{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,29]],"date-time":"2026-03-29T14:12:45Z","timestamp":1774793565131,"version":"3.50.1"},"reference-count":25,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2010,3,20]],"date-time":"2010-03-20T00:00:00Z","timestamp":1269043200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["APMIS"],"published-print":{"date-parts":[[2010,4]]},"abstract":"<jats:p>Tapiainen T, Kujala T, Kaijalainen T, Ik\u00e4heimo I, Saukkoriipi A, Renko M, Salo J, Leinonen M, Uhari M. Biofilm formation by <jats:italic>Streptococcus pneumoniae<\/jats:italic> isolates from paediatric patients. APMIS 2010; 118: 255\u201360.<\/jats:p><jats:p>The clinical significance of pneumococcal biofilm formation is largely unknown. To clarify this, we tested whether the ability of pneumococcal clinical isolates to form biofilm <jats:italic>in vitro<\/jats:italic> accounts for the diverse clinical outcomes. Clinical pneumococcal isolates were cultured from the nasopharynx (n\u2003=\u2003106), middle ear effusion (n\u2003=\u200343) and blood (n\u2003=\u200355) of 204 children altogether. Biofilm formation, assessed by measuring optical density (OD) values in microtitre plates after crystal violet staining, did not differ between the bacteria from different sources (p\u2003=\u20030.18), the mean OD values of the isolates being 0.119 [95% confidence interval (CI) 0.100\u20130.138] in the nasopharynx samples, 0.094 (95% CI 0.069\u20130.119) in the acute otitis media cases, 0.109 (95% CI 0.077\u20130.141) in the secretory otitis media cases, 0.122 (95% CI 0.084\u20130.160) in those with sepsis and 0.175 (95% CI 0.071\u20130.280) in those with other invasive infections. Serotypes 33 and 14 were the most efficient in forming biofilms, whereas serotypes 3 and 38 were poor biofilm producers. We conclude that the clinical presentation of pneumococcal disease did not differ in relation to biofilm formation <jats:italic>in vitro<\/jats:italic>, even though there was marked variation between the clinical isolates and serotypes.<\/jats:p>","DOI":"10.1111\/j.1600-0463.2010.02587.x","type":"journal-article","created":{"date-parts":[[2010,3,21]],"date-time":"2010-03-21T01:02:06Z","timestamp":1269133326000},"page":"255-260","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":20,"title":["Biofilm formation by <i>Streptococcus pneumoniae<\/i> isolates from paediatric patients"],"prefix":"10.1111","volume":"118","author":[{"given":"TERHI","family":"TAPIAINEN","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"TIIA","family":"KUJALA","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"TARJA","family":"KAIJALAINEN","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"IRMA","family":"IK\u00c4HEIMO","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"ANNIKA","family":"SAUKKORIIPI","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"MARJO","family":"RENKO","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"JARMO","family":"SALO","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"MAIJA","family":"LEINONEN","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"MATTI","family":"UHARI","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2010,3,20]]},"reference":[{"key":"e_1_2_6_2_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1462-5822.2009.01323.x"},{"key":"e_1_2_6_3_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI200320365"},{"key":"e_1_2_6_4_2","doi-asserted-by":"crossref","first-page":"2521","DOI":"10.1128\/AAC.42.10.2521","article-title":"Interaction of Streptococcus pneumoniae and Moraxella catarrhalis: investigation of the indirect pathogenic role of beta\u2010lactamase\u2010producing moraxellae by use of a continuous\u2010culture biofilm system","volume":"42","author":"Budhani RK","year":"1998","journal-title":"Antimicrob Agents Chemother"},{"key":"e_1_2_6_5_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/40.4.601"},{"key":"e_1_2_6_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.70.8.4980-4988.2004"},{"key":"e_1_2_6_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.00673-06"},{"key":"e_1_2_6_8_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2006.05310.x"},{"key":"e_1_2_6_9_2","doi-asserted-by":"publisher","DOI":"10.1001\/jama.287.13.1710"},{"key":"e_1_2_6_10_2","doi-asserted-by":"publisher","DOI":"10.1086\/381765"},{"key":"e_1_2_6_11_2","doi-asserted-by":"publisher","DOI":"10.1001\/jama.296.2.202"},{"key":"e_1_2_6_12_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.39.3.1021-1024.2001"},{"key":"e_1_2_6_13_2","doi-asserted-by":"publisher","DOI":"10.1097\/00006454-200107000-00004"},{"key":"e_1_2_6_14_2","doi-asserted-by":"crossref","first-page":"589","DOI":"10.1128\/jcm.11.6.589-592.1980","article-title":"Comparison of counterimmunoelectrophoresis and the capsular reaction test for typing of pneumococci","volume":"11","author":"Henrichsen J","year":"1980","journal-title":"J Clin Microbiol"},{"key":"e_1_2_6_15_2","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2180-8-173"},{"key":"e_1_2_6_16_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkl482"},{"key":"e_1_2_6_17_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.01369-06"},{"key":"e_1_2_6_18_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.45.1.166-169.2001"},{"key":"e_1_2_6_19_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.73.1.431-435.2005"},{"key":"e_1_2_6_20_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.juro.2006.08.081"},{"key":"e_1_2_6_21_2","doi-asserted-by":"publisher","DOI":"10.5414\/CNP71501"},{"key":"e_1_2_6_22_2","doi-asserted-by":"publisher","DOI":"10.1086\/593186"},{"key":"e_1_2_6_23_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkh479"},{"key":"e_1_2_6_24_2","doi-asserted-by":"publisher","DOI":"10.1097\/01.mlg.0000172036.82897.d4"},{"key":"e_1_2_6_25_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00405-009-0940-9"},{"key":"e_1_2_6_26_2","doi-asserted-by":"publisher","DOI":"10.3109\/17453670902947457"}],"container-title":["APMIS"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fj.1600-0463.2010.02587.x","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/j.1600-0463.2010.02587.x","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,31]],"date-time":"2023-10-31T10:21:57Z","timestamp":1698747717000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/j.1600-0463.2010.02587.x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2010,3,20]]},"references-count":25,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2010,4]]}},"alternative-id":["10.1111\/j.1600-0463.2010.02587.x"],"URL":"https:\/\/doi.org\/10.1111\/j.1600-0463.2010.02587.x","archive":["Portico"],"relation":{},"ISSN":["0903-4641","1600-0463"],"issn-type":[{"value":"0903-4641","type":"print"},{"value":"1600-0463","type":"electronic"}],"subject":[],"published":{"date-parts":[[2010,3,20]]},"assertion":[{"value":"2010-03-20","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}