{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,11]],"date-time":"2026-08-11T03:51:14Z","timestamp":1786420274030,"version":"build-2736575974"},"reference-count":31,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2012,12,13]],"date-time":"2012-12-13T00:00:00Z","timestamp":1355356800000},"content-version":"vor","delay-in-days":12,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Cellular Molecular Medi"],"published-print":{"date-parts":[[2012,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Persistent high\u2010risk human papillomavirus (<jats:styled-content style=\"fixed-case\">HPV<\/jats:styled-content>) infection is strongly associated with the development of high\u2010grade cervical intraepithelial neoplasia or cancer (<jats:styled-content style=\"fixed-case\">CIN<\/jats:styled-content>3+). However, <jats:styled-content style=\"fixed-case\">HPV<\/jats:styled-content> infection is common and usually transient. Viral load measured at a single time\u2010point is a poor predictor of the natural history of <jats:styled-content style=\"fixed-case\">HPV<\/jats:styled-content> infection. The profile of viral load evolution over time could distinguish <jats:styled-content style=\"fixed-case\">HPV<\/jats:styled-content> infections with carcinogenic potential from infections that regress. A case\u2010cohort natural history study was set\u2010up using a Belgian laboratory database processing more than 100,000 liquid cytology specimens annually. All cytology leftovers were submitted to real\u2010time <jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content> testing identifying E6\/E7 genes of 17 <jats:styled-content style=\"fixed-case\">HPV<\/jats:styled-content> types, with viral load expressed as <jats:styled-content style=\"fixed-case\">HPV<\/jats:styled-content> copies\/cell. Samples from untreated women who developed <jats:styled-content style=\"fixed-case\">CIN<\/jats:styled-content>3+ (<jats:italic>n<\/jats:italic>\u00a0=\u00a0138) and women with transient <jats:styled-content style=\"fixed-case\">HPV<\/jats:styled-content> infection (<jats:italic>n<\/jats:italic>\u00a0=\u00a0601) who contributed at least three viral load measurements were studied. Only single\u2010type <jats:styled-content style=\"fixed-case\">HPV<\/jats:styled-content> infections were selected. The changes in viral load over time were assessed by the linear regression slope for the productive and\/or clearing phase of infection in women developing <jats:styled-content style=\"fixed-case\">CIN<\/jats:styled-content>3+ and women with transient infection respectively. Transient <jats:styled-content style=\"fixed-case\">HPV<\/jats:styled-content> infections generated similar increasing (0.21 copies\/cell\/day) and decreasing (\u22120.28 copies\/cell\/day) viral load slopes. In <jats:styled-content style=\"fixed-case\">HPV<\/jats:styled-content> infections leading to <jats:styled-content style=\"fixed-case\">CIN<\/jats:styled-content>3+, the viral load increased almost linearly with a slope of 0.0028 copies\/cell\/day. Difference in slopes between transient infections and infections leading to <jats:styled-content style=\"fixed-case\">CIN<\/jats:styled-content>3+ was highly significant (<jats:italic>P\u00a0<\/jats:italic>&lt;\u00a0.0001). Serial type\u2010specific viral load measurements predict the natural history of <jats:styled-content style=\"fixed-case\">HPV<\/jats:styled-content> infections and could be used to triage women in <jats:styled-content style=\"fixed-case\">HPV<\/jats:styled-content>\u2010based cervical cancer screening.<\/jats:p>","DOI":"10.1111\/j.1582-4934.2012.01631.x","type":"journal-article","created":{"date-parts":[[2012,9,15]],"date-time":"2012-09-15T05:19:37Z","timestamp":1347686377000},"page":"3096-3104","source":"Crossref","is-referenced-by-count":56,"title":["Changes in type\u2010specific human papillomavirus load predict progression to cervical cancer"],"prefix":"10.1111","volume":"16","author":[{"given":"Christophe E.","family":"Depuydt","sequence":"first","affiliation":[{"name":"RIATOL, Department of Molecular Diagnostics Sonic Healthcare Benelux Antwerp Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Arnold M.","family":"Criel","sequence":"additional","affiliation":[{"name":"RIATOL, Department of Molecular Diagnostics Sonic Healthcare Benelux Antwerp Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ina H.","family":"Benoy","sequence":"additional","affiliation":[{"name":"RIATOL, Department of Molecular Diagnostics Sonic Healthcare Benelux Antwerp Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marc","family":"Arbyn","sequence":"additional","affiliation":[{"name":"Unit of Cancer Epidemiology Scientific Institute of Public Health Brussels Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Annie J.","family":"Vereecken","sequence":"additional","affiliation":[{"name":"RIATOL, Department of Molecular Diagnostics Sonic Healthcare Benelux Antwerp Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Johannes J.","family":"Bogers","sequence":"additional","affiliation":[{"name":"RIATOL, Department of Molecular Diagnostics Sonic Healthcare Benelux Antwerp Belgium"},{"name":"Laboratory for Cell Biology and Histology University of Antwerp Antwerp Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2012,12,13]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1136\/jcp.55.4.244"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1096-9896(199909)189:1<12::AID-PATH431>3.0.CO;2-F"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(98)12490-X"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrc2050"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/path.2192"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2006.05.117"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.21955"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1470-2045(09)70360-2"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.20480"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ygyno.2005.11.035"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1158\/1055-9965.EPI-09-0838"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.23012"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/jir049"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1086\/647993"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.10232"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcv.2005.09.001"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcv.2011.01.011"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1158\/1055-9965.EPI-09-0025"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1093\/jnci\/djn044"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1093\/jnci\/djq356"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1093\/jnci\/djr283"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-06-1057"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1086\/516784"},{"issue":"569","key":"e_1_2_7_25_1","first-page":"e1","article-title":"Vanden Broeck D, Ruymbeke MJ et al. 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