{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,10,14]],"date-time":"2023-10-14T16:32:46Z","timestamp":1697301166903},"reference-count":27,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2006,5,24]],"date-time":"2006-05-24T00:00:00Z","timestamp":1148428800000},"content-version":"vor","delay-in-days":23,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["APMIS"],"published-print":{"date-parts":[[2006,5]]},"abstract":"<jats:p>For over 35 years hemolytic disease of the fetus and newborn (HDFN) due to RhD has been effectively prevented by anti\u2010RhD antibodies obtained from alloimmunized women or deliberately immunized men. However, due to the reduced number of immunized women and for ethical reasons it is foreseen that other sources of anti\u2010RhD will be needed. One such source is recombinant human antibodies. Here we describe the construction of plasmids encoding two subclasses (IgG1 and IgG3) of an anti\u2010RhD antibody, their transient expression in COS cells, and subsequent functional characterization of the antibodies with regard to specificity and ability to mediate a respiratory burst. The recombinant anti\u2010RhD antibodies were specific for the RhD antigen and were able to mediate a respiratory burst. Thus these antibodies might be of use as future rhesus prophylaxis.<\/jats:p>","DOI":"10.1111\/j.1600-0463.2006.apm_381.x","type":"journal-article","created":{"date-parts":[[2006,5,24]],"date-time":"2006-05-24T13:02:44Z","timestamp":1148475764000},"page":"345-351","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["<i>In vitro<\/i> functional test of two subclasses of an anti\u2010RhD antibody produced by transient expression in COS cells"],"prefix":"10.1111","volume":"114","author":[{"given":"LEIF KOFOED","family":"NIELSEN","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"LARS","family":"NORDERHAUG","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"INGER","family":"SANDLIE","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"MORTEN HANEFELD","family":"DZIEGIEL","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2006,5,24]]},"reference":[{"key":"e_1_2_7_2_2","doi-asserted-by":"crossref","first-page":"1701","DOI":"10.1182\/blood.V86.5.1701.bloodjournal8651701","article-title":"Human Rh D monoclonal antibodies (BRAD\u20103 and BRAD\u20105) cause accelerated clearance of Rh D+ red blood cells and suppression of Rh D immunization in Rh D\u2010volunteers","volume":"86","author":"Kumpel BM","year":"1995","journal-title":"Blood"},{"issue":"2","key":"e_1_2_7_3_2","doi-asserted-by":"crossref","first-page":"561","DOI":"10.1042\/bj2770561","article-title":"Expression of a human monoclonal anti\u2010(rhesus D) Fab fragment in Escherichia coli with the use of bacteriophage lambda vectors","volume":"277","author":"Williamson RA","year":"1991","journal-title":"Biochem J"},{"key":"e_1_2_7_4_2","doi-asserted-by":"crossref","first-page":"2334","DOI":"10.1182\/blood.V83.8.2334.2334","article-title":"Expression and characterization of recombinant anti\u2010Rh(D) antibodies on filamentous phage: a model system for isolating human red blood cell antibodies by repertoire cloning","volume":"83","author":"Siegel DL","year":"1994","journal-title":"Blood"},{"key":"e_1_2_7_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/0022-1759(95)00013-Z"},{"key":"e_1_2_7_6_2","doi-asserted-by":"publisher","DOI":"10.1038\/nbt1093-1145"},{"key":"e_1_2_7_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-1759(97)00087-2"},{"key":"e_1_2_7_8_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1423-0410.1998.7540278.x"},{"key":"e_1_2_7_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/0167-5699(90)90126-T"},{"key":"e_1_2_7_10_2","first-page":"481","article-title":"Functional interactions of aglycosylated monoclonal anti\u2010D with Fc gamma RI+ and Fc gamma RIII+ cells","volume":"72","author":"Leader KA","year":"1991","journal-title":"Immunology"},{"key":"e_1_2_7_11_2","doi-asserted-by":"publisher","DOI":"10.1023\/A:1015226418688"},{"key":"e_1_2_7_12_2","doi-asserted-by":"crossref","first-page":"17","DOI":"10.3233\/HAB-1997-8103","article-title":"Human recombinant anti\u2010Rh(D) monoclonal antibodies: improvement of biological properties by in vitro class\u2010switch","volume":"8","author":"Klein P","year":"1997","journal-title":"Hum Antibodies"},{"key":"e_1_2_7_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1380-2933(98)00005-0"},{"key":"e_1_2_7_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0966-3274(02)00066-7"},{"key":"e_1_2_7_15_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2257.1989.tb00173.x"},{"key":"e_1_2_7_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-1759(97)00034-3"},{"key":"e_1_2_7_17_2","doi-asserted-by":"crossref","first-page":"306","DOI":"10.4269\/ajtmh.1991.44.306","article-title":"Recombinant Plasmodium falciparum glutamate rich protein; purification and use in enzyme\u2010linked immunosorbent assay","volume":"44","author":"Dziegiel M","year":"1991","journal-title":"Am J Trop Med Hyg"},{"key":"e_1_2_7_18_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2257.1988.tb01184.x"},{"key":"e_1_2_7_19_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.coph.2003.05.002"},{"key":"e_1_2_7_20_2","doi-asserted-by":"publisher","DOI":"10.1002\/eji.1830191216"},{"key":"e_1_2_7_21_2","first-page":"77","article-title":"The effector functions of immunoglobulins: implications for therapy","volume":"2","author":"Ward ES","year":"1995","journal-title":"Ther Immunol"},{"key":"e_1_2_7_22_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2141.2000.02322.x"},{"key":"e_1_2_7_23_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1246-7820(96)80068-1"},{"key":"e_1_2_7_24_2","doi-asserted-by":"publisher","DOI":"10.1016\/0161-5890(90)90137-O"},{"key":"e_1_2_7_25_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1744-313X.1990.tb00883.x"},{"key":"e_1_2_7_26_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0950-3536(06)80036-3"},{"key":"e_1_2_7_27_2","article-title":"Selection of a human anti\u2010RhD monoclonal antibody for therapeutic use: impact of IgG glycosylation on activating and inhibitory FcgammaR functions","volume":"2","author":"Siberil S","year":"2005","journal-title":"Clin Immunol"},{"key":"e_1_2_7_28_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2141.2005.05796.x"}],"container-title":["APMIS"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fj.1600-0463.2006.apm_381.x","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/j.1600-0463.2006.apm_381.x","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,11]],"date-time":"2023-10-11T03:59:39Z","timestamp":1696996779000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/j.1600-0463.2006.apm_381.x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2006,5]]},"references-count":27,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2006,5]]}},"alternative-id":["10.1111\/j.1600-0463.2006.apm_381.x"],"URL":"https:\/\/doi.org\/10.1111\/j.1600-0463.2006.apm_381.x","archive":["Portico"],"relation":{},"ISSN":["0903-4641","1600-0463"],"issn-type":[{"value":"0903-4641","type":"print"},{"value":"1600-0463","type":"electronic"}],"subject":[],"published":{"date-parts":[[2006,5]]},"assertion":[{"value":"2006-05-24","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}