{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,7]],"date-time":"2026-05-07T04:08:01Z","timestamp":1778126881888,"version":"3.51.4"},"reference-count":21,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2010,5,31]],"date-time":"2010-05-31T00:00:00Z","timestamp":1275264000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["APMIS"],"published-print":{"date-parts":[[2010,9]]},"abstract":"<jats:p>Savoia D, Donalisio M, Civra A, Salvadori S, Guerrini R. <jats:italic>In vitro<\/jats:italic> activity of dermaseptin S1 derivatives against genital pathogens. APMIS 2010; 118: 674\u201380.<\/jats:p><jats:p>The aim of this study was to evaluate the biological activity of nine dermaseptin\u2010S1 (DRS\u2010S1) derivatives (synthesized by solid\u2010phase methods and purified) against different pathogens causing genital infections (<jats:italic>Trichomonas vaginalis<\/jats:italic>, <jats:italic>Herpes simplex<\/jats:italic> virus, Papillomavirus). The <jats:italic>in vitro<\/jats:italic> activity on <jats:italic>T.\u00a0vaginalis<\/jats:italic> was determined by counting the protozoon in a hemocytometer after vital staining with trypan blue; antiviral activity of the compounds was tested on monolayers of Vero cells for <jats:italic>Herpes simplex virus\u20101<\/jats:italic> (GFP) and on 293TT cells for human papillomavirus (HPV\u201016) pseudovirions (GFP). The cytotoxicity of the derivatives was assessed by evaluating both the hemolytic activity and the effect on Vero and 293TT cells. The DRS\u2010S1 longer peptides demonstrated a superior activity on <jats:italic>T.\u00a0vaginalis<\/jats:italic> but also a certain cytopathic effect<jats:italic>.<\/jats:italic> The compounds with 29 amino acids exhibited activity against the two viruses tested at concentrations not toxic to cells. The results obtained show that some of the synthetic peptides assessed have inhibitory activity against the pathogens tested, indicating a potential for the development of new molecules for use as topical microbicides to prevent the sexual transmission of microorganisms.<\/jats:p>","DOI":"10.1111\/j.1600-0463.2010.02637.x","type":"journal-article","created":{"date-parts":[[2010,5,31]],"date-time":"2010-05-31T23:23:09Z","timestamp":1275348189000},"page":"674-680","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":20,"title":["<i>In vitro<\/i> activity of dermaseptin S1 derivatives against genital pathogens"],"prefix":"10.1111","volume":"118","author":[{"given":"DIANELLA","family":"SAVOIA","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"MANUELA","family":"DONALISIO","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"ANDREA","family":"CIVRA","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"SEVERO","family":"SALVADORI","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"REMO","family":"GUERRINI","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2010,8,18]]},"reference":[{"key":"e_1_2_6_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.peptides.2008.06.017"},{"key":"e_1_2_6_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijantimicag.2004.09.005"},{"key":"e_1_2_6_4_2","doi-asserted-by":"crossref","first-page":"31635","DOI":"10.1016\/S0021-9258(18)31742-3","article-title":"The vertebrate peptide antibiotics dermaseptins have overlapping structural features but target specific microorganisms","volume":"269","author":"Mor A","year":"1994","journal-title":"J Biol Chem"},{"key":"e_1_2_6_5_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M209289200"},{"key":"e_1_2_6_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.diagmicrobio.2006.11.006"},{"key":"e_1_2_6_7_2","doi-asserted-by":"publisher","DOI":"10.1155\/2009\/452567"},{"key":"e_1_2_6_8_2","doi-asserted-by":"publisher","DOI":"10.1002\/jmv.2134"},{"key":"e_1_2_6_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.virol.2005.02.002"},{"key":"e_1_2_6_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bmc.2008.07.032"},{"key":"e_1_2_6_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0924-8579(99)00094-1"},{"key":"e_1_2_6_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijantimicag.2003.07.022"},{"key":"e_1_2_6_13_2","doi-asserted-by":"publisher","DOI":"10.2307\/3274682"},{"key":"e_1_2_6_14_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.01467-07"},{"key":"e_1_2_6_15_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkn223"},{"key":"e_1_2_6_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/0166-0934(88)90134-6"},{"key":"e_1_2_6_17_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00452-08"},{"key":"e_1_2_6_18_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.antiviral.2010.03.012"},{"key":"e_1_2_6_19_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cbpa.2007.03.016"},{"key":"e_1_2_6_20_2","doi-asserted-by":"publisher","DOI":"10.2174\/1389450024605445"},{"key":"e_1_2_6_21_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2672.2008.03942.x"},{"key":"e_1_2_6_22_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(08)70254-8"}],"container-title":["APMIS"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fj.1600-0463.2010.02637.x","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/j.1600-0463.2010.02637.x","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,31]],"date-time":"2023-10-31T07:09:25Z","timestamp":1698736165000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/j.1600-0463.2010.02637.x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2010,8,18]]},"references-count":21,"journal-issue":{"issue":"9","published-print":{"date-parts":[[2010,9]]}},"alternative-id":["10.1111\/j.1600-0463.2010.02637.x"],"URL":"https:\/\/doi.org\/10.1111\/j.1600-0463.2010.02637.x","archive":["Portico"],"relation":{},"ISSN":["0903-4641","1600-0463"],"issn-type":[{"value":"0903-4641","type":"print"},{"value":"1600-0463","type":"electronic"}],"subject":[],"published":{"date-parts":[[2010,8,18]]},"assertion":[{"value":"2010-08-18","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}