{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,6]],"date-time":"2026-03-06T14:21:54Z","timestamp":1772806914752,"version":"3.50.1"},"reference-count":22,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2012,5,15]],"date-time":"2012-05-15T00:00:00Z","timestamp":1337040000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["obgyn.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Acta Obstet Gynecol Scand"],"published-print":{"date-parts":[[2012,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p><jats:italic>Objective.<\/jats:italic> To examine the diagnostic accuracy of nucleic acid sequence based amplification (NASBA) and flow cytometry for E6&amp;7 human papillomavirus (HPV) mRNA detection in the triage of minor cytological abnormalities. <jats:italic>Design.<\/jats:italic> Prospective diagnostic accuracy study. <jats:italic>Setting.<\/jats:italic> Gynecology outpatient clinics of a university hospital. <jats:italic>Population.<\/jats:italic> 472 women with low\u2010grade squamous intraepithelial lesion (LSIL) or atypical squamous cells of undetermined significance (ASCUS). <jats:italic>Methods.<\/jats:italic> Residual material of the liquid\u2010based smears was tested by NASBA and by flow cytometry for E6&amp;E7 mRNA expression. Histological diagnosis was used as reference standard. <jats:italic>Main outcome measures.<\/jats:italic> Accuracy indices of the two techniques and of type 16\u2010specific NASBA for the detection of cervical intraepithelial neoplasia (CIN) 2+ and CIN3+, accuracy indices at age &gt;35 years, correlation between NASBA and flow, comparison between integrated and episomal high\u2010risk HPV infection for risk of CIN2+. <jats:italic>Results.<\/jats:italic> Both tests showed increased positivity rates with increasing severity of the lesion (<jats:italic>p<\/jats:italic> &lt; 0.05, chi\u2010squared test for trend). There was a positive correlation between NASBA and flow results (phi coefficient = 0.325). NASBA\u2010positive cases were more likely to have CIN2+ than were NASBA\u2010negative\/DNA\u2010positive for types 16, 18, 31, 33, 45 (25\/73 vs. 4\/52, <jats:italic>p<\/jats:italic>= 0.0004; Fisher\u2018s exact test). In the LSIL group the NASBA accuracy indices for CIN3+ were: sensitivity 75%, specificity 78.7% and positivity rate 20.8%, and for flow 77.8%, 64.5% and 35.9%, respectively. <jats:italic>Conclusions.<\/jats:italic> NASBA has favorable specificity and positivity rates for triaging LSIL prior to colposcopy. A relatively low sensitivity warrants cytological surveillance of the NASBA\u2010negative LSILs. Flow cytometry does not perform as well overall.<\/jats:p>","DOI":"10.1111\/j.1600-0412.2012.01414.x","type":"journal-article","created":{"date-parts":[[2012,4,6]],"date-time":"2012-04-06T18:26:45Z","timestamp":1333736805000},"page":"794-801","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":20,"title":["The diagnostic accuracy of two methods for E6&amp;7 mRNA detection in women with minor cytological abnormalities"],"prefix":"10.1111","volume":"91","author":[{"given":"GEORGE","family":"KOLIOPOULOS","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"CHARALAMBOS","family":"CHRELIAS","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"ASIMAKIS","family":"PAPPAS","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"SOFIA","family":"MAKRIDIMA","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"EMMANUEL","family":"KOUNTOURIS","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"MARIA","family":"ALEPAKI","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"ARIS","family":"SPATHIS","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"VICKY","family":"STATHOPOULOU","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"IOANNIS","family":"PANAYIOTIDES","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"PERICLES","family":"PANAGOPOULOS","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"PETROS","family":"KARAKITSOS","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"DIMITRIOS","family":"KASSANOS","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2012,5,15]]},"reference":[{"key":"e_1_2_8_2_2","doi-asserted-by":"crossref","first-page":"293","DOI":"10.1093\/jnci\/93.4.293","article-title":"ALTS Study group. 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