{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,3]],"date-time":"2026-06-03T07:39:25Z","timestamp":1780472365091,"version":"3.54.1"},"reference-count":31,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2009,12,14]],"date-time":"2009-12-14T00:00:00Z","timestamp":1260748800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["APMIS"],"published-print":{"date-parts":[[2010,1]]},"abstract":"<jats:p>Badawi H, Ahmed H, Aboul Fadl L, Helmi A, Fam N, Diab M, Ismail A, Badawi A, Saber M. Herpes simplex virus type\u20102 in Egyptian patients with bladder cancer or cystitis. APMIS 2010; 118: 37\u201344.<\/jats:p><jats:p>The present study was designed to investigate the prevalence of herpes simplex virus type\u20102 (HSV\u20102) in Egyptian patients with bladder cancer or cystitis and to evaluate the performance of different diagnostic HSV\u20102 assays. The study included 50 patients: 27 with bladder cancer (group I), 23 with cystitis (group II) and 20 subjects as controls (group III). HSV\u20102 DNA was detected using polymerase chain reaction (PCR) on bladder tissue and buffy coat cells (BCC). Electron microscopic studies (EMS) on BCC and ELISAs for IgM, IgG and specific glycoprotein G\u20102 (gG\u20102) IgG were performed. HSV\u20102 DNA was detected by PCR on bladder tissue biopsies in 29.6% and 21.7% of group I and II respectively and it was also detected by PCR on BCC in 22.2% and 21.7% of group I and II respectively. EMS revealed HSV like particles in 16.6% of cases. IgG, specific gG\u20102 IgG and IgM were detected in 30%, 16% and 6% of cases respectively. The different assays were evaluated in relation to PCR on bladder tissue biopsies. The gG\u20102\u2010based ELISA and EMS on BCC were found to be highly specific (97.3% and 100% respectively), with similar low sensitivity of \u224854%. PCR on BCC was the most sensitive assay. The association of HSV\u20102 with bladder cancer is suggested especially in schistosomal patients.<\/jats:p>","DOI":"10.1111\/j.1600-0463.2009.02556.x","type":"journal-article","created":{"date-parts":[[2009,12,14]],"date-time":"2009-12-14T20:29:42Z","timestamp":1260822582000},"page":"37-44","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Herpes simplex virus type\u20102 in Egyptian patients with bladder cancer or cystitis"],"prefix":"10.1111","volume":"118","author":[{"given":"HALA","family":"BADAWI","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"HANEM","family":"AHMED","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"LAILA","family":"ABOUL FADL","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"AMIRA","family":"HELMI","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"NEVINE","family":"FAM","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"MANAL","family":"DIAB","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"AHMED","family":"ISMAIL","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"AFKAR","family":"BADAWI","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"MOHAMED","family":"SABER","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2009,12,14]]},"reference":[{"key":"e_1_2_7_2_2","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2407-8-250"},{"key":"e_1_2_7_3_2","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1096-9071(199808)55:4<262::AID-JMV2>3.0.CO;2-Z"},{"key":"e_1_2_7_4_2","doi-asserted-by":"publisher","DOI":"10.3923\/pjbs.2007.955.958"},{"key":"e_1_2_7_5_2","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1096-9071(199809)56:1<79::AID-JMV13>3.0.CO;2-R"},{"key":"e_1_2_7_6_2","doi-asserted-by":"crossref","first-page":"825","DOI":"10.1128\/jvi.54.3.825-832.1985","article-title":"Synthesis and processing of glycoprotein gG of herpes simplex virus type 2","volume":"54","author":"Balachandran N","year":"1985","journal-title":"J Virol"},{"key":"e_1_2_7_7_2","first-page":"193","volume-title":"Urol Res","author":"Sobin LH","year":"1978"},{"key":"e_1_2_7_8_2","first-page":"241","volume-title":"American Joint Committee on Cancer: AJCC Cancer Staging Manual","author":"Flemming I","year":"1997"},{"key":"e_1_2_7_9_2","first-page":"77","article-title":"Isolation of mononuclear cells and granulocytes from human blood","volume":"97","author":"Boyum A","year":"1968","journal-title":"Scand J Clin Lab Invest"},{"key":"e_1_2_7_10_2","unstructured":"AusubelMF BrentR KingstonRE MooreD SeidmanGJ SmithAJ et\u00a0al.Current Protocols in Molecular Biology. 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