{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T05:41:44Z","timestamp":1790228504437,"version":"4.1.0"},"reference-count":37,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2012,12,13]],"date-time":"2012-12-13T00:00:00Z","timestamp":1355356800000},"content-version":"vor","delay-in-days":12,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Cellular Molecular Medi"],"published-print":{"date-parts":[[2012,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>In the labouring uterus, millions of myocytes forming the complex geometrical structure of myometrium contract in synchrony to increase intrauterine pressure, dilate the cervix and eventually expel the foetus through the birth canal. The mechanisms underlying the precise coordination of contractions in human myometrium are not completely understood. In the present study, we have characterized the spatio\u2010temporal properties of tissue\u2010level [<jats:styled-content style=\"fixed-case\"><jats:roman>Ca<\/jats:roman><\/jats:styled-content><jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub> transients in thin slices of intact human myometrium. We found that the waveform of [<jats:styled-content style=\"fixed-case\"><jats:roman>Ca<\/jats:roman><\/jats:styled-content><jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub> transients and isotonic contractions recorded from thin slices was similar to the waveform of isometric contractions recorded from the larger strips in traditional organ bath experiments, suggesting that the spatio\u2010temporal information obtained from thin slices is representative of the whole tissue. By comparing the time course of [<jats:styled-content style=\"fixed-case\"><jats:roman>Ca<\/jats:roman><\/jats:styled-content><jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub> transients in individual cells to that recorded from the bundles of myocytes we found that the majority of myocytes produce rapidly propagating long\u2010lasting [<jats:styled-content style=\"fixed-case\"><jats:roman>Ca<\/jats:roman><\/jats:styled-content><jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub> transients accompanied by contractions. We also found a small number of cells showing desynchronized [<jats:styled-content style=\"fixed-case\"><jats:roman>Ca<\/jats:roman><\/jats:styled-content><jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub> oscillations that did not trigger contractions. The [<jats:styled-content style=\"fixed-case\"><jats:roman>Ca<\/jats:roman><\/jats:styled-content><jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub> oscillations in these cells were insensitive to nifedipine, but readily inhibited by the T\u2010type <jats:styled-content style=\"fixed-case\"><jats:roman>Ca<\/jats:roman><\/jats:styled-content><jats:sup>2+<\/jats:sup> channel inhibitor <jats:styled-content style=\"fixed-case\">NNC<\/jats:styled-content>55\u20100396. In conclusion, our data suggest that the spread of [<jats:styled-content style=\"fixed-case\"><jats:roman>Ca<\/jats:roman><\/jats:styled-content><jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub> signals in human myometrium is achieved <jats:italic>via<\/jats:italic> propagation of long\u2010lasting action potentials. The propagation was fast when action potentials propagated along bundles of myocytes and slower when propagating between the bundles of uterine myocytes.<\/jats:p>","DOI":"10.1111\/j.1582-4934.2012.01626.x","type":"journal-article","created":{"date-parts":[[2012,9,4]],"date-time":"2012-09-04T15:47:40Z","timestamp":1346773660000},"page":"2990-3000","source":"Crossref","is-referenced-by-count":19,"title":["Characterization of the tissue\u2010level <scp><scp>Ca<\/scp><\/scp><sup>2+<\/sup> signals in spontaneously contracting human myometrium"],"prefix":"10.1111","volume":"16","author":[{"given":"Gilles","family":"Bru\u2010Mercier","sequence":"first","affiliation":[{"name":"Warwick Medical School University of Warwick Coventry UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joanna E.","family":"Gullam","sequence":"additional","affiliation":[{"name":"Medical School University of Exeter Exeter 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