{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T18:06:11Z","timestamp":1790791571322,"version":"4.1.0"},"reference-count":9,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2010,10,11]],"date-time":"2010-10-11T00:00:00Z","timestamp":1286755200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["APMIS"],"published-print":{"date-parts":[[2010,12]]},"abstract":"<jats:p>Bank S, Nielsen HM, Hoyer Mathiasen B, Christiansen Leth D, Hagelskj\u00e6r Kristensen L, Prag J. <jats:italic>Fusobacterium necrophorum<\/jats:italic>\u2013 detection and identification on a selective agar. APMIS 2010; 118: 994\u20139.<\/jats:p><jats:p>Within the last decade, <jats:italic>Fusobacterium necrophorum<\/jats:italic> subsp. <jats:italic>funduliforme<\/jats:italic> has been considered a clinically important pathogen causing pharyngitis especially in adolescents and young adults. <jats:italic>F. necrophorum<\/jats:italic> pharyngitis can progress into Lemierre\u2019s syndrome, which is a severe and life\u2010threatening infection. However, throat swabs are not cultured anaerobically in the routine and even if cultured anaerobically, it can be difficult to identify <jats:italic>F. necrophorum<\/jats:italic> from the normal flora of the throat. <jats:italic>F. necrophorum<\/jats:italic> is therefore often overlooked as the cause of pharyngitis. In our laboratory, a <jats:italic>F. necrophorum<\/jats:italic> selective agar has been developed containing vancomycin and nalidixin, which inhibit the growth of most Gram\u2010positive and many Gram\u2010negative bacteria, respectively. \u03b2\u2010haemolysis of horse blood can be detected, which further facilitates the detection and identification of <jats:italic>F. necrophorum<\/jats:italic>. The <jats:italic>F. necrophorum<\/jats:italic> selective agar was evaluated against a quantitative real\u2010time polymerase chain reaction assay and shown to have a significantly higher sensitivity for detecting <jats:italic>F. necrophorum<\/jats:italic> than the anaerobic agar commonly used in Denmark. Furthermore, the <jats:italic>F. necrophorum<\/jats:italic> selective agar does not require experienced laboratory technicians, require fewer subcultures, is probably less expensive and is faster to perform than other culture methods.<\/jats:p>","DOI":"10.1111\/j.1600-0463.2010.02683.x","type":"journal-article","created":{"date-parts":[[2010,10,12]],"date-time":"2010-10-12T03:56:21Z","timestamp":1286855781000},"page":"994-999","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":31,"title":["<i>Fusobacterium necrophorum<\/i>\u2013 detection and identification on a selective agar"],"prefix":"10.1111","volume":"118","author":[{"given":"STEFFEN","family":"BANK","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"HANNE MERETE","family":"NIELSEN","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"BORIS","family":"HOYER MATHIASEN","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"DORTE","family":"CHRISTIANSEN LETH","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"LENA","family":"HAGELSKJ\u00c6R KRISTENSEN","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J\u00d8RGEN","family":"PRAG","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2010,10,11]]},"reference":[{"key":"e_1_2_6_2_2","doi-asserted-by":"publisher","DOI":"10.1080\/09674845.2007.11732757"},{"key":"e_1_2_6_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jinf.2004.09.013"},{"key":"e_1_2_6_4_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-0691.2007.01719.x"},{"key":"e_1_2_6_5_2","doi-asserted-by":"publisher","DOI":"10.1086\/644616"},{"key":"e_1_2_6_6_2","doi-asserted-by":"publisher","DOI":"10.7326\/0003-4819-151-11-200912010-00011"},{"key":"e_1_2_6_7_2","doi-asserted-by":"publisher","DOI":"10.1007\/s10096-008-0468-8"},{"key":"e_1_2_6_8_2","doi-asserted-by":"publisher","DOI":"10.1099\/jmm.0.45648-0"},{"key":"e_1_2_6_9_2","doi-asserted-by":"publisher","DOI":"10.1080\/09674845.2005.11732687"},{"key":"e_1_2_6_10_2","doi-asserted-by":"publisher","DOI":"10.1136\/jcp.33.1.61"}],"container-title":["APMIS"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fj.1600-0463.2010.02683.x","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/j.1600-0463.2010.02683.x","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,31]],"date-time":"2023-10-31T18:34:34Z","timestamp":1698777274000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/j.1600-0463.2010.02683.x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2010,10,11]]},"references-count":9,"journal-issue":{"issue":"12","published-print":{"date-parts":[[2010,12]]}},"alternative-id":["10.1111\/j.1600-0463.2010.02683.x"],"URL":"https:\/\/doi.org\/10.1111\/j.1600-0463.2010.02683.x","archive":["Portico"],"relation":{},"ISSN":["0903-4641","1600-0463"],"issn-type":[{"value":"0903-4641","type":"print"},{"value":"1600-0463","type":"electronic"}],"subject":[],"published":{"date-parts":[[2010,10,11]]},"assertion":[{"value":"2010-10-11","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}