{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,12]],"date-time":"2025-10-12T10:26:22Z","timestamp":1760264782781},"reference-count":26,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2004,9,2]],"date-time":"2004-09-02T00:00:00Z","timestamp":1094083200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Acta Ophthalmologica Scandinavica"],"published-print":{"date-parts":[[2004,10]]},"abstract":"<jats:title>Abstract.<\/jats:title><jats:p><jats:bold>Purpose:\u2002<\/jats:bold>  <jats:bold>Non\u2010obese diabetic (NOD) mice develop an autoimmune exocrinopathy characterized by hyposecretion of saliva and acinar cell atrophy. As the protein kinase C (PKC) system is involved in the signal transduction pathways associated with primary secretion and acinar cell differentiation and growth, the PKC profile was analysed in NOD mice.<\/jats:bold> <\/jats:p><jats:p><jats:bold>Methods:\u2002<\/jats:bold>  <jats:bold>Lacrimal glands from BALB\/c, NOD, NOD scid and transgenic NOD\u2003\u00d7\u2003interferon\u2010\u03b3 (IFN\u2010\u03b3) mice were analysed for their PKC profiles using antibodies against several conventional (\u03b1, \u03b2, \u03b3), novel (\u03b4, \u03b5, \u03b8) and atypical (\u03b9, \u03bb) PKC isoforms using the Streptavidin\/HRP (horseradish peroxidase) method.<\/jats:bold> <\/jats:p><jats:p><jats:bold>Results:\u2002<\/jats:bold>  <jats:bold>Acinar cells in BALB\/c control mice expressed two conventional (\u03b1, \u03b2) and two atypical (\u03b9, \u03bb) PKC isoforms. In NOD and transgenic NOD\u2003\u00d7\u2003IFN\u2010\u03b3 mice the same isoforms were more strongly expressed. NOD scid mice lacked all other PKC isoforms except PKC\u2003\u03bb.<\/jats:bold> <\/jats:p><jats:p><jats:bold>Conclusions:\u2002<\/jats:bold>  <jats:bold>Co\u2010expression of several PKC isoforms in single cell type may be necessary for transcriptional activation and agonist\u2010induced secretory responses. Hyposecretion in NOD mice was paradoxically associated with up\u2010regulation of the PKC system. This may be associated with a deranged signal transduction <jats:italic>per se<\/jats:italic> rather than with the immune\u2010inflammation, as the transgenic NOD\u2003\u00d7\u2003IFN\u2010\u03b3 mice showed similar PKC profiles. The NOD model does not reproduce lack\/consumption of PKC\u2003II and PKC as in Sj\u00f6gren's syndrome. This may be because the receptor autoantibodies in mice are directed against the adrenergic, not muscarinic, receptors. Lack and\/or low level PKC expression in NOD scid mouse may explain the excessive acinar cell apoptosis in this model.<\/jats:bold> <\/jats:p>","DOI":"10.1111\/j.1600-0420.2004.00310.x","type":"journal-article","created":{"date-parts":[[2004,9,28]],"date-time":"2004-09-28T15:41:43Z","timestamp":1096386103000},"page":"569-573","source":"Crossref","is-referenced-by-count":2,"title":["The protein kinase C system in focal adenitis of the lacrimal gland in the non\u2010obese diabetic mouse model for Sj\u00f6gren's syndrome"],"prefix":"10.1111","volume":"82","author":[{"given":"E\u2010K.","family":"Tensing","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J.","family":"T\u00f6rnwall","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"Hukkanen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"D. C. 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T.","family":"Konttinen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2004,9,28]]},"reference":[{"key":"e_1_2_6_2_1","first-page":"151","article-title":"Autoantibodies against lacrimal gland M3 muscarinic acetylcholine receptors in patients with primary Sj\u00f6gren's syndrome","volume":"39","author":"Bacman S","year":"1998","journal-title":"Invest Ophthalmol Vis Sci"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4615-5359-5_15"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/0024-3205(95)02196-P"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.152.11.5532"},{"key":"e_1_2_6_6_1","first-page":"607","article-title":"Functional changes in salivary glands of autoimmune\u2010prone NOD mice","volume":"236","author":"Hu Y","year":"1992","journal-title":"Am J Physiol"},{"key":"e_1_2_6_7_1","first-page":"433","article-title":"Down\u2010regulation of beta\u2010adrenergic receptors and signal transduction response in salivary glands of NOD mice","volume":"266","author":"Hu Y","year":"1994","journal-title":"Am J Physiol"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1042\/bj2910329"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0955-0674(96)80062-7"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1093\/emboj\/17.14.4046"},{"key":"e_1_2_6_11_1","first-page":"675","article-title":"Inappropriate apoptosis of salivary and lacrimal gland epithelium in immunodeficient NOD\u2010scid mice","volume":"16","author":"Kong L","year":"1998","journal-title":"Clin Exp Rheumatol"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1002\/art.1780350717"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/art.1780400515"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.95.13.7538"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.3109\/08916939809008035"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(88)90414-X"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1038\/346844a0"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4615-2417-5_4"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1006\/cimm.1996.0133"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006-2952(96)00308-5"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(05)61694-7"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.1002\/art.1780360309"},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.1006\/clin.1998.4598"},{"key":"e_1_2_6_24_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4615-5359-5_26"},{"key":"e_1_2_6_25_1","doi-asserted-by":"publisher","DOI":"10.1007\/s002329900226"},{"key":"e_1_2_6_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/0898-6568(92)90012-W"},{"key":"e_1_2_6_27_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.1993.264.4.C1045"}],"container-title":["Acta Ophthalmologica Scandinavica"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fj.1600-0420.2004.00310.x","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/j.1600-0420.2004.00310.x","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,13]],"date-time":"2023-10-13T08:36:15Z","timestamp":1697186175000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/j.1600-0420.2004.00310.x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2004,9,28]]},"references-count":26,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2004,10]]}},"alternative-id":["10.1111\/j.1600-0420.2004.00310.x"],"URL":"https:\/\/doi.org\/10.1111\/j.1600-0420.2004.00310.x","archive":["Portico"],"relation":{},"ISSN":["1395-3907","1600-0420"],"issn-type":[{"value":"1395-3907","type":"print"},{"value":"1600-0420","type":"electronic"}],"subject":[],"published":{"date-parts":[[2004,9,28]]}}}