style=\"fixed-case\">HIV<\/jats:styled-content>\u20101) infection of the central nervous system (<jats:styled-content style=\"fixed-case\">CNS<\/jats:styled-content>) affects cross\u2010talk between the individual cell types of the neurovascular unit, which then contributes to disruption of the blood\u2013brain barrier (<jats:styled-content style=\"fixed-case\">BBB<\/jats:styled-content>) and the development of neurological dysfunctions. Although the toxicity of <jats:styled-content style=\"fixed-case\">HIV<\/jats:styled-content>\u20101 on neurons, astrocytes and brain endothelial cells has been widely studied, there are no reports addressing the influence of <jats:styled-content style=\"fixed-case\">HIV<\/jats:styled-content>\u20101 on pericytes. Therefore, the purpose of this study was to evaluate whether or not pericytes can be infected with <jats:styled-content style=\"fixed-case\">HIV<\/jats:styled-content>\u20101 and how such an infection affects the barrier function of brain endothelial cells. Our results indicate that human brain pericytes express the major <jats:styled-content style=\"fixed-case\">HIV<\/jats:styled-content>\u20101 receptor <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>4 and co\u2010receptors <jats:styled-content style=\"fixed-case\">CXCR<\/jats:styled-content>4 and <jats:styled-content style=\"fixed-case\">CCR<\/jats:styled-content>5. We also determined that <jats:styled-content style=\"fixed-case\">HIV<\/jats:styled-content>\u20101 can replicate, although at a low level, in human brain pericytes as detected by <jats:styled-content style=\"fixed-case\">HIV<\/jats:styled-content>\u20101 p24 <jats:styled-content style=\"fixed-case\">ELISA<\/jats:styled-content>. Pericytes were susceptible to infection with both the X4\u2010tropic <jats:styled-content style=\"fixed-case\">NL<\/jats:styled-content>4\u20103 and R5\u2010tropic <jats:styled-content style=\"fixed-case\">JR<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">CSF HIV<\/jats:styled-content>\u20101 strains. Moreover, <jats:styled-content style=\"fixed-case\">HIV<\/jats:styled-content>\u20101 infection of pericytes resulted in compromised integrity of an <jats:italic>in vitro<\/jats:italic> model of the <jats:styled-content style=\"fixed-case\">BBB<\/jats:styled-content>. These findings indicate that human brain pericytes can be infected with <jats:styled-content style=\"fixed-case\">HIV<\/jats:styled-content>\u20101 and suggest that infected pericytes are involved in the progression of <jats:styled-content style=\"fixed-case\">HIV<\/jats:styled-content>\u20101\u2010induced <jats:styled-content style=\"fixed-case\">CNS<\/jats:styled-content> damage.<\/jats:p>","DOI":"10.1111\/j.1582-4934.2012.01622.x","type":"journal-article","created":{"date-parts":[[2012,9,4]],"date-time":"2012-09-04T15:47:18Z","timestamp":1346773638000},"page":"2950-2957","source":"Crossref","is-referenced-by-count":112,"title":["Infection of human pericytes by <scp>HIV<\/scp>\u20101 disrupts the integrity of the blood\u2013brain barrier"],"prefix":"10.1111","volume":"16","author":[{"given":"Shinsuke","family":"Nakagawa","sequence":"first","affiliation":[{"name":"Molecular Neuroscience and Vascular Biology Laboratory Department of Neurosurgery University of Kentucky Medical Center 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