{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,8]],"date-time":"2026-01-08T04:18:25Z","timestamp":1767845905522,"version":"3.49.0"},"reference-count":35,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2012,12,13]],"date-time":"2012-12-13T00:00:00Z","timestamp":1355356800000},"content-version":"vor","delay-in-days":12,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Cellular Molecular Medi"],"published-print":{"date-parts":[[2012,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Small\u2010conductance calcium\u2010activated potassium (<jats:styled-content style=\"fixed-case\">SK<\/jats:styled-content>3) channels have been detected in human myometrium and we have previously shown a functional role of <jats:styled-content style=\"fixed-case\">SK<\/jats:styled-content> channels in human myometrium <jats:italic>in vitro<\/jats:italic>. The aims of this study were to identify the precise localization of <jats:styled-content style=\"fixed-case\">SK<\/jats:styled-content>3 channels and to quantify <jats:styled-content style=\"fixed-case\">SK<\/jats:styled-content>3 m<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content> expression in myometrium from pregnant and non\u2010pregnant women. Myometrial biopsies were obtained from pregnant (<jats:italic>n<\/jats:italic>\u00a0=\u00a011) and non\u2010pregnant (<jats:italic>n<\/jats:italic>\u00a0=\u00a011) women. The expression of <jats:styled-content style=\"fixed-case\">SK<\/jats:styled-content>3 channels was assessed using immunohistochemistry and <jats:styled-content style=\"fixed-case\">SK<\/jats:styled-content>3 m<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content> was determined by q<jats:styled-content style=\"fixed-case\">RT<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content>. In non\u2010pregnant myometrium <jats:styled-content style=\"fixed-case\">SK<\/jats:styled-content>3 immunoreactivity was observed in <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>34 positive (<jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>34<jats:sup>+<\/jats:sup>) interstitial Cajal\u2010like cells (<jats:styled-content style=\"fixed-case\">ICLC<\/jats:styled-content>), now called telocytes. Although <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>34<jats:sup>+<\/jats:sup> cells were also present in pregnant myometrium, they lacked <jats:styled-content style=\"fixed-case\">SK<\/jats:styled-content>3 immunoreactivity. Furthermore, the immunohistochemical results showed that <jats:styled-content style=\"fixed-case\">SK<\/jats:styled-content>3 expression in vascular endothelium was similar between the two groups. <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>117 immunoreactivity was only detected in small round cells that resemble mast cells. Compared to non\u2010pregnant myometrium we found significantly less <jats:styled-content style=\"fixed-case\">SK<\/jats:styled-content>3 m<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content> in pregnant myometrium. We demonstrate that <jats:styled-content style=\"fixed-case\">SK<\/jats:styled-content>3 channels are localized solely in <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>34<jats:sup>+<\/jats:sup> cells and not in smooth muscle cells, and that the molecular expression of <jats:styled-content style=\"fixed-case\">SK<\/jats:styled-content>3 channels is higher in non\u2010pregnant compared to pregnant myometrium. On the basis of our previous study and the present findings, we propose that <jats:styled-content style=\"fixed-case\">SK<\/jats:styled-content>3 activators reduce contractility in human myometrium by modulating telocyte function. This is the first report to provide evidence for a possible role of <jats:styled-content style=\"fixed-case\">SK<\/jats:styled-content>3 channels in human uterine telocytes.<\/jats:p>","DOI":"10.1111\/j.1582-4934.2012.01627.x","type":"journal-article","created":{"date-parts":[[2012,9,4]],"date-time":"2012-09-04T15:47:40Z","timestamp":1346773660000},"page":"3001-3008","source":"Crossref","is-referenced-by-count":44,"title":["Immunolocalization and expression of small\u2010conductance calcium\u2010activated potassium channels in human myometrium"],"prefix":"10.1111","volume":"16","author":[{"given":"Sofia T.","family":"Rosenbaum","sequence":"first","affiliation":[{"name":"Department of Gynaecology and Obstetrics Holbaek Hospital Holbaek Denmark"},{"name":"Smooth Muscle Research Center Department of Clinical Biochemistry Koege Hospital Holbaek Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Julie","family":"Sval\u00f8","sequence":"additional","affiliation":[{"name":"Smooth Muscle Research Center Department of Clinical Biochemistry Koege Hospital Holbaek Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Karsten","family":"Nielsen","sequence":"additional","affiliation":[{"name":"Department of Clinical Pathology Naestved Hospital Naestved Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Torben","family":"Larsen","sequence":"additional","affiliation":[{"name":"Department of Gynaecology and Obstetrics Holbaek Hospital Holbaek Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J\u00f8rgen C.","family":"J\u00f8rgensen","sequence":"additional","affiliation":[{"name":"Department of Gynaecology and Obstetrics Holbaek Hospital Holbaek 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