{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,28]],"date-time":"2026-08-28T03:40:52Z","timestamp":1787888452850,"version":"build-2784847793"},"reference-count":36,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2007,3,27]],"date-time":"2007-03-27T00:00:00Z","timestamp":1174953600000},"content-version":"vor","delay-in-days":2490,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["APMIS"],"published-print":{"date-parts":[[2000,6]]},"abstract":"<jats:p>For the steroidal growth promoters trenbolone acetate (TBA) and melengestrol acetate (MGA) neither the complete spectrum of biological activities nor the potential endocrine disrupting activity of their excreted metabolites in the environment is fully understood. The potency of these substances in [<jats:sup>3<\/jats:sup>H]\u2010dihydrotestosterone ([<jats:sup>3<\/jats:sup>H]\u2010DHT) displacement from the recombinant human androgen receptor (rhAR) and from human sex\u2010hormone binding globulin (hSHBG) was evaluated. In addition, the potency for [<jats:sup>3<\/jats:sup>H]\u2010ORG2058 displacement from the bovine uterine progestin receptor (bPR) was tested. For comparison, different anabolics and synthetic hormones were also tested for their binding affinities. For 17\u03b2\u2010trenbolone (17\u03b2\u2010TbOH), the active compound after TBA administration, an affinity the rhAR similar to dihydrotestosterone (DHT) and a slightly higher affinity to the bPR than progesterone were demonstrated. The affinity of the two major metabolites, 17\u03b1\u2010trenbolone and trendione, was reduced to less than 5% of the 17\u03b2\u2010TbOH\u2010value. The affinity of these three compounds and of MGA to the hSHBG was much lower compared with DHT. MGA showed a 5.3\u2010fold higher affinity than progesterone to the bPR but only a weak affinity to the rhAR. The major MGA metabolites have an affinity to the bPR between 85% and 28% of the affinity of progesterone. In consequence, MGA and TBA metabolites may be hor\u2010monally active substances, which will be present in edible tissues and in manure. We conclude that detailed investigations on biodegradation, distribution and bio\u2010efficacy of these substances are necessary.<\/jats:p>","DOI":"10.1111\/j.1600-0463.2000.tb00007.x","type":"journal-article","created":{"date-parts":[[2007,3,27]],"date-time":"2007-03-27T07:38:25Z","timestamp":1174981105000},"page":"838-846","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":93,"title":["Characterisation of the affinity of different anabolics and synthetic hormones to the human androgen receptor, human sex hormone binding globulin and to the bovine progestin receptor"],"prefix":"10.1111","volume":"108","author":[{"given":"E. R. 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