{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,11,1]],"date-time":"2023-11-01T08:11:08Z","timestamp":1698826268059},"reference-count":47,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2010,2,1]],"date-time":"2010-02-01T00:00:00Z","timestamp":1264982400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["APMIS"],"published-print":{"date-parts":[[2010,3]]},"abstract":"<jats:p>Hasegawa T, Okamoto A, Kamimura T, Tatsuno I, Hashikawa S\u2010N, Yabutani M, Matsumoto M, Yamada K, Isaka M, Minami M, Ohta M. Detection of invasive protein profile of <jats:italic>Streptococcus pyogenes<\/jats:italic> M1 isolates from pharyngitis patients. APMIS 2010; 118: 167\u201378.<\/jats:p><jats:p>Streptococcal toxic shock syndrome (STSS) is a re\u2010emerging infectious disease in Japan and many other developed countries. Epidemiological studies have revealed that the M1 serotype of <jats:italic>Streptococcus pyogenes<\/jats:italic> is the most dominant causative isolate of STSS. Recent characterization of M1 isolates revealed that the mutation of <jats:italic>covS<\/jats:italic>, one of the two\u2010component regulatory systems, plays an important role in STSS by altering protein expression. We analyzed the M1 <jats:italic>S.\u00a0pyogenes<\/jats:italic> clinical isolates before or after 1990 in Japan, using two\u2010dimensional gel electrophoresis (2\u2010DE) and pulsed\u2010field gel electrophoresis (PFGE). PFGE profiles were different between the isolates before and after 1990. Markedly different profiles among isolates after 1990 from STSS and pharyngitis patients were detected. Sequence analysis of two\u2010component regulatory systems showed that <jats:italic>covS<\/jats:italic> mutations were detected not only in STSS but also in three pharyngitis isolates, in which proteins from the culture supernatant displayed the invasive type. The mutated CovS detected in the pharyngitis isolates had impaired function on the production of streptococcal pyrogenic exotoxin B (SpeB) analyzed by 2\u2010DE. These results suggest that several <jats:italic>covS<\/jats:italic> mutations that lead to the malfunction of the CovS protein occurred even in pharyngeal infection.<\/jats:p>","DOI":"10.1111\/j.1600-0463.2009.02574.x","type":"journal-article","created":{"date-parts":[[2010,2,1]],"date-time":"2010-02-01T11:11:00Z","timestamp":1265022660000},"page":"167-178","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":23,"title":["Detection of invasive protein profile of <i>Streptococcus pyogenes<\/i> M1 isolates from pharyngitis 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