{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,3]],"date-time":"2025-11-03T09:02:52Z","timestamp":1762160572064},"reference-count":16,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2006,10,10]],"date-time":"2006-10-10T00:00:00Z","timestamp":1160438400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Acta Ophthalmologica Scandinavica"],"published-print":{"date-parts":[[2007,3]]},"abstract":"<jats:title>Abstract.<\/jats:title><jats:p><jats:bold>Purpose:\u2002<\/jats:bold> To characterize inherent light scattering in the C57BL\/6 mouse lens.<\/jats:p><jats:p><jats:bold>Methods:\u2002<\/jats:bold> Lenses from 20 6\u2010week\u2010old female C57BL\/6 mice were extracted from freshly enucleated globes and microsurgically cleaned of remnants of the ciliary body. Lens light scattering was measured quantitatively with a light dissemination meter (LDM). Morphological properties of the mouse lenses were documented using grid\u2010 and dark\u2010field illumination photography. Analysis of variance was performed to establish variance for animals, variance between left and right eyes and variance for measurements.<\/jats:p><jats:p><jats:bold>Results:\u2002<\/jats:bold> Average inherent light scattering in the C57BL\/6 mouse lens is 0.16\u2003\u00b1\u20030.02\u2003tEDC (transformed equivalent diazepam concentration). The mean size of a mouse lens at 6\u2003weeks is 1.9\u2003mm in diameter. Two lenses featured pre\u2010existing cortical lens opacities. Variance for animals was assessed to be 7.9 10<jats:sup>\u2212\u20034<\/jats:sup>\u2003tEDC<jats:sup>2<\/jats:sup>, variance for measurements was 1.6 10<jats:sup>\u2212\u20034<\/jats:sup>\u2003tEDC<jats:sup>2<\/jats:sup>, and variance between left and right eyes was 8.8 10<jats:sup>\u2212\u20034<\/jats:sup> tEDC<jats:sup>2<\/jats:sup>. The tolerance limit for non\u2010pathological light scattering was determined to 0.26\u2003tEDC. No significant difference in light scattering between left and right mouse lenses was found. The minimum number of C57BL\/6 mice required for detection of a 10% experimentally induced change in light scattering intensity was estimated to be 50 for independent group experiments and 25 for paired design experiments.<\/jats:p><jats:p><jats:bold>Conclusions:\u2002<\/jats:bold> The C57BL\/6 mouse is a suitable animal in which to conduct experiments on light scattering or cataractogenesis with high precision at reasonable sample sizes. Before including C57BL\/6 mice into a study on cataractogenesis, pre\u2010existing lens opacities such as congenital cataract must be excluded.<\/jats:p>","DOI":"10.1111\/j.1600-0420.2006.00800.x","type":"journal-article","created":{"date-parts":[[2006,10,10]],"date-time":"2006-10-10T08:26:23Z","timestamp":1160468783000},"page":"178-182","source":"Crossref","is-referenced-by-count":5,"title":["Light scattering in the C57BL\/6 mouse lens"],"prefix":"10.1111","volume":"85","author":[{"given":"Linda M.","family":"Meyer","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiuqin","family":"Dong","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alfred","family":"Wegener","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Per","family":"S\u00f6derberg","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2006,10,10]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00347-003-0795-1"},{"key":"e_1_2_6_3_1","first-page":"249","article-title":"Cataract blindness \u2013 challenges for the 21st century","volume":"79","author":"Brian G","year":"2001","journal-title":"Bull World Health Organ"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1006\/exer.1999.0687"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1076\/opge.24.1.1.13888"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1007\/s003470050072"},{"key":"e_1_2_6_7_1","first-page":"6033","volume-title":"The Metabolic and Molecular Basis of Inherited Disease","author":"Hejtmancik JF","year":"2001"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.exer.2004.03.014"},{"key":"e_1_2_6_9_1","first-page":"1","article-title":"The conquest of cataract: a global challenge","volume":"104","author":"Kupfer C","year":"1985","journal-title":"Trans Ophthalmol Soc U K"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1093\/oxfordjournals.aje.a113625"},{"key":"e_1_2_6_11_1","first-page":"4","article-title":"Monograph series on ageing\u2010related diseases. 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