{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,12]],"date-time":"2026-05-12T07:33:24Z","timestamp":1778571204514,"version":"3.51.4"},"reference-count":38,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2005,5,24]],"date-time":"2005-05-24T00:00:00Z","timestamp":1116892800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Acta Ophthalmologica Scandinavica"],"published-print":{"date-parts":[[2005,8]]},"abstract":"<jats:title>Abstract.<\/jats:title><jats:p><jats:bold>Purpose:\u2002<\/jats:bold>  <jats:bold>To investigate whether the expression of apoptosis\u2010related genes in normal conjunctival epithelial cells is age\u2010related (as a prerequisite to assessing whether dysregulation of apoptosis may be involved during degenerative diseases).<\/jats:bold> <\/jats:p><jats:p><jats:bold>Methods:\u2002<\/jats:bold>  <jats:bold>Differential expression of apoptosis\u2010related genes (e.g. apoptosis protease\u2010activating factor 1 [Apaf\u20101]; caspases [casp] 3, 5, 8 and 9; Bad, Bax, Bcl\u20102, Bim, c\u2010myc, Bag\u20101, as well as p53) was assessed by reverse transcription\u2010polymerase chain reaction (RT\u2010PCR). Samples were obtained from impression cytology (IC) specimens taken from 50 healthy subjects. Group A comprised 27 subjects aged 19\u221232\u2003years and group B included 23 subjects aged 53\u221284\u2003years.<\/jats:bold> <\/jats:p><jats:p><jats:bold>Results:\u2002<\/jats:bold>  <jats:bold>Reverse transcription\u2010PCR revealed the detection of apoptosis\u2010related m\u2010RNAs as follows (group A compared to group B): Apaf\u20101 0%\/0%; Bcl\u20102 0%\/35%; Bim 0%\/9%; Bag\u20101 0%\/9%; p53 0%\/4%; casp\u20103 11%\/52%; casp\u20105 59%\/48%; casp\u20108 44%\/22%; casp\u20109 4%\/9%; Bax 81%\/52%; Bad 96%\/56%, and c\u2010myc 89%\/96%.<\/jats:bold> <\/jats:p><jats:p><jats:bold>Conclusion:\u2002<\/jats:bold>  <jats:bold>The data show an age\u2010related expression of apoptosis\u2010related genes such as casp\u20103, Bad, Bax and Bcl\u20102 in normal conjunctival cells. These results provide basic information which will help us understand the expression pattern of apoptotic genes during physiological ageing of the conjunctiva and the possible dysregulation of apoptotic genes during acute and chronic diseases such as dry eye disease, allergic conjunctivitis or cicatrizing conjunctivitis.<\/jats:bold> <\/jats:p>","DOI":"10.1111\/j.1600-0420.2005.00472.x","type":"journal-article","created":{"date-parts":[[2005,7,20]],"date-time":"2005-07-20T01:30:57Z","timestamp":1121823057000},"page":"471-476","source":"Crossref","is-referenced-by-count":23,"title":["Age\u2010related differential expression of apoptosis\u2010related genes in conjunctival epithelial cells"],"prefix":"10.1111","volume":"83","author":[{"given":"J\u00fcrgen","family":"Giebel","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Christian","family":"Woenckhaus","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michael","family":"Fabian","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Frank","family":"Tost","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,5,24]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.1136\/bjo.62.11.792"},{"key":"e_1_2_5_3_1","first-page":"829","article-title":"The eye and nutrition","volume":"103","author":"Amemiya T","year":"1999","journal-title":"Nippon Ganka Gakkai Zasshi"},{"key":"e_1_2_5_4_1","first-page":"1458","article-title":"Flow cytometry in impression cytology specimens. 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