{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,2]],"date-time":"2025-12-02T15:21:05Z","timestamp":1764688865177},"reference-count":23,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2012,3,24]],"date-time":"2012-03-24T00:00:00Z","timestamp":1332547200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["APMIS"],"published-print":{"date-parts":[[2012,9]]},"abstract":"<jats:p>A plasmid\u2010encoded <jats:styled-content style=\"fixed-case\"><jats:roman>ClpK<\/jats:roman><\/jats:styled-content> protein was recently identified as a predictor of a heat\u2010resistant phenotype in the opportunistic pathogen <jats:italic><jats:styled-content style=\"fixed-case\">K<\/jats:styled-content>lebsiella pneumoniae<\/jats:italic>. This study was undertaken to evaluate the presence of the <jats:styled-content style=\"fixed-case\"><jats:italic>clpK<\/jats:italic><\/jats:styled-content> gene in extended\u2010spectrum \u03b2\u2010lactamase (<jats:styled-content style=\"fixed-case\">ESBL<\/jats:styled-content>)\u2010producing <jats:italic><jats:styled-content style=\"fixed-case\">K<\/jats:styled-content>. pneumoniae<\/jats:italic> and to assess the probable co\u2010transfer of multi\u2010resistance with the heat resistance phenotype. A <jats:styled-content style=\"fixed-case\">D<\/jats:styled-content>anish collection of 80 <jats:styled-content style=\"fixed-case\">ESBL<\/jats:styled-content>\u2010producing <jats:italic><jats:styled-content style=\"fixed-case\">K<\/jats:styled-content>. pneumoniae<\/jats:italic> bloodstream infection isolates was screened for <jats:styled-content style=\"fixed-case\"><jats:italic>clpK<\/jats:italic><\/jats:styled-content> by colony hybridization. Nineteen isolates (24%) were positive for <jats:styled-content style=\"fixed-case\"><jats:italic>clpK<\/jats:italic><\/jats:styled-content>; some of them representing major clones identified in <jats:styled-content style=\"fixed-case\">D<\/jats:styled-content>enmark. Among these, nine isolates belonged to a single <jats:italic><jats:styled-content style=\"fixed-case\">K<\/jats:styled-content>. pneumoniae <\/jats:italic><jats:styled-content style=\"fixed-case\">CTX<\/jats:styled-content>\u2010M\u201015 clone with sequence type (<jats:styled-content style=\"fixed-case\">ST<\/jats:styled-content>)16 exhibiting a heat\u2010resistant phenotype. This clone has a multi\u2010hospital occurrence and has also been detected outside <jats:styled-content style=\"fixed-case\">D<\/jats:styled-content>enmark. Horizontal co\u2010transfer of multiple antibiotic resistances, including the <jats:styled-content style=\"fixed-case\">CTX<\/jats:styled-content>\u2010M\u201015 resistance determinant, and the heat resistance phenotype was observed. Thus, the <jats:styled-content style=\"fixed-case\"><jats:italic>clpK<\/jats:italic><\/jats:styled-content> gene is harbored by different <jats:styled-content style=\"fixed-case\">ESBL<\/jats:styled-content>\u2010producing <jats:italic><jats:styled-content style=\"fixed-case\">K<\/jats:styled-content>. pneumoniae<\/jats:italic> isolates including a clone of <jats:styled-content style=\"fixed-case\">ST<\/jats:styled-content>16 internationally spread. The co\u2010localization of <jats:styled-content style=\"fixed-case\"><jats:italic>clpK<\/jats:italic><\/jats:styled-content> on transferable <jats:styled-content style=\"fixed-case\">ESBL<\/jats:styled-content>\u2010encoding plasmids allowing co\u2010dissemination of multiple drug resistance with bacterial heat resistance is a highly interesting phenomenon that may further complicate the prevention of spreading of certain successful clones of multi\u2010resistant <jats:italic><jats:styled-content style=\"fixed-case\">K<\/jats:styled-content>. pneumoniae<\/jats:italic>.<\/jats:p>","DOI":"10.1111\/j.1600-0463.2012.02885.x","type":"journal-article","created":{"date-parts":[[2012,3,24]],"date-time":"2012-03-24T14:30:36Z","timestamp":1332599436000},"page":"699-705","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":20,"title":["Concurrent emergence of multidrug resistance and heat resistance by <scp>CTX\u2010M\u201015<\/scp>\u2010encoding conjugative plasmids in <i><scp>K<\/scp>lebsiella pneumoniae<\/i>"],"prefix":"10.1111","volume":"120","author":[{"given":"Martin S.","family":"Bojer","sequence":"first","affiliation":[{"name":"Department of Microbiological Surveillance and Research Statens Serum Institut Copenhagen S Denmark"},{"name":"Department of Science Systems and Models Roskilde University Roskilde Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anette M.","family":"Hammerum","sequence":"additional","affiliation":[{"name":"Department of Microbiological Surveillance and Research Statens Serum Institut Copenhagen S Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Steffen L.","family":"J\u00f8rgensen","sequence":"additional","affiliation":[{"name":"Department of Microbiological Surveillance and Research Statens Serum Institut Copenhagen S Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Frank","family":"Hansen","sequence":"additional","affiliation":[{"name":"Department of Microbiological Surveillance and Research Statens Serum Institut Copenhagen S Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stefan S.","family":"Olsen","sequence":"additional","affiliation":[{"name":"Department of Microbiological Surveillance and Research Statens Serum Institut Copenhagen S Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Karen A.","family":"Krogfelt","sequence":"additional","affiliation":[{"name":"Department of Microbiological Surveillance and Research Statens Serum Institut Copenhagen S Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Carsten","family":"Struve","sequence":"additional","affiliation":[{"name":"Department of Microbiological Surveillance and Research Statens Serum Institut Copenhagen S Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2012,3,24]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.11.4.589"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.3201\/eid1307.070187"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkl483"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijantimicag.2009.11.003"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkm403"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkp211"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijantimicag.2011.03.020"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkn287"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijantimicag.2011.03.018"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0015467"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1099\/jmm.0.032698-0"},{"key":"e_1_2_7_13_1","unstructured":"The European Committee on Antimicrobial Susceptibility Testing.Breakpoint tables for interpretation of MICs and zone diameters. 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