{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,26]],"date-time":"2026-08-26T20:33:30Z","timestamp":1787776410257,"version":"build-2784847793"},"reference-count":35,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2011,10,17]],"date-time":"2011-10-17T00:00:00Z","timestamp":1318809600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["APMIS"],"published-print":{"date-parts":[[2011,12]]},"abstract":"<jats:p>Titelman E, Iversen A, Kahlmeter G, Giske CG. Antimicrobial susceptibility to parenteral and oral agents in a largely polyclonal collection of CTX\u2010M\u201014 and CTX\u2010M\u201015\u2010producing <jats:italic>Escherichia coli<\/jats:italic> and <jats:italic>Klebsiella pneumoniae.<\/jats:italic> APMIS 2011; 119: 853\u201363.<\/jats:p><jats:p>Activity of oral and parenteral antimicrobials against consecutively isolated extended\u2010spectrum \u03b2\u2010lactamase (ESBL)\u2010producing <jats:italic>Escherichia coli<\/jats:italic> (n\u2003=\u2003149) and <jats:italic>Klebsiella pneumoniae<\/jats:italic> (n\u2003=\u200320) was determined, and susceptibility test methods were compared for parenteral \u03b2\u2010lactams. Polymerase chain reaction (PCR) targeting <jats:italic>bla<\/jats:italic><jats:sub>CTX\u2010M<\/jats:sub>, <jats:italic>bla<\/jats:italic><jats:sub>SHV<\/jats:sub> and <jats:italic>bla<\/jats:italic><jats:sub>TEM<\/jats:sub>, and DNA sequencing and epidemiological typing with pulsed\u2010field gel electrophoresis were performed. PCR targeting <jats:italic>pabB<\/jats:italic> was screened for <jats:italic>E. coli<\/jats:italic> O25b\u2010ST131. Minimum inhibitory concentrations (MICs) were determined using Etest and broth microdilution. Disc diffusion was performed according to European Committee on Antimicrobial Susceptibility Testing (EUCAST). Dominating genotypes were <jats:italic>bla<\/jats:italic><jats:sub>CTX\u2010M\u201015<\/jats:sub> (75%) and <jats:italic>bla<\/jats:italic><jats:sub>CTX\u2010M\u201014<\/jats:sub> (23%). Four <jats:italic>E. coli<\/jats:italic> clusters (7\u201318 isolates) were found. Forty\u2010two per cent of <jats:italic>E. coli<\/jats:italic> belonged to O25b\u2010ST131. Ciprofloxacin resistance was 72%, trimethoprim resistance was 70%. Among <jats:italic>E. coli<\/jats:italic>, resistance to mecillinam (13%), nitrofurantoin (7%) and fosfomycin (3%) was low, although resistance was high in <jats:italic>K. pneumoniae<\/jats:italic> (25%, 60%, 85%). Susceptibility to ertapenem was 99%, piperacillin\u2010tazobactam 91%, tigecycline 96% and temocillin 76%. Susceptibility rates obtained with broth microdilution and Etest were in agreement for cefotaxime (2 vs 1%) and ceftazidime (9 vs 11%), but not for piperacillin\u2010tazobactam (59 vs 91%). With disc diffusion major errors occurred with piperacillin\u2010tazobactam (18\/169). Several therapeutic alternatives exist for ESBL\u2010producing <jats:italic>E. coli<\/jats:italic>, but few exist for <jats:italic>K. pneumoniae<\/jats:italic>. Disc diffusion and Etest can accurately predict susceptibility to cefotaxime and ceftazidime, but not to piperacillin\u2010tazobactam with the present breakpoints.<\/jats:p>","DOI":"10.1111\/j.1600-0463.2011.02766.x","type":"journal-article","created":{"date-parts":[[2011,10,17]],"date-time":"2011-10-17T12:33:30Z","timestamp":1318854810000},"page":"853-863","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":41,"title":["Antimicrobial susceptibility to parenteral and oral agents in a largely polyclonal collection of CTX\u2010M\u201014 and CTX\u2010M\u201015\u2010producing <i>Escherichia coli<\/i> and <i>Klebsiella pneumoniae<\/i>"],"prefix":"10.1111","volume":"119","author":[{"given":"EMILIA","family":"TITELMAN","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"AINA","family":"IVERSEN","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"GUNNAR","family":"KAHLMETER","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"CHRISTIAN G.","family":"GISKE","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2011,10,17]]},"reference":[{"key":"e_1_2_6_2_2","doi-asserted-by":"publisher","DOI":"10.2165\/11533040-000000000-00000"},{"key":"e_1_2_6_3_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkl483"},{"key":"e_1_2_6_4_2","doi-asserted-by":"crossref","first-page":"225","DOI":"10.2807\/esm.12.03.00693-en","article-title":"Short summary of Swedres 2005, a report on Swedish antibiotic utilisation and resistance","volume":"12","author":"Cars O","year":"2007","journal-title":"Euro