{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,30]],"date-time":"2026-10-30T20:48:47Z","timestamp":1793393327441,"version":"4.5.0"},"reference-count":22,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2004,9,28]],"date-time":"2004-09-28T00:00:00Z","timestamp":1096329600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Acta Ophthalmologica Scandinavica"],"published-print":{"date-parts":[[2004,10]]},"abstract":"<jats:title>Abstract.<\/jats:title><jats:p><jats:bold>Purpose:\u2002<\/jats:bold>  <jats:bold>To study the change in expression of vascular endothelial growth factor (VEGF) and its receptor VEGFR\u20102 in the rabbit cornea and limbus following a penetrating, central corneal alkali burn. The influence of different cells on VEGF and VEGFR\u20102 expression was studied by excluding granulocytes from the wound area.<\/jats:bold> <\/jats:p><jats:p><jats:bold>Methods:\u2002<\/jats:bold>  <jats:bold>Fourteen New Zealand white rabbits were subjected to a penetrating, 5\u2010mm diameter, central corneal alkali burn in one eye under general anaesthesia. Seven of the rabbits were given injections of fucoidin for 36\u2003hours. The rabbits were killed after 36\u2003hours and the corneas were excised with a sclera rim and prepared for immunohistochemistry.<\/jats:bold> <\/jats:p><jats:p><jats:bold>Results:\u2002<\/jats:bold>  <jats:bold>Both VEGF and VEGFR\u20102 are strongly expressed in the frontline of repopulating epithelial, stromal and endothelial cells during wound healing, irrespective of granulocyte presence. Vascular endothelial cells express VEGF strongly after injury, but only in the presence of granulocytes.<\/jats:bold> <\/jats:p><jats:p><jats:bold>Conclusion:\u2002<\/jats:bold>  <jats:bold>Corneal neovascularization requires the presence of granulocytes to stimulate vascular endothelial cells. During wound healing in this area, VEGF is a factor that stimulates proliferation and migration and that is not influenced by granulocytes.<\/jats:bold> <\/jats:p>","DOI":"10.1111\/j.1600-0420.2004.00312.x","type":"journal-article","created":{"date-parts":[[2004,9,28]],"date-time":"2004-09-28T15:41:43Z","timestamp":1096386103000},"page":"557-563","source":"Crossref","is-referenced-by-count":115,"title":["Vascular endothelial growth factor (VEGF) and its receptor VEGFR\u20102 in the regulation of corneal neovascularization and wound healing"],"prefix":"10.1111","volume":"82","author":[{"given":"Lisha","family":"Gan","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Per","family":"Fagerholm","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jan","family":"Palmblad","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2004,9,28]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1755-3768.1987.tb07036.x"},{"key":"e_1_2_6_3_1","first-page":"1963","article-title":"Alkali burn\u2010induced synthesis of inflammatory eicosanoids in the rabbit corneal epithelium","volume":"38","author":"Cornners MS","year":"1997","journal-title":"Invest Ophthalmol Vis Sci"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/229560a0"},{"key":"e_1_2_6_5_1","first-page":"1029","article-title":"Vascular permeability factor\/vacular endothelial growth factor, microvascular hyperpermeability and angiogenesis","volume":"146","author":"Dvorak HF","year":"1995","journal-title":"Am J Pathol"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1034\/j.1600-0420.1999.770210.x"},{"key":"e_1_2_6_7_1","first-page":"4210","article-title":"Expression of VEGF and its receptor VEGF\u20102\/flk\u20101 following rabbit corneal alkali burn in the presence and absence of granulocytes. 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