{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,9,1]],"date-time":"2023-09-01T06:12:17Z","timestamp":1693548737851},"reference-count":26,"publisher":"Wiley","issue":"7-8","license":[{"start":{"date-parts":[[2005,7,28]],"date-time":"2005-07-28T00:00:00Z","timestamp":1122508800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["APMIS"],"published-print":{"date-parts":[[2005,8]]},"abstract":"<jats:p>In humans and cattle, multiple injections of murine monoclonal antibodies (m\u2010mAbs) induce anti\u2010mouse antibody responses. The objectives of the present study were to investigate whether a similar response could be seen when pigs were subjected to m\u2010mAb therapy, and to study the kinetics of such a response. In two separate animal experiments, long\u2010term treatment was performed with m\u2010mAbs at low\u2010dose levels and therapeutic levels, respectively. Two specific m\u2010mAbs that recognized cognate antigen in the pigs (CD4 and CD8 surface antigens on T\u2010lymphocytes) and two irrelevant control m\u2010mAbs having no cognate antigen in the pigs were used. Enzyme\u2010linked immunosorbent assays (ELISA) were used to quantitate the circulating m\u2010mAbs, as well as the induced pig anti\u2010mouse antibodies (PAMA), in serum samples from m\u2010mAb\u2010treated pigs. As expected, we generally saw vigorous PAMA responses within 10 days after the start of m\u2010mAb treatment with the specific m\u2010mAbs. However, the different mAbs showed striking differences in the kinetics and levels of PAMA responses, differences that might be ascribed to the m\u2010mAb formulation and epitope specificity. In conclusion, treatment of pigs with m\u2010mAbs against T\u2010cell surface antigens induced rapid PAMA responses. This may influence and possibly decrease the effect of the m\u2010mAb treatment by narrowing the time period where m\u2010mAbs can efficiently be used for cell depletion.<\/jats:p>","DOI":"10.1111\/j.1600-0463.2005.apm_62.x","type":"journal-article","created":{"date-parts":[[2005,7,28]],"date-time":"2005-07-28T15:35:34Z","timestamp":1122564934000},"page":"489-496","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Porcine humoral immune responses to multiple injections of murine monoclonal antibodies"],"prefix":"10.1111","volume":"113","author":[{"given":"LOUISE","family":"LOHSE","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"JENS","family":"NIELSEN","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S\u00d8REN","family":"KAMSTRUP","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"MARTIN B.","family":"OLEKSIEWICZ","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"LIS","family":"ERIKSEN","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,7,28]]},"reference":[{"key":"e_1_2_6_2_2","doi-asserted-by":"publisher","DOI":"10.1097\/00007890-199007000-00014"},{"key":"e_1_2_6_3_2","doi-asserted-by":"publisher","DOI":"10.1097\/00002371-199408000-00004"},{"key":"e_1_2_6_4_2","doi-asserted-by":"publisher","DOI":"10.1097\/00007890-199909270-00019"},{"key":"e_1_2_6_5_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.135.2.1530"},{"key":"e_1_2_6_6_2","doi-asserted-by":"publisher","DOI":"10.1002\/eji.1830190428"},{"key":"e_1_2_6_7_2","doi-asserted-by":"publisher","DOI":"10.1089\/hyb.1989.8.231"},{"key":"e_1_2_6_8_2","first-page":"406","article-title":"Prolonged survival of nonhuman primate renal allograft recipients treated only with anti\u2010CD4 monoclonal antibody","volume":"108","author":"Cosimi AB","year":"1990","journal-title":"Surgery"},{"key":"e_1_2_6_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0165-2427(99)00098-7"},{"key":"e_1_2_6_10_2","doi-asserted-by":"crossref","first-page":"368","DOI":"10.4049\/jimmunol.133.1.368","article-title":"Preparation and characterization of monoclonal antibodies reactive with porcine PBL","volume":"133","author":"Pescovitz MD","year":"1984","journal-title":"J Immunol"},{"key":"e_1_2_6_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-1135(96)01334-X"},{"key":"e_1_2_6_12_2","doi-asserted-by":"publisher","DOI":"10.1089\/08828240260066297"},{"key":"e_1_2_6_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0165-2427(03)00031-X"},{"key":"e_1_2_6_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0165-2427(03)00068-0"},{"key":"e_1_2_6_15_2","doi-asserted-by":"publisher","DOI":"10.1038\/256495a0"},{"key":"e_1_2_6_16_2","first-page":"64","volume-title":"Preparation of a porcine parvovirus vaccin","author":"Lei JC","year":"1980"},{"key":"e_1_2_6_17_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-1135(02)00174-8"},{"key":"e_1_2_6_18_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-1135(96)01272-2"},{"key":"e_1_2_6_19_2","doi-asserted-by":"publisher","DOI":"10.1089\/hyb.1997.16.557"},{"key":"e_1_2_6_20_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0003-4975(97)00796-0"},{"key":"e_1_2_6_21_2","doi-asserted-by":"publisher","DOI":"10.1016\/0378-1135(91)90095-W"},{"key":"e_1_2_6_22_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-1135(01)00450-3"},{"key":"e_1_2_6_23_2","doi-asserted-by":"publisher","DOI":"10.1038\/312548a0"},{"key":"e_1_2_6_24_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.163.6.1539"},{"key":"e_1_2_6_25_2","doi-asserted-by":"publisher","DOI":"10.2165\/00063030-200216010-00005"},{"key":"e_1_2_6_26_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-065X.1992.tb01519.x"},{"key":"e_1_2_6_27_2","doi-asserted-by":"publisher","DOI":"10.1038\/321522a0"}],"container-title":["APMIS"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fj.1600-0463.2005.apm_62.x","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/j.1600-0463.2005.apm_62.x","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,31]],"date-time":"2023-08-31T09:06:57Z","timestamp":1693472817000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/j.1600-0463.2005.apm_62.x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2005,7,28]]},"references-count":26,"journal-issue":{"issue":"7-8","published-print":{"date-parts":[[2005,8]]}},"alternative-id":["10.1111\/j.1600-0463.2005.apm_62.x"],"URL":"https:\/\/doi.org\/10.1111\/j.1600-0463.2005.apm_62.x","archive":["Portico"],"relation":{},"ISSN":["0903-4641","1600-0463"],"issn-type":[{"value":"0903-4641","type":"print"},{"value":"1600-0463","type":"electronic"}],"subject":[],"published":{"date-parts":[[2005,7,28]]},"assertion":[{"value":"2005-07-28","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}