{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,8,9]],"date-time":"2024-08-09T17:54:55Z","timestamp":1723226095096},"reference-count":18,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2009,11,17]],"date-time":"2009-11-17T00:00:00Z","timestamp":1258416000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["APMIS"],"published-print":{"date-parts":[[2009,12]]},"abstract":"<jats:p>Johansson E, Fredlund H, Unemo M. Prevalence, phenotypic and genetic characteristics of prolyliminopeptidase\u2010negative <jats:italic>Neisseria gonorrhoeae<\/jats:italic> isolates in Sweden during 2000\u20132007. APMIS 2009; 117: 900\u20134.<\/jats:p><jats:p>In <jats:italic>Neisseria gonorrhoeae<\/jats:italic> culture diagnostics, species confirmation is commonly performed using commercial biochemical tests relying on prolyliminopeptidase (PIP) activity. It was previously shown that one PIP\u2010negative strain was mainly globally transmitted during 2000\u20132004. The aims were to investigate the prevalence and phenotypic and genetic characteristics of PIP\u2010negative <jats:italic>N.\u00a0gonorrhoeae<\/jats:italic> isolates in Sweden during 2000\u20132007. Gonococcal isolates (n\u2003=\u20031230) cultured in Sweden during 2000\u20132007 were characterized using PIP screening, antibiogram, serovar determination, <jats:italic>pip<\/jats:italic> and <jats:italic>porB<\/jats:italic> gene sequencing, and <jats:italic>N.\u00a0gonorrhoeae<\/jats:italic> multiantigen sequence typing (NG\u2010MAST). Fifteen (1.2%) of the isolates were PIP\u2010negative. Of those, 13 (87%) were indistinguishable to the previously described globally transmitted strain, i.e. displayed serovar IB\u20104 (Bpyvut), similar antibiograms, ST210 (n\u2003=\u200310)\/ST292 (n\u2003=\u20033) and contained an identical single nucleotide <jats:italic>pip<\/jats:italic> gene deletion. Wherever high reliance is placed on PIP activity for <jats:italic>N.\u00a0gonorrhoeae<\/jats:italic> species confirmation, changes in the diagnostic strategies may need to be considered and\/or monitoring of the PIP activity is crucial.<\/jats:p>","DOI":"10.1111\/j.1600-0463.2009.02552.x","type":"journal-article","created":{"date-parts":[[2009,11,17]],"date-time":"2009-11-17T22:02:58Z","timestamp":1258495378000},"page":"900-904","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Prevalence, phenotypic and genetic characteristics of prolyliminopeptidase\u2010negative <i>Neisseria gonorrhoeae<\/i> isolates in Sweden during 2000\u20132007"],"prefix":"10.1111","volume":"117","author":[{"given":"EMMA","family":"JOHANSSON","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"HANS","family":"FREDLUND","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"MAGNUS","family":"UNEMO","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2009,11,17]]},"reference":[{"key":"e_1_2_6_2_2","doi-asserted-by":"publisher","DOI":"10.2353\/jmoldx.2006.050045"},{"key":"e_1_2_6_3_2","doi-asserted-by":"crossref","first-page":"606","DOI":"10.7326\/0003-4819-148-8-200804150-00005","article-title":"Emerging antimicrobial resistance in Neisseria gonorrhoeae: urgent need to strengthen prevention strategies","volume":"148","author":"Workowski KA","year":"2008","journal-title":"Ann Intern Med"},{"key":"e_1_2_6_4_2","doi-asserted-by":"crossref","first-page":"268","DOI":"10.1503\/cmaj.081721","article-title":"Multidrug\u2010resistant Neisseria gonorrhoeae","volume":"180","author":"Tapsall J","year":"2009","journal-title":"CMAJ"},{"key":"e_1_2_6_5_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkp098"},{"key":"e_1_2_6_6_2","doi-asserted-by":"publisher","DOI":"10.1586\/eri.09.63"},{"key":"e_1_2_6_7_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.1993.tb01249.x"},{"key":"e_1_2_6_8_2","doi-asserted-by":"crossref","first-page":"4189","DOI":"10.1128\/JCM.43.8.4189-4190.2005","article-title":"Characterization of prolyl iminopeptidase\u2010deficient Neisseria gonorrhoeae","volume":"43","author":"Blackmore T","year":"2005","journal-title":"J Clin Microbiol"},{"key":"e_1_2_6_9_2","unstructured":"PalmerHM WuS GoughKR HemmingMN HassanI TurnerA.An increase in prolyliminopeptidase\u2010negative isolation of Neisseria gonorrhoeae in the United Kingdom is associated with the spread of one clonal strain of the organism. 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