{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,10,12]],"date-time":"2023-10-12T05:31:02Z","timestamp":1697088662372},"reference-count":20,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2006,5,4]],"date-time":"2006-05-04T00:00:00Z","timestamp":1146700800000},"content-version":"vor","delay-in-days":33,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["APMIS"],"published-print":{"date-parts":[[2006,4]]},"abstract":"<jats:p>Serological diagnosis of <jats:italic>Chamydophila pneumoniae<\/jats:italic> is usually undertaken by complement fixation test (CFT) or by microimmunofluorescence (MIF). A number of commercial methods for detecting <jats:italic>C. pneumoniae<\/jats:italic>\u2010specific IgG have been developed. The aim of this study was to compare the performance characteristics of six methods for the diagnosis of pneumonia due to <jats:italic>C. pneumoniae<\/jats:italic>, including CFT (in house), MIF (Vircell, Spain), and four ELISAs (Medac, Germany; Savyon, Israel; Serion, Germany; and DRG, Germany). ELISA\u2010Medac, ELISA\u2010Savyon, ELISA\u2010DRG and MIF use <jats:italic>C. pneumoniae<\/jats:italic> antigens while ELISA\u2010Serion and CFT use <jats:italic>Chlamydophila<\/jats:italic> genus\u2010specific antigen. Acute and convalescent samples from 85 pneumonia patients were studied. Using CFT, cases were initially classified as due to <jats:italic>Chlamydophila<\/jats:italic> (43 cases); to other agents (23 cases) (influenza A and B, <jats:italic>Mycoplasma pneumoniae<\/jats:italic>, respiratory syncytial virus, adenovirus and <jats:italic>Legionella pneumophila<\/jats:italic>); or as negative (19 cases). Cases were considered positive if they showed seroconversion, a significant rise in titer or high titer; and were finally classified as positive if they gave a positive result in at least three assays. Sensitivity values ranged from 87% to 97.8%; and specificity from 84.6% to 97.4%. In conclusion, the assays compared appear to be useful tools for the diagnosis of pneumonia due to <jats:italic>Chlamydophila<\/jats:italic>.<\/jats:p>","DOI":"10.1111\/j.1600-0463.2006.apm_385.x","type":"journal-article","created":{"date-parts":[[2006,5,4]],"date-time":"2006-05-04T09:57:59Z","timestamp":1146736679000},"page":"279-284","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Detection of <i>Chlamydophila pneumoniae<\/i> IgG in paired serum samples: comparison of serological techniques in pneumonia cases"],"prefix":"10.1111","volume":"114","author":[{"given":"FERNANDO","family":"DE ORY","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"MAR\u00cdA EULALIA","family":"GUISASOLA","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"JOS\u00c9 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