{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,29]],"date-time":"2025-12-29T18:48:58Z","timestamp":1767034138958},"reference-count":16,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2009,2,20]],"date-time":"2009-02-20T00:00:00Z","timestamp":1235088000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["APMIS"],"published-print":{"date-parts":[[2009,4]]},"abstract":"<jats:p>Lysosome\u2010associated membrane proteins 1 and 2 (LAMP\u20101 and LAMP\u20102) are implicated in a variety of normal and pathological processes. LAMP\u20102 is proposed to participate in chaperone\u2010mediated autophagy. Autophagy regulates T\u2010lymphocyte homeostasis by promoting both survival and proliferation. The biological importance of this process in the thymic gland and especially the involvement of LAMPs are far from being elucidated. The aim of the study was to examine the parallel expression of LAMPs and ubiquitin, a key molecule in autophagy, in normal human thymic glands and thymomas. The immunohistochemical expression of both markers was compared with that of cyclin D1 \u2013 an important regulator of cell cycle progression. Novel evidence for differential expression of LAMPs and ubiquitin is presented. Most Hassal's corpuscules in thymoma were negative for LAMPs, but positive in normal thymus. Both lymphocytes and epithelial cells in pathological thymus showed higher intensity for LAMP\u20102 compared with LAMP\u20101. In thymoma, ubiquitin was more intensively positive in these cell types compared with the normal thymus, suggesting activated autophagy in the course of this pathological state. A deregulation in cyclin D1 expression in thymoma is also reported. The functional importance of these molecules in autoghagy accompanying normal and pathological processes in the thymic gland is reviewed.<\/jats:p>","DOI":"10.1111\/j.1600-0463.2009.02437.x","type":"journal-article","created":{"date-parts":[[2009,2,20]],"date-time":"2009-02-20T09:50:22Z","timestamp":1235123422000},"page":"248-252","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Differential expression of LAMPs and ubiquitin in human thymus"],"prefix":"10.1111","volume":"117","author":[{"given":"VICTORIA S.","family":"SARAFIAN","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"TSVETANA T.","family":"MARINOVA","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"MAYA V.","family":"GULUBOVA","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2009,3,26]]},"reference":[{"key":"e_1_2_6_2_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.273.5274.501"},{"key":"e_1_2_6_3_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.emboj.7601511"},{"key":"e_1_2_6_4_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0463.2007.apm_576.x"},{"key":"e_1_2_6_5_2","doi-asserted-by":"publisher","DOI":"10.1556\/ABiol.57.2006.3.5"},{"key":"e_1_2_6_6_2","doi-asserted-by":"publisher","DOI":"10.4161\/auto.3908"},{"key":"e_1_2_6_7_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature07208"},{"key":"e_1_2_6_8_2","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.00481.2005"},{"key":"e_1_2_6_9_2","doi-asserted-by":"publisher","DOI":"10.4161\/cc.6.15.4511"},{"key":"e_1_2_6_10_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature06639"},{"key":"e_1_2_6_11_2","first-page":"259","article-title":"NGF overexpression and distribution in cortical thymic epithelial cells of mice with experimentally\u2010induced leukemia","volume":"20","author":"Marinova TS","year":"2006","journal-title":"In Vivo"},{"key":"e_1_2_6_12_2","first-page":"12","article-title":"Thymus and myasthenia gravis","volume":"183","author":"Melms A","year":"2006","journal-title":"antigen processing in the human thymus and the consequences for the generation of autoreactive T cells"},{"key":"e_1_2_6_13_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature03886"},{"key":"e_1_2_6_14_2","doi-asserted-by":"crossref","first-page":"4441","DOI":"10.1242\/jcs.113.24.4441","article-title":"Unique properties of lamp2a compared to other lamp2 isoforms","volume":"24","author":"Cuervo AM","year":"2000","journal-title":"J Cell Sci"},{"key":"e_1_2_6_15_2","first-page":"24","article-title":"The regulation of cyclin D1 degradation","volume":"6","author":"Alao JP.","year":"2007","journal-title":"roles in cancer development and the potential for therapeutic invention"},{"key":"e_1_2_6_16_2","first-page":"1005","article-title":"Immunohistochemical expression of p53, p21\/waf1, rb, p16, cyclin D1, p27, Ki67, cyclin A, cyclin B1, bcl2, bax and bak proteins and apoptotic index in normal thymus","volume":"6","author":"Kanavaros P","year":"2001","journal-title":"Histol Histopathol"},{"key":"e_1_2_6_17_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2559.2003.01663.x"}],"container-title":["APMIS"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fj.1600-0463.2009.02437.x","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/j.1600-0463.2009.02437.x","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,11]],"date-time":"2023-10-11T00:22:17Z","timestamp":1696983737000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/j.1600-0463.2009.02437.x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2009,3,26]]},"references-count":16,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2009,4]]}},"alternative-id":["10.1111\/j.1600-0463.2009.02437.x"],"URL":"https:\/\/doi.org\/10.1111\/j.1600-0463.2009.02437.x","archive":["Portico"],"relation":{},"ISSN":["0903-4641","1600-0463"],"issn-type":[{"value":"0903-4641","type":"print"},{"value":"1600-0463","type":"electronic"}],"subject":[],"published":{"date-parts":[[2009,3,26]]},"assertion":[{"value":"2009-03-26","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}