{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,10,16]],"date-time":"2023-10-16T08:46:08Z","timestamp":1697445968012},"reference-count":27,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2012,8,21]],"date-time":"2012-08-21T00:00:00Z","timestamp":1345507200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["obgyn.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Acta Obstet Gynecol Scand"],"published-print":{"date-parts":[[2012,10]]},"abstract":"<jats:title>Abstract\u2002<\/jats:title><jats:p><jats:italic>Objective.<\/jats:italic> To investigate the effects of chorionicity and severe twin to twin transfusion (TTTS) on maternal circulating cell\u2010free messenger RNA (cf\u2010mRNA). <jats:italic>Design<\/jats:italic>. Prospective cohort study. <jats:italic>Setting.<\/jats:italic> A UK tertiary referral Fetal Medicine Center. <jats:italic>Population<\/jats:italic>. All monochorionic (MC) twins complicated by severe TTTS (<jats:italic>n<\/jats:italic>= 23) and a cohort of uncomplicated dichorionic (DC) (<jats:italic>n<\/jats:italic>= 10) and MC (<jats:italic>n<\/jats:italic>= 7) pregnancies, between October 2006 and December 2007. <jats:italic>Methods<\/jats:italic>. Maternal cf\u2010mRNA encoding glyceraldehyde 3\u2010phosphate dehydrogenase (GAPDH), vascular endothelial growth factor receptor 1(VEGFR\u20101(Flt\u20101)), vascular endothelial growth factor A (VEGF\u2010A), Endoglin, placental growth factor (PlGF), tyrosine kinase with immunoglobulin\u2010like and EGF\u2010like domains 1 (Tie\u20101), angiopoietin 1 (Ang\u20101) and angiopoietin 2 (Ang\u20102) were measured by a quantitative two\u2010step real\u2010time PCR assay after extraction from maternal plasma. <jats:italic>Results<\/jats:italic>. The amounts of cf\u2010mRNA detectable are reported for uncomplicated DC, MC and TTTS pregnancies, respectively: GAPDH \u2013 80, 100 and 96%; VEGFR\u20101 \u2013 10, 0 and 26%; VEGF\u2010A\u2010 80, 71 and 96%; Endoglin\u201370, 71 and 91%; PlGF\u201370, 57, 26%; Tie\u20101 0, 43, 0%; Ang\u20101 71, 50 and 60% and Ang\u20102 83, 50 and 89%. There was a significant difference in VEGF\u2010A (medians DC \u2013337.3, MC \u2013 390.8, TTTS \u2013 618.6 copies\/mL plasma <jats:italic>p<\/jats:italic>= 0.024), Endoglin (medians DC\u201014.49, MC\u20101171, TTTS \u2013 2896 copies\/mL plasma <jats:italic>p<\/jats:italic>= 0.027) and Ang\u20102 (medians DC\u201013.66, MC\u20108.49, TTTS 44.80 copies\/mL plasma <jats:italic>p<\/jats:italic>= 0.007). <jats:italic>Conclusion<\/jats:italic>. Maternal cf\u2010mRNA could be reliably detected for GAPDH, PlGF, VEGF\u2010A, Endoglin, Ang\u20101 and Ang\u20102 in twin pregnancies and a significant difference was demonstrated in VEGF\u2010A, Endoglin and Ang\u20102 between uncomplicated twins and MC twin pregnancies complicated by TTTS. If such alterations in maternal cf\u2010mRNA precede the onset of clinically apparent disease, this may be used as an adjuvant blood test to complement ultrasound screening.<\/jats:p>","DOI":"10.1111\/j.1600-0412.2012.01507.x","type":"journal-article","created":{"date-parts":[[2012,8,3]],"date-time":"2012-08-03T17:31:41Z","timestamp":1344015101000},"page":"1206-1211","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Maternal cell\u2010free messenger RNA in twin pregnancies: the effects of chorionicity and severe twin to twin transfusion syndrome (TTTS)"],"prefix":"10.1111","volume":"91","author":[{"given":"CAROLINE E.","family":"FOX","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"AKIHIKO","family":"SEKIZAWA","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"SAMANTHA 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