{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,10,11]],"date-time":"2023-10-11T07:46:26Z","timestamp":1697010386448},"reference-count":31,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2008,12,30]],"date-time":"2008-12-30T00:00:00Z","timestamp":1230595200000},"content-version":"vor","delay-in-days":59,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["APMIS"],"published-print":{"date-parts":[[2008,11]]},"abstract":"<jats:p>Staphylococci are a common cause of catheter\u2010related bloodstream infection (CR\u2010BSI), and epidemiological typing is an important tool for effective infection control. This study evaluated by PFGE and rep\u2010PCR whether <jats:italic>Staphylococcus aureus<\/jats:italic> strains isolated from skin and catheter tips were related to specimens isolated from blood. A prospective observational study, carried out in a clinical surgical ward at a Brazilian hospital between September 2000 and November 2002, investigated non\u2010tunneled central venous catheters from 179 patients. <jats:italic>S. aureus <\/jats:italic>isolates were mainly obtained from blood (41.4%), while coagulase\u2010negative staphylococci strains were more often isolated from the skin at the catheter insertion site (49.7%) and from the catheter tip (57.5%). Among the 21 strains isolated from 9 patients at 2 or 3 sites simultaneously, 9 were methicillin\u2010resistant <jats:italic>S. aureus<\/jats:italic> (MRSA) and 12 were methicillin\u2010susceptible <jats:italic>S. aureus<\/jats:italic> (MSSA). Seven patients harbored the same <jats:italic>S. aureus<\/jats:italic> strain isolated from the skin, blood and\/or catheter tip cultures. MRSA isolates belonged to one PFGE pattern (type A\u2010 subtypes A<jats:sub>1<\/jats:sub>, A<jats:sub>2<\/jats:sub> and A<jats:sub>3<\/jats:sub>), and to two rep\u2010PCR patterns (a and b). MSSA isolates were distinguished in five PFGE (B to F) and in three rep\u2010PCR (c, d and e) patterns. Both PFGE and rep\u2010PCR methods indicated that the skin at the catheter insertion site was the origin of CR\u2010BSI caused by <jats:italic>S. aureus<\/jats:italic>.<\/jats:p>","DOI":"10.1111\/j.1600-0463.2008.01053.x","type":"journal-article","created":{"date-parts":[[2008,12,31]],"date-time":"2008-12-31T20:58:11Z","timestamp":1230757091000},"page":"953-960","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["<i>Staphylococcus aureus<\/i> as source of catheter\u2010related bloodstream infection evaluated by PFGE and rep\u2010PCR typing in a Brazilian hospital"],"prefix":"10.1111","volume":"116","author":[{"given":"GERALDO","family":"SADOYAMA","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"K\u00c1TIA REGINA NETTO DOS","family":"SANTOS","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"ANIKE PEREIRA","family":"BRILHANTE","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"PAULO PINTO GONTIJO","family":"FILHO","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2008,12,30]]},"reference":[{"key":"e_1_2_6_2_2","doi-asserted-by":"publisher","DOI":"10.1017\/S0195941700080577"},{"key":"e_1_2_6_3_2","volume-title":"Catheter\u2010related Infections","author":"Sheretz RJ","year":"1997"},{"key":"e_1_2_6_4_2","doi-asserted-by":"publisher","DOI":"10.1053\/jhin.2002.1349"},{"key":"e_1_2_6_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(04)01003-5"},{"key":"e_1_2_6_6_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1523-1755.1998.00134.x"},{"key":"e_1_2_6_7_2","doi-asserted-by":"publisher","DOI":"10.1093\/ndt\/13.11.2847"},{"key":"e_1_2_6_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jinf.2005.06.005"},{"key":"e_1_2_6_9_2","doi-asserted-by":"publisher","DOI":"10.1136\/jcp.55.11.824"},{"key":"e_1_2_6_10_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.42.4.1402-1408.2004"},{"key":"e_1_2_6_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0732-8893(02)00469-8"},{"key":"e_1_2_6_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajic.2003.09.002"},{"key":"e_1_2_6_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajic.2004.05.003"},{"key":"e_1_2_6_14_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-0691.2006.01491.x"},{"key":"e_1_2_6_15_2","doi-asserted-by":"publisher","DOI":"10.1078\/1438-4221-00121"},{"key":"e_1_2_6_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/0140-6736(91)90479-9"},{"key":"e_1_2_6_17_2","doi-asserted-by":"crossref","first-page":"873","DOI":"10.1001\/archinte.1987.00370050069012","article-title":"Diagnosis of central venous catheter\u2010related sepsis: critical level of quantitative tip cultures","volume":"147","author":"Brun\u2010Buisson C","year":"1987","journal-title":"Arch Intern Med"},{"key":"e_1_2_6_18_2","volume-title":"Manual of Clinical Microbiology","author":"Bannerman TL","year":"2003"},{"key":"e_1_2_6_19_2","volume-title":"Methods for dilution antimicrobial susceptibility tests for bacteria that grow aerobically. 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