Surveill"},{"key":"e_1_2_6_5_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0463.2007.apm_547.x"},{"key":"e_1_2_6_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.01943-07"},{"key":"e_1_2_6_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.42.12.5917-5920.2004"},{"key":"e_1_2_6_8_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0463.2008.00922.x"},{"key":"e_1_2_6_9_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dki166"},{"key":"e_1_2_6_10_2","doi-asserted-by":"publisher","DOI":"10.1086\/503264"},{"key":"e_1_2_6_11_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-0691.2007.01864.x"},{"key":"e_1_2_6_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(08)70041-0"},{"key":"e_1_2_6_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jinf.2006.09.004"},{"key":"e_1_2_6_14_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkl043"},{"key":"e_1_2_6_15_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-0691.2007.01852.x"},{"key":"e_1_2_6_16_2","doi-asserted-by":"publisher","DOI":"10.1001\/archinte.168.17.1897"},{"key":"e_1_2_6_17_2","unstructured":"TasbakanHP \u2003SO YamazhanT UlusoyS.Nitrofurantoin in the treatment of extended\u2010spectrum \u03b2 \u2010lactamase producing E. coli related lower urinary tract infection. 48th Annual ICAAC\/IDSA 46th Annual Meeting October 25\u201328 2008 Washington 2008."},{"key":"e_1_2_6_18_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dki451"},{"key":"e_1_2_6_19_2","unstructured":"EUCAST.Breakpoint tables for interpretation of MICs and zone diameters.2011.http:\/\/www.eucast.org\/clinical_breakpoints(accessed 14 February 2011)."},{"key":"e_1_2_6_20_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1469-0691.2000.00142.x"},{"key":"e_1_2_6_21_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkm467"},{"key":"e_1_2_6_22_2","unstructured":"EUCAST.Disk diffusion test.2011.http:\/\/www.eucast.org\/eucast_disk_diffusion_test\/(accessed 16 February 2011)."},{"key":"e_1_2_6_23_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0463.2004.apm11211-1208.x"},{"key":"e_1_2_6_24_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkm316"},{"key":"e_1_2_6_25_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.74.12.5463"},{"key":"e_1_2_6_26_2","unstructured":"BLAST.Basic Local Alignment Search Tool.2008.http:\/\/www.ncbi.nlm.nih.gov\/blast(accessed 19 December 2008)."},{"key":"e_1_2_6_27_2","unstructured":"PulseNet [database on the Internet] 2008.http:\/\/www.cdc.gov\/pulsenet\/protocols.htm(accessed 17 December 2008)."},{"key":"e_1_2_6_28_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.33.9.2233-2239.1995"},{"key":"e_1_2_6_29_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkp194"},{"key":"e_1_2_6_30_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkm464"},{"key":"e_1_2_6_31_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.mimet.2010.09.004"},{"key":"e_1_2_6_32_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-0691.2007.01856.x"},{"key":"e_1_2_6_33_2","unstructured":"CLSI.Performance Standards for Antimicrobial Susceptibility Testing; Twentieth Informational Supplement. M100\u2010S20.2010.Clinical and Laboratory Standard Institute Wayne PA."},{"key":"e_1_2_6_34_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.39.6.2206-2212.2001"},{"key":"e_1_2_6_35_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.01319-06"},{"key":"e_1_2_6_36_2","unstructured":"EUCAST.Zone diameter distributions.http:\/\/www.eucast.org\/zone_diameter_distributions\/(accessed 14 February 2011)."}],"container-title":["APMIS"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fj.1600-0463.2011.02766.x","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/j.1600-0463.2011.02766.x","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,15]],"date-time":"2023-10-15T19:07:52Z","timestamp":1697396872000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/j.1600-0463.2011.02766.x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2011,10,17]]},"references-count":35,"journal-issue":{"issue":"12","published-print":{"date-parts":[[2011,12]]}},"alternative-id":["10.1111\/j.1600-0463.2011.02766.x"],"URL":"https:\/\/doi.org\/10.1111\/j.1600-0463.2011.02766.x","archive":["Portico"],"relation":{},"ISSN":["0903-4641","1600-0463"],"issn-type":[{"value":"0903-4641","type":"print"},{"value":"1600-0463","type":"electronic"}],"subject":[],"published":{"date-parts":[[2011,10,17]]},"assertion":[{"value":"2011-10-17","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}