{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,27]],"date-time":"2025-09-27T22:17:25Z","timestamp":1759011445670},"reference-count":22,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2012,6,28]],"date-time":"2012-06-28T00:00:00Z","timestamp":1340841600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["APMIS"],"published-print":{"date-parts":[[2013,1]]},"abstract":"<jats:p>The factor <jats:styled-content style=\"fixed-case\">H<\/jats:styled-content> binding protein (<jats:styled-content style=\"fixed-case\">fHbp<\/jats:styled-content>) is currently being evaluated in clinical trials as a vaccine candidate for a meningococcal group <jats:styled-content style=\"fixed-case\">B<\/jats:styled-content> vaccine. We have previously described the prevalence and sequence variation of <jats:italic>\n<jats:styled-content style=\"fixed-case\">fHbp<\/jats:styled-content>\n<\/jats:italic> (Jacobsson et\u00a0al., 2009) and here we investigate the expression of the antigen. The present study includes isolates from carriers (n\u00a0=\u00a062) and patients with invasive <jats:italic>Neisseria meningitidis<\/jats:italic> infections (n\u00a0=\u00a0146), of which 62 had a fatal outcome. Among the invasive isolates from patients with fatal and non\u2010fatal infections <jats:italic>\n<jats:styled-content style=\"fixed-case\">fHbp<\/jats:styled-content>\n<\/jats:italic> allele 1 was most common (42% and 29% respectively), but it was only identified in 3% of the carrier isolates, where allele 16 was most frequent (13%). The Fluorescence\u2010activated cell sorting analysis identified <jats:styled-content style=\"fixed-case\">fHbp<\/jats:styled-content> expression in all except seven isolates and further analysis by Western blot showed that five of these seven samples were indeed negative using a polyclonal anti\u2010<jats:styled-content style=\"fixed-case\">fHbp<\/jats:styled-content> serum. The negative isolates belonged to serogroup <jats:styled-content style=\"fixed-case\">B <\/jats:styled-content>\n<jats:italic>\n<jats:styled-content style=\"fixed-case\">fHbp<\/jats:styled-content>\n<\/jats:italic> allele 24, Y allele 104, and W\u2010135 allele 16 (all invasive). Two were non\u2010serogroupable carrier isolates (allele 21 and 101). An interesting finding is that isolates from invasive infections with fatal outcome had lower expression of <jats:styled-content style=\"fixed-case\">fHbp<\/jats:styled-content> or lower affinity for the <jats:styled-content style=\"fixed-case\">fHbp<\/jats:styled-content> antibody compared to isolates from non\u2010fatal invasive infections and carriers.<\/jats:p>","DOI":"10.1111\/j.1600-0463.2012.02934.x","type":"journal-article","created":{"date-parts":[[2012,6,28]],"date-time":"2012-06-28T12:29:17Z","timestamp":1340886557000},"page":"56-63","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Gene variability and degree of expression of vaccine candidate factor <scp>H<\/scp> binding protein in clinical isolates of <i><scp>N<\/scp>eisseria meningitidis<\/i>"],"prefix":"10.1111","volume":"121","author":[{"given":"Anne","family":"Kelly","sequence":"first","affiliation":[{"name":"Department of Clinical Medicine School 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