{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T12:34:31Z","timestamp":1790685271166,"version":"4.1.0"},"reference-count":31,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2006,11,28]],"date-time":"2006-11-28T00:00:00Z","timestamp":1164672000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Acta Ophthalmologica Scandinavica"],"published-print":{"date-parts":[[2007,6]]},"abstract":"<jats:title>Abstract.<\/jats:title><jats:p><jats:bold>Purpose:\u2002<\/jats:bold> The maximum post\u2010mortem time limit for obtaining donor corneas varies between eye banks. It is not known for how long a time the epithelial cells survive post\u2010mortem, nor is it known if donor corneas with extended post\u2010mortem time are able to regenerate the epithelium. Therefore, we wanted to examine the epithelium in donor corneas for regenerative ability during storage in an eye bank organ culture system.<\/jats:p><jats:p><jats:bold>Methods:\u2002<\/jats:bold> Twenty\u2010four paired donor corneas with post\u2010mortem time from 28 to 163\u2003hr were obtained. One cornea of a pair was fixed immediately to serve as a control, and the second was cultured in eye bank medium at 32\u00b0C for 3\u2003days. Examination of the specimens was performed with light and scanning electron microscopy. Immunohistochemical staining methods with antibodies against K 3, K 19, vimentin and p63 were used to further characterize the cells.<\/jats:p><jats:p><jats:bold>Results:\u2002<\/jats:bold> The control corneas showed decreasing amounts of epithelial cells with increasing post\u2010mortem time. All the cultured corneas demonstrated rapid regeneration of the epithelium. After 3\u2003days in organ culture, 10 of 12 donor corneas were covered with epithelium.<\/jats:p><jats:p><jats:bold>Conclusion:\u2002<\/jats:bold> Even up to 7\u2003days post\u2010mortem, viable cells reside in the corneal epithelium. The study demonstrates the hardiness and enormous regenerative potential of peripheral corneal cells. Donor corneas processed in an eye bank organ culture storage system will obtain an intact epithelial layer within a few days.<\/jats:p>","DOI":"10.1111\/j.1600-0420.2006.00840.x","type":"journal-article","created":{"date-parts":[[2006,11,28]],"date-time":"2006-11-28T13:34:23Z","timestamp":1164720863000},"page":"371-376","source":"Crossref","is-referenced-by-count":23,"title":["Regeneration of the epithelium in organ\u2010cultured donor corneas with extended post\u2010mortem time"],"prefix":"10.1111","volume":"85","author":[{"given":"Jon 